Biochemical profile of callus cultures of Pogostemon cablin (Blanco) Benth

Abstract

Patchouli is an aromatic shrub of commercial interest because its essential oil is rich in patchoulol. This study aimed to evaluate the effect of growth regulators on callus production, analyze the essential oil production in calli and evaluate metabolic differences between callus, in vitro grown-plantlets and greenhouse-grown plants in three different accessions of patchouli. Calli were induced from leaf explants on media supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) in combination with 6-benzyladenine (BA). The largest size calli from different accessions were obtained in the presence of the two plant growth regulators (PGRs). For accession POG014, presence of 0.022 mg l -1 2,4-D plus 0.022 mg l -1 BA were optimum. For accession POG021, presence of 0.110 mg l -1 2,4-D plus 0.022 mg l -1 of BA induced the largest callus, whereas for accession POG002, 0.022 mg l -1 2,4-D and 0.225 mg l -1 BA, as well as 0.11 mg l -1 2,4-D and 0.022 mg l -1 BA promoted the development of largest callus. Among all accessions, peroxidase activity was highest in organogenic calli of accession POG014, whereas, polyphenol oxidase activity was highest in in vitro-grown plantlets of accession POG021. Biochemical variables differed significantly among the treatments, with the exception of total sugar levels. The highest concentrations of total sugars were observed in the calli and in vitro-grown plantlets of POG014 and POG021. Essential oils were not detected in callus tissues.

Compact callus cultures and evaluation of the antioxidant activity of Hovenia dulcis Thunb. (Rhamnaceae) under in vivo and in vitro culture conditions

“…In many species, the association of cytokinins and auxins has been reported to promote the best growth and maintenance of callus (Park et al, 2002;Salman, 2002;Santos et al, 2011).…”

Cytokinin Dehydrogenase activity in primary roots and characterization of primary metabolites from leaves and rootlets of Ricinus Communis

“…Thus, the observed differences in the levels of total sugars, reducing sugars and total amino acids between the leaves and radicles of castor seedlings could be attributed to the dual role of early seedlings first bodies, which provided storage and enable the photo-assimilation of macromolecules for the growth and maintenance of seedlings at 11-12 days after the germination under a natural photoperiod of 12 h of light and 12 h of darkness. Santos et al (2011) stated that the differences reported for different types of crops could be a result of the different metabolic requirements of these plants. Cordeiro (2007) stated that the processes that regulated the metabolism of the leaves determined the amount of molecules allocated to storage, maintenance and distribution.…”

Production of monoterpenoids and aroma compounds from cell suspension cultures of Camellia sinensis

“…Callus growth was the highest in MS medium supplemented with 4.42 mg l -1 2,4-D and 0.5 mg l -1 BA. Recently, the same combination of growth regulators, though at different concentrations, has been reported to be useful in initiation of callus tissues of Patchouli, an aromatic shrub of commercial interest (Santos et al 2011). …”