Biochemical polymorphisms in muscle and liver extracts and in the serum of the rainbow trout Salmo gairdneri

Abstract

The frequency of polymorphic phenotypes determined by starch gel electrophoresis from six enzyme systems was investigated on 664 rainbow trout (stock I) originating equally from six full-sib families. The enzyme systems studied were CA, AKP, G-6-PD, SOD, AEP, and 6-PGD.On material deriving from six parental matings totalling 212 offspring (stock 11) the mode of inheritance of the first four enzymes (AEP and 6-PGD were not polymorphic) and the additional systems IDH, PGM, Alb, and Psta, not analysed in stock I, were investigated.The G-6-PD system showed no polymorphism in the family material. The CA, PGM, Al.b, and Psta systems were easily identifiable. Their mode of inheritance with two alleles each can be considered as proven. For SOD three alleles, in four out of six possible progeny types, were found, for which the postulated mode of inheritance was confirmed.For IDH the mode of inheritance found by Allendorff & Utter (1973) was confirmed. This pattern shows two disomic gene loci, one of which is monomorphic, while the other carries four different alleles.The number of alleles and their mode of inheritance for the AEP system, which was not clearly identifiable, could not be elucidated.

Electrophoretic Variation of Proteins and Genetic Differentiation in Some Stocks of Rainbow Trout From Estonian Fish Hatcheries

“…is polymorphic (Diebig et al, 1979). Monomorphism has been reported in such systems as PGI-phosphoglucoseisomerase (Avise, Kjtto, 1973) and ADH-alcohol dehydrogenase (Allendorf et al, 1975;Busack et al, 1979;Guyomard, 1981).…”

“…E. Diebig et al (1979) have described the simple two-allele polymorphism of carbonic anhydrase (CA) in the rainbow trout liver, where p-naphthylacetate serves as the substrate of CA (usually it is also a substrate of unspecific esterases). We tried to detect CA polymorphisnrin .…”

“…Three alleles have been detected for this enzyme (Utter, Hodgins, 1972;Diebig et al, 1979;Guyomard, 1981). The third allele, however, is very rare in American rainbow trout populations.…”