Biochemical Methods to Analyze the Subcellular Localization of NF-κB Proteins Using Cell Fractionation

Vecchiotti, Davide; Verzella, Daniela; Capece, Daria; Nolfi, Mauro Di Vito; Francesco, Barbara Di; Cornice, Jessica; Franzoso, Guido; Alesse, Edoardo; Zazzeroni, Francesca

doi:10.1007/978-1-0716-1669-7_2

Cited by 1 publication

(1 citation statement)

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“…MIC-1 decreased the NF-κB's nuclear translocation, as visualized by immunofluorescence staining. This observation may be validated further with cell fractionation experiments [35]. The observed MIC-1's anti-inflammatory effects in skeletal muscle cells were similar to its effects in macrophages, which suggest that the oral administration of MIC-1 affects multiple inflammation-mediated processes [8].…”

Section: Plos Onementioning

confidence: 60%

Moringa isothiocyanate-1 inhibits LPS-induced inflammation in mouse myoblasts and skeletal muscle

et al. 2022

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This study aims to investigate the anti-inflammatory effects of moringa isothiocyanate-1 (MIC-1) extracted from seeds of Moringa oleifera Lam. in lipopolysaccharide (LPS)-induced inflammation models. MIC-1 decreased nitric oxide production and reduced the expression of pro-inflammatory markers (TNF-α, Ifn-α, IL-1β, IL-6) in C2C12 myoblasts. The daily oral treatment of MIC-1 (80 mg/kg) for three days significantly reduced the expression of pro-inflammatory markers in gastrocnemius muscle tissue of LPS-treated C57BL/6 male mice. Transcriptomic analysis provided further insights into the inhibitory effects of MIC-1 on the LPS-induced inflammation, which suggested that MIC-1 affects inflammation and immunity-related genes in myoblasts and skeletal muscle tissue. MIC-1 inhibited the nuclear accumulation of the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) in the LPS-treated myoblasts. Our data support the hypothesis that the MIC-1’s effects in the muscle cells are mediated through the inhibition of the NF-κB translocation in the nucleus, which, in turn, results in immunomodulating and anti-inflammatory responses at the gene expression levels.

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Section: Plos Onementioning

confidence: 60%