Biochemical model for enhanced biological phosphorus removal

Comeau, Yves; Hall, Kenneth J.; Hancock, Robert E. W.; Oldham, W. K.

doi:10.1016/0043-1354(86)90115-6

Cited by 466 publications

(226 citation statements)

References 41 publications

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“…However, recognizing the apparent propensity for wild microbial consortia to synthesize the polymer (14)(15)(16), commercial production of PHA would theoretically appear to be a natural extension of wastewater treatment. In fact PHA synthesis is empirically associated with certain municipal wastewater treatment processes (17)(18)(19), although biological synthesis of PHA in full-scale wastewater treatment facilities, estimated at upwards of 4% (w/w) (data not shown), falls short of quantities necessary for commercial exploitation. Nevertheless, recognizing that many waste streams are rich in PHA precursors, the potential exists for PHA-production concurrent with wastewater treatment.…”

Section: Introductionmentioning

confidence: 99%

Functional Stability of a Mixed Microbial Consortium Producing PHA From Waste Carbon Sources

Thompson¹,

Coats²,

Smith³

et al. 2007

Applied Biochemistry and Biotecnology

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Polyhydroxyalkanoates (PHAs) represent an environmentally-effective alternative to synthetic thermoplastics; however, current production practices are not sustainable. In this study, PHA production was accomplished in sequencing batch bioreactors utilizing real wastewaters and mixed microbial consortia from municipal activated sludge as inoculum. Polymer production reached 85%, 53%, and 10% of the cell dry weight from methanol-enriched pulp-and-paper mill foul condensate, fermented municipal primary solids, and biodiesel wastewater, respectively. Employing denaturing gradient gel electrophoresis of 16S-rDNA from PCR-amplified DNA extracts, distinctly different communities were observed between and within wastewaters following enrichment.Most importantly, functional stability was maintained despite differing and contrasting microbial populations.

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Section: Introductionmentioning

confidence: 99%

Functional Stability of a Mixed Microbial Consortium Producing PHA From Waste Carbon Sources

Thompson¹,

Coats²,

Smith³

et al. 2007

Applied Biochemistry and Biotecnology

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“…from waste water (Fuhs & Chen, 1975 ;Marais et al, 1983). Empirical metabolic models (Comeau et al, 1986 ;Mino et al, 1987) suggested that EBPR is generally linked to the synthesis and consumption of intracellular storage polymers of phosphate (as polyphosphate) and carbon [as polyhydroxyalkanoates (PHA) or cellular carbohydrates]. In the anaerobic stage, the responsible micro-organisms may utilize energy derived from polyphosphate hydrolysis for carbon uptake and storage, and later under the aerobic stage, the previously stored carbon can be used for growth and for polyphosphate formation (Comeau et al, 1986 ;Mino et al, 1987).…”

Section: Introductionmentioning

confidence: 99%

“…Empirical metabolic models (Comeau et al, 1986 ;Mino et al, 1987) suggested that EBPR is generally linked to the synthesis and consumption of intracellular storage polymers of phosphate (as polyphosphate) and carbon [as polyhydroxyalkanoates (PHA) or cellular carbohydrates]. In the anaerobic stage, the responsible micro-organisms may utilize energy derived from polyphosphate hydrolysis for carbon uptake and storage, and later under the aerobic stage, the previously stored carbon can be used for growth and for polyphosphate formation (Comeau et al, 1986 ;Mino et al, 1987). The engineering mass-balance control strategy has been used to select microbial communities that carry out EBPR metabolism, but, unlike biological nitrification and denitrification processes, suitable operational conditions for EBPR process control have not been developed (Jenkins & Tandoi, 1991 ;Mino et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Microbial community changes in biological phosphate-removal systems on altering sludge phosphorus content The GenBank/EMBL/DDBJ accession numbers for the sequences obtained in this report are AF109792 (strain Lpha5), AF109793 (strain Lpha7) and AF124650 to AF124659.

