Biochemical Profile of Paenibacillus larvae Repetitive Element Polymerase Chain Reaction (rep-PCR) Genotypes in Bulgaria

Rusenova, Nikolina; Parvanov, P.

doi:10.9775/kvfd.2013.9853

Cited by 3 publications

(2 citation statements)

References 14 publications

(30 reference statements)

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“…(M1HC1b) and Paenibacillus lautus (M1HC19 and M1HC27) at a similar metabolic pattern as reported by Kebria et al (2009) and Sharma et al ( 2015) (Table 3). Other species of Paenibacillus as P. larvae using the API 50CH and BioLog Gen III systems were positive for glycerol, D-ribose, D-glucose, D-fructose, D-mannose, D-mannitol, N-acetyl-D-glucosamine, arbutin, esculin, salicin, D-maltose, D-trehalose and gelatin (Kilwinski et al 2004, Rusenova andParvanov 2014).…”

Section: Bacterial Isolation and Characterizationmentioning

confidence: 99%

Archives of Environmental Protection

Mauricio-Gutiérrez¹,

Machorro-Velázquez²,

Jiménez-Salgado³

et al. 2020

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In Mexico, one of the principal natural resources is oil, however, the activity related to it has generated hydrocarbon spills on agricultural soils. The aim of this study was to evaluate the biodegradability of diesel by means of indigenous bacteria isolated from agricultural soil contaminated with 68 900 mg kg -1 diesel. We examined indigenous bacterial strains in agricultural soils contaminated with diesel from Acatzingo, Puebla, Mexico. We performed a physicochemical soil characterization, and a bacterial population quantification favoring sporulated bacteria of the genera Bacillus and Paenibacillus taken from the study site. Six bacterial strains were isolated. The identification was made based on the 16S rRNA gene and API systems. The tolerance and biodegradation capacity in diesel were determined at 4 000 to 24 000 mg L -1 of diesel. Residual concentrations of diesel were determined by GC-FID. Soil contaminated with diesel alters the concentrations of organic matter, phosphorus and nitrogen. Analysis of soil samples showed heat resistant bacterial populations of 10 6 cfu g -1 dry soil. Six strains from soil pollution were identified -Pseudomonas stutzeri M1CH1, Bacillus pumilus M1CH1b, Bacillus cereus M1CH10, Bacillus subtilis M1CH15a, and Paenibacillus lautus strains M1CH19 and M1CH27. These bacteria showed different degradation behavior. Bacillus pumilus M1HC1b and Paenibacillus lautus M1CH27 use diesel oil as the sole carbon source. Bacillus pumilus degraded high concentrations of diesel (24 000 mg L -1 ), while for Paenibacillus lautus it became toxic and the degradation was less.

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Section: Bacterial Isolation and Characterizationmentioning

confidence: 99%

Archives of Environmental Protection

Mauricio-Gutiérrez¹,

Machorro-Velázquez²,

Jiménez-Salgado³

et al. 2020

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“…PCR amplified products were examined on agarose (0.8%) gel electrophoresis. DNA amplified by PCR was stained with ethidium bromide and visualised with UV transilluminator [10][11][12]14,17,18] .…”

Section: Polymerase Chain Reaction (Pcr) Assaymentioning

confidence: 99%

Güney Marmara Bölgesindeki Bal Arılarının Yavru Çürüklüğü Hastalığı etkenlerinin PZR ve Kültürel Metodlar ile Belirlenmesi

Borum¹,

Özakın²,

Güneş³

et al. 2015

Kafkas Univ Vet Fak Derg

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American Foulbrood and European Foulbrood diseases of honeybees were examined in 725 beehives from 23 apiaries located in the South Marmara Region of Turkey. We determined that 19 apiaries were infected and the suspected clinical signs of foulbrood diseases were investigated in 102 beehives by PCR and cultural method. Broods and combs from colonies with suspected clinical symptoms of foulbrood diseases were collected and cultured for bacteriological examination. All of the specimens contaminated with bacteriae and 37 species of bacteriae were isolated such as Stapylococcus epidermidis, Bacillus subtilis, Corynebacterium jeikum, Corynebacterium pseudotuberculosis, Bacillus spp. All of these bacteria are related to human, animal and environmental origins. In this study, Paenibacillus larvae by PCR amplifying the 973-bp region PL1 and PL2 with 1f, Melissococcus plutonius amplifying the 973-bp region EFB-F and EFB-R gene were amplified. American Foulbrood causative agent Paenibacillus larvae and European Foulbrood causative agent Melissococcus plutonius were not detected in any sample examined by PCR and cultural methods. On the other hand, Paenibacillus alvei that is a seconder agent to European Foulbrood was found in two samples by cultural methods. In conclusion, the results showed that P. larvae and M. plutonius are not present in South Marmara Region. In this study, human, animal and environment originated agents were isolated. Keywords: Apis mellifera, Honeybee, Foulbrood, PCR Güney Marmara Bölgesindeki Bal Arılarının Yavru Çürüklüğü Hastalığı etkenlerinin PZR ve Kültürel Metodlar ile Belirlenmesi

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