Biochemical investigation and biological evaluation of the methanolic extract of the leaves of Nyctanthes arbortristis in vitro

Saha, Repon Kumer; Acharya, Srijan; Shovon, Syed Sohidul Haque; Apu, Apurba Sarker; Roy, Priyanka

doi:10.1016/s2221-1691(12)60449-3

Cited by 3 publications

(1 citation statement)

References 17 publications

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“…The presence of triterpenoids and flavonoids was also reported in previous study (Shah et al, 2012). The characteristic of swimming and the climbing behaviors analyzed by the prediction of the possible neurotransmitters involved as an antidepressant like action of N. arbor-tristis extracts.…”

Section: Discussionsupporting

confidence: 70%

Anti-depressant activity of Nyctanthes arbor-tristis in mice

Gupta¹,

Kashyap²,

Asad

et al. 2016

Bangladesh J Pharmacol

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The present study assesses the protective effect of Nyctanthes arbor-tristis (Nyctaginaceae) extracts and in combination with fluoxetine on stress-induced depression in mice. Leaves were extracted using different solvents (petroleum ether, chloroform and hydroethanol) and administered orally for 14 days. These extracts showed significant improvement in the mobility percentage but among these, hydroethanol extract showed better protective effect from day 1 to 14 in both forced swimming and tail suspension test model. Hydroethanol (100 mg/kg) and chloroform (100 mg/kg) extracts with fluoxetine showed synergistic effect when compared with fluoxetine treated group (10 mg/kg) alone at day 7 and 14. Among monoamine levels only hydroethanol extract (400 mg/kg) restored the 5-HT level near to level of fluoxetine-treated group. Hydroethanol extracts with two higher doses showed significant decrease in glucose and triglycerides levels. Clinically, it may useful as anti-depressant drug. Video Clip:<a href="https://youtube.com/v/EYRuTWpPDqk">Tail suspension test:</a> 5 min 57 sec

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Section: Discussionsupporting

confidence: 70%

Anti-depressant activity of Nyctanthes arbor-tristis in mice

Gupta¹,

Kashyap²,

Asad

et al. 2016

Bangladesh J Pharmacol

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Phytochemical Screening of Nyctanthes arbor-tristis Plant Extracts and Their Antioxidant and Antibacterial Activity Analysis

Gahtori

Tripathi

Chand

et al. 2023

Appl Biochem Biotechnol

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Exploration of anti-Malassezia potential of Nyctanthes arbor-tristis L. and their application to combat the infection caused by Mala s1 a novel allergen

Mishra

Pandey

et al. 2016

BMC Complement Altern Med

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BackgroundMalassezia commensal yeasts along with multitude of antigens have been found to be associated with various skin disorders including Pityriasis versicolor (PV). Amongst them Mala s1, a 37 kDa protein has been proved to be a major allergen reacting with a large panel of sera. However, there exists no therapeutic alternative to combat such problems in form of plant based natural compounds. The purpose of this study is in the first place, to determine the anti-Malassezia activity of Nyctanthes arbor-tristis L. (NAT) ethanolic leaf extract through turbidimetric growth curves, disruption of plasma membrane and secondly, it aims to present in silico validation of its active constituents over Mala s1a novel allergen.MethodsThe antifungal susceptibility 50 % ethanolic extract of NAT was determined by broth microdilution method according to CLSI guidelines. Further MICs and IC50 were determined spectrophotometrically using the software SoftMax® Pro-5 (Molecular Devices, USA). Active constituents mediated disruption of plasma membrane was studied through flowcytometry by permeabilization of fluorescent dye Propidium Iodide (PI). Antioxidant activity of the extract was determined using the DPPH stable radical. Molecular validation of fungal DNA from the extract was observed using PCR amplification. In silico analysis of its active constituents over Mala s1 was performed using HEX software and visualized through Pymol.ResultsThe anti-Malassezia potential of NAT leaf extracts reflected moderate MIC 1.05 μg/μl against M. globosa, while least effective against M. restricta with MIC 1.47 μg/μl. A linear correlation coefficient R2 = 0.866 was obtained in case of M. globosa while minimum was observed in M. restricta with R2 = 0.732. The flow cytometric data reveal ~ 75 % cell death when treated with active constituents β-Sitosterol and Calceolarioside A. The docking confirmations and the interaction energies between Mala s1 and the active constituents (β-Sitosterol and Calceolarioside A) from extracts showed an effective binding which suggests Mala s1 as efficient allergen for site specific targeting.ConclusionsThis study revealed that Nyctanthes arbor-tristis L. (NAT) extracts possess high anti-Malassezia potential which is driven mainly by disruption of plasma membrane. Also in silico validation and molecular modeling studies establishes Mala s1 as a novel allergen that could be a potential target in disease treatment. Our results would also provide a foundation for the development of new therapeutic approach using NAT extract as lead compound with high antioxidant property as an added trait for skin care.

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Biochemical investigation and biological evaluation of the methanolic extract of the leaves of Nyctanthes arbortristis in vitro

Cited by 3 publications

References 17 publications

Anti-depressant activity of <i>Nyctanthes arbor-tristis</i> in mice

Anti-depressant activity of <i>Nyctanthes arbor-tristis</i> in mice

Phytochemical Screening of Nyctanthes arbor-tristis Plant Extracts and Their Antioxidant and Antibacterial Activity Analysis

Exploration of anti-Malassezia potential of Nyctanthes arbor-tristis L. and their application to combat the infection caused by Mala s1 a novel allergen

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