Biochemical mechanisms of dose-dependent cytotoxicity and ROS-mediated apoptosis induced by lead sulfide/graphene oxide quantum dots for potential bioimaging applications

Ayoubi, Mahdi; Naserzadeh, Parvaneh; Hashemi, Mohammad Taghi; Rostami, Mohammad Reza; Tamjid, Elnaz; Tavakoli, Mohammad Mahdi; Simchi, A.

doi:10.1038/s41598-017-13396-y

Cited by 52 publications

(40 citation statements)

References 49 publications

(46 reference statements)

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“…Quantum dots, due to their extraordinary properties, have a great application in medicine and pharmacy, especially in cell labelling and long-term bioimaging. However, these nanomaterials have a tendency for reactive oxygen species generation [2,11,27,28,43] which negatively affects cell viability and biochemical processes, thus, significantly interrupting real-time investigations [2,27,28,43,44]. ROS cause lipid peroxidation, proteis, nucleic acid, or carbohydrate damage, and oxidative stress, which leads to cell apoptosis or necrosis as a consequence of metabolism product oxidation.…”

Section: Antioxidant Activity Studymentioning

confidence: 99%

“…A promising tool are quantum dots (QDs) which can be described as objects of the size below 10 nm with unique properties [1][2][3][4]. Since their development, they have been successfully used in optoelectronics [1], food packaging [5], metals detection [6][7][8], bioimaging [9][10][11][12][13][14], photocatalysis [15,16], sensing [17][18][19][20][21][22][23], cell labelling [24], or fluorescent inks and others [25,26]. The first-generation quantum dots are semiconductors of crystalline structure [3].…”

Section: Introductionmentioning

confidence: 99%

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Smart, Tunable CQDs with Antioxidant Properties for Biomedical Applications—Ecofriendly Synthesis and Characterization

et al. 2020

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Carbon quantum dots (CQDs) are nanoobjects of a size below 10 nm. Due to their favorable features, such as tunable luminescence, unique optical properties, water solubility, and lack of cytotoxicity, they are willingly applied in biomedicine. They can be obtained via bottom-up and top-down methods. However, to increase their quantum yield they must undergo post-processing. The aim of the following research was to obtain a new type of CQDs modified with a rhodamine b derivative to enhance their fluorescence performance without biocompability deterioration. For their preparation glucose was used as a precursor and four different carbonizing agents which affected semi-and final products luminescence properties. The ready nanomaterials were investigated over their chemical structure by FTIR and NMR, whereas morphology was investigated by the TEM method. Their optical properties were determined by UV-VIS spectroscopy. Fluorescence behavior, photo-and pH-stability, as well as solvatochromism showed their applicability in various biomedical applications due to the controlled properties. The samples exhibited excellent antioxidant activity and lack of cytotoxicity on L929 mouse fibroblasts. The results showed that proposed strategy enables preparation of the superior nanomaterials with outstanding luminescence properties such as quantum yield up to 17% which can be successfully applied in cell labelling, bioimaging, and theranostics.Molecules 2020, 25, 736 2 of 18 can release cadmium ions leading to cell death. Importantly, it occurred that quantum dots may undergo certain modifications resulting in the change of their surface or chemical structure [27,28]. Finally, their preparation is expensive. Therefore, there was a need for a novel type of quantum dots which would be suitable for biomedical applications. In 2004, a new type of carbon-based materials was accidentally obtained during nanotube purification [29]. Until then, numerous types of carbon quantum dots (CQDs) have been obtained with various possible applications due to their very attractive properties, such as luminesce, good water solubility, high photostability, resistance to photobleaching or photoblinking, possibility, chemical inertness and, finally, lack of toxicity and biocompability [1][2][3]. Furthermore, CQDs' region of photoluminescence is very broad, starting in the ultraviolet and ending in the near-infrared. Although, until now, the luminescence behavior of carbon nanodots is still not fully clear, it is believed that crucial role plays two factors, namely, that the quantum size is below 10 nm and surface defects which may act as excitation energy traps. Interestingly, CQDs can successfully act as both electron donors and acceptors [2][3][4].CQDs can be prepared from various resources, such as peels (mango, orange, banana, pineapple, onion, seaweed etc.), meat, grains, nuts, or vegetable by-products, which makes them a low-cost material [30,31]. Currently, these nanomaterials are widely applied in drug delivery, gene delivery, biosensing, bioimag...