et al. 2000

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Biomarkers (respiratory quinones and cellular fatty acids) and denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S rRNA genes were used to characterize the microbial community structure of lab-scale enhanced biological phosphate-removal (EBPR) systems in response to altering sludge phosphorus (P) content. All the data suggest that the microbial community structures of sludge samples with a P content between 8 and 123 % (sludge dry weight) (i.e. good EBPR activity) were very similar, but differed from those with 2 % P content (i.e. no EBPR activity). For all samples analysed, ubiquinones Q-8 and Q-10, menaquinone MK-8(H 4 ), and fatty acids C 16 :0 , C 16 :1 ω9c and C 18 :1 ω11c were the major components. The dominance of Q-8, Q-10 and MK-8 (H 4 ) suggested that large numbers of organisms belonging to the β and α subclasses of the Proteobacteria and the Actinobacteria from the high GMC Gram-positive bacteria, respectively, were present. DGGE analysis revealed at least 7-9 predominant DNA bands and numerous other fragments in each sample. Five major DGGE fragments from each of the 2 % and 12 % Pcontaining sludge samples, respectively, were successfully isolated and sequenced. Phylogenetic analysis of the sequences indicated that both 2 % and 12 % P-containing sludge samples shared three common phylotypes that were separately affiliated with a novel bacterial group from the γ subclass of the Proteobacteria, two MK-8(H 4 )-containing actinobacteria previously isolated from the 2 % P-containing sludge, and a Caulobacter spp. in the α subclass of the Proteobacteria. The phylogenetic analysis also revealed phylotypes unique to both sludge samples. Changes in sludge P content therefore had an effect on the composition and abundance of the predominant microbial populations, though specific phylotypes could not be unequivocally associated with EBPR.

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“…While the presence of PHB was unequivocally demonstrated in sludge actively removing phosphate (Comeau et al 1986), most routine monitoring of activated sludge systems for PHB metabolism involves microscopic examination of sludges stained with Sudan black (LOtter and Murphy 1988). Care must therefore be taken when interpreting microscopic data from such systems.…”

Section: Effects Of Nutrient Limitation On Phb Productionmentioning

confidence: 99%

“…(Buchan 1983). A model for the overall process involves breakdown of polyphosphate and production of poly-fl-hydroxybutyrate (PHB) during the anaerobic stage and breakdown of P H B and synthesis of polyphosphate in the subsequent aerobic stage (Comeau et al 1986). Lawson and Tonhazy (1980) have inferred the presence of P H B in Acinetobacter sp.…”

Section: Introductionmentioning

confidence: 99%

Production of poly-\-hydroxybutyrate in Acinetobacter spp. isolated from activated sludge

Rees

Vasiliadis

May

et al. 1993

Appl Microbiol Biotechnol

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Two strains of Acinetobacter sp. isolated from activated sludge actively removing phosphate were examined for their abilities to produce poly-fl-hydroxybutyrate (PHB). When yield-limited by phosphate, strain RA3117 contained material that stained with Sudan Black, but contained only 0.9% PHB on a dry weight basis. This strain contained no sudanophilic material or PHB when limited by ammonia or sulphate. When strain RA3757 was limited by phosphate, ammonia or sulphate it produced 2.0, 7.8 and 11.5°70 PHB, respectively, on a dry weight basis, fl-Ketothiolase and acetoacetyl-coenzyme A (CoA) reductase were only observed in RA3757 cell-free extracts, fl-Ketothiolase was produced both in cells with and without P H B whereas acetoacetylrCoA reductase was found only in cells accumulating PHB. When RA3757 was grown in ammonialimiting medium with acetate, butyrate, caproate or ethanol as carbon source, similar levels of PHB were produced. When cells were grown on valerate, RA3757 produced 5.6 poly-fl-hydroxyvalerate and 0.9% PHB on a dry weight basis.

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Biochemical model for enhanced biological phosphorus removal

Cited by 466 publications

References 41 publications

Functional Stability of a Mixed Microbial Consortium Producing PHA From Waste Carbon Sources

Functional Stability of a Mixed Microbial Consortium Producing PHA From Waste Carbon Sources

Microbial community changes in biological phosphate-removal systems on altering sludge phosphorus content The GenBank/EMBL/DDBJ accession numbers for the sequences obtained in this report are AF109792 (strain Lpha5), AF109793 (strain Lpha7) and AF124650 to AF124659.

Production of poly-\-hydroxybutyrate in Acinetobacter spp. isolated from activated sludge

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