Biochemical Profiles of In Vivo Oral Mucosa by Using a Portable Raman Spectroscopy System

Nogueira, Marcelo Saito; Ribeiro, Victoria Stadler Tasca; Pires, Marianna; Peralta, Felipe da Silva; Carvalho, Luis Felipe das Chagas e Silva de

doi:10.3390/opt2030013

Cited by 10 publications

(2 citation statements)

References 63 publications

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“…Both computer-based exercises and lab practical activities covered the same topics (tissue absorption, scattering, effective attenuation, reflectance spectra, tissue oxygenation) in order to make it easier for students to achieve the learning outcomes related to measurements and simulations. The complete guidelines to install and use our Tissue Optics app can be found at [57,58].…”

Section: Computer-based Activitiesmentioning

confidence: 99%

Teaching and learning in biophotonics: Crossing the bridge between educators and students

Nogueira,

Gunther,

Komolibus

et al. 2024

Journal of Biophotonics

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As a rapidly growing field, biophotonics demonstrates an increasingly higher demand for interdisciplinary professionals and requires the implementation of a structured approach to educational and outreach activities focused on appropriate curriculum, and teaching and learning for audiences with diverse technical backgrounds and learning styles. Our study shows the main findings upon applying this approach to biophotonics workshops delivered 2 consecutive years while updating and improving learning outcomes, teaching strategies, workshop content based on student and teacher feedback. We provided resources for a variety of lecture‐based, experimental, computer simulation activities. Quality of subject matter, teaching, and overall learning was rated as “Very good” or “Good” by 88%, 76%, and 82% of students in average, respectively. Application of our teaching strategies and materials during short‐ and long‐term workshops/courses could potentially increase the interest in pursuing careers in the biophotonics field and related areas, leading to standardized approaches in designing education and outreach events across centers.

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Section: Computer-based Activitiesmentioning

confidence: 99%

Teaching and learning in biophotonics: Crossing the bridge between educators and students

Nogueira,

Gunther,

Komolibus

et al. 2024

Journal of Biophotonics

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“…Therefore, scattering is associated with the tissue microstructure. On the other hand, absorption is associated with the tissue biomolecular composition, as its absorption bands are dependent on the molecular energy levels (including electronic [ 10 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 ], vibrational [ 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 ] and rotational levels [ 57 ]). Tissue absorption is related to biological chromophores (or absorbing biomolecules), which were previously investigated in a number of clinical and preclinical studies [ 58 ].…”

Section: Introductionmentioning

confidence: 99%

Insights into Biochemical Sources and Diffuse Reflectance Spectral Features for Colorectal Cancer Detection and Localization

Nogueira

Maryam

Amissah

et al. 2022

Cancers

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Colorectal cancer (CRC) is the third most common and second most deadly type of cancer worldwide. Early detection not only reduces mortality but also improves patient prognosis by allowing the use of minimally invasive techniques to remove cancer while avoiding major surgery. Expanding the use of microsurgical techniques requires accurate diagnosis and delineation of the tumor margins in order to allow complete excision of cancer. We have used diffuse reflectance spectroscopy (DRS) to identify the main optical CRC biomarkers and to optimize parameters for the integration of such technologies into medical devices. A total number of 2889 diffuse reflectance spectra were collected in ex vivo specimens from 47 patients. Short source-detector distance (SDD) and long-SDD fiber-optic probes were employed to measure tissue layers from 0.5 to 1 mm and from 0.5 to 1.9 mm deep, respectively. The most important biomolecules contributing to differentiating DRS between tissue types were oxy- and deoxy-hemoglobin (Hb and HbO2), followed by water and lipid. Accurate tissue classification and potential DRS device miniaturization using Hb, HbO2, lipid and water data were achieved particularly well within the wavelength ranges 350–590 nm and 600–1230 nm for the short-SDD probe, and 380–400 nm, 420–610 nm, and 650–950 nm for the long-SDD probe.

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Fundamentals and Applications of Raman‐Based Techniques for the Design and Development of Active Biomedical Materials

2023

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Raman spectroscopy is an analytical method based on light‐matter interactions that can interrogate the vibrational modes of matter and provide representative molecular fingerprints. Mediated by its label‐free, non‐invasive nature and high molecular specificity, Raman‐based techniques have become ubiquitous tools for in situ characterisation of materials. This review comprehensively describes the theoretical and practical background of Raman spectroscopy and its advanced variants. We highlight the numerous facets of material characterisation that Raman scattering can reveal, including biomolecular identification, solid‐to‐solid phase transitions and spatial mapping of biomolecular species in bioactive materials. The review illustrates the potential of these techniques in the context of active biomedical material design and development by highlighting representative studies from the literature. These studies cover the use of Raman spectroscopy for the characterisation of both natural and synthetic biomaterials, including engineered tissue constructs, biopolymer systems, ceramics and nanoparticle formulations, among others. To increase the accessibility and adoption of these techniques, the present review also provides the reader with practical recommendations on the integration of Raman techniques into the experimental laboratory toolbox. Finally, we provide perspectives on how recent developments in plasmon‐ and coherently‐enhanced Raman spectroscopy can propel Raman from underutilised to critical for biomaterial development.This article is protected by copyright. All rights reserved

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Biochemical Profiles of In Vivo Oral Mucosa by Using a Portable Raman Spectroscopy System

Cited by 10 publications

References 63 publications

Teaching and learning in biophotonics: Crossing the bridge between educators and students

Teaching and learning in biophotonics: Crossing the bridge between educators and students

Insights into Biochemical Sources and Diffuse Reflectance Spectral Features for Colorectal Cancer Detection and Localization

Fundamentals and Applications of Raman‐Based Techniques for the Design and Development of Active Biomedical Materials

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