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Section: Antioxidant Activity Studymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Smart, Tunable CQDs with Antioxidant Properties for Biomedical Applications—Ecofriendly Synthesis and Characterization

et al. 2020

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“…NiO‐NPs induced ROS generation in isolated skin mitochondria (both group) were assayed using the flow cytometry (BD Biosciences; FL‐1 channel). In addition, each evaluation is based on the mean fluorescence intensity of 10 000 counts . Also, we used hydrogen peroxide (H 2 O 2 ) as a positive control group for the ROS evaluation.…”

Section: Methodsmentioning

confidence: 99%

Nickel oxide nanoparticles exert selective toxicity on skin mitochondria and lysosomes isolated from the mouse model of melanoma

Rahimi

Naserzadeh

Mousavi

et al. 2019

J Biochem & Molecular Tox

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Nickel oxide nanoparticles (NiO‐NPs) are progressively used for an immense number of new applications in modern industries sectors. Nevertheless, the toxic impact of NiO‐NPs has not been clearly elucidated on human melanoma cell lines at the cellular and molecular level. Hence, this study was designed to examine the in vitro cytotoxicity potentials of NiO‐NPs on malignant cutaneous melanoma (MCM) mitochondria. Results revealed that NiO‐NPs significantly increased reactive oxygen species level, lipid peroxidation, and mitochondrial membrane potential and decreased succinate dehydrogenase activity, glutathione level, and ATP content on skin mitochondria isolated from the mouse model of melanoma compared with the non‐cancerous mouse skin mitochondria. Our results revealed that NiO‐NPs induced lysosomal membrane labialization on mentioned mitochondria. The current study showed that NiO‐NPs could significantly induce selective cytotoxicity on MCM mitochondria. Therefore, this compound may be considered as a promising candidate for further in vivo and clinical studies to reach a new anti‐MCM drug.

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“…An increase in fluorescence intensity indicates an increase in the generation of ROS due to exposure to inflammation of OA. 31 Mitochondria membrane potential (DC m ) assay. The mitochondria of the knee joints were isolated from all groups.…”

Section: Mitochondrial Functionmentioning

confidence: 99%

Synthesis of N,N′-bis(1,5-dimethyl-2-phenyl-1,2-dihydro-3-oxopyrazol-4-yl) sebacamide that ameliorate osteoarthritis symptoms and improve bone marrow matrix structure and cartilage alterations induced by monoiodoacetate in the rat model: “Suggested potent anti-inflammatory agent against COVID-19”

et al. 2020

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To assess the chondroprotective effect and influence of N, N′-bis(1,5-dimethyl-2-phenyl-1,2-dihydro-3-oxopyrazol-4-yl) sebacamide (dpdo) that was synthesized through the reaction of phenazone with sebacoyl chloride and screened for its biological activity especially as anti-arthritic and anti-inflammatory agent in a monoiodoacetate (MA)-induced experimental osteoarthritis (OA) model. Thirty male albino rats weighing “190–200 g” were divided randomly into three groups (10 each): control, MA-induced OA, and MA-induced OA + dpdo. In MA-induced OA rat, the tumor necrosis factor alpha, interleukin 6, C-reactive protein, rheumatoid factors, reactive oxygen species, as well as all the mitochondrial markers such as mitochondria membrane potential, swelling mitochondria, cytochrome c oxidase (complex IV), and serum oxidative/antioxidant status (malondialdehyde level and activities of myeloperoxidase and xanthine oxidase) are elevated. Also, the activity of succinate dehydrogenase (complex II), levels of ATP, the level of glutathione (GSH), and thiol were markedly diminished in the MA-induced OA group compared to the normal control rats. These findings showed that mitochondrial function is associated with OA pathophysiological alterations and high gene expressions of (IL-6, TNF-a, and IL-1b) and suggests a promising use of dpdo as potential ameliorative agents in the animal model of OA and could act as anti-inflammatory agent in case of severe infection with COVID-19. It is clearly appeared in improving the bone cortex and bone marrow in the treated group with the novel compound in histological and transmission electron microscopic sections which is a very important issue today in fighting severe infections that have significant effects on the blood indices and declining of blood corpuscles like COVID-19, in addition to declining the genotoxicity and inflammation induced by MA in male rats. The novel synthesized compound was highly effective in improving all the above mentioned parameters.

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Biochemical mechanisms of dose-dependent cytotoxicity and ROS-mediated apoptosis induced by lead sulfide/graphene oxide quantum dots for potential bioimaging applications

Cited by 52 publications

References 49 publications

Smart, Tunable CQDs with Antioxidant Properties for Biomedical Applications—Ecofriendly Synthesis and Characterization

Smart, Tunable CQDs with Antioxidant Properties for Biomedical Applications—Ecofriendly Synthesis and Characterization

Nickel oxide nanoparticles exert selective toxicity on skin mitochondria and lysosomes isolated from the mouse model of melanoma

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