Biochemical integration of blood redox state in captive zebra finches (<i>Taeniopygia guttata</i>)

Costantini, David; Monaghan, Pat; Metcalfe, Neil B.

doi:10.1242/jeb.053496

Cited by 61 publications

(66 citation statements)

References 26 publications

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“…This test detects reactive oxygen metabolites (ROMs) such as hydroperoxides using Fenton's reaction. We followed the directions of the kit, but amended it for smaller sample volumes sensu Costantini et al (2011). In brief, we mixed 4 µl of the sample with 200 µl of kit reagent, incubated it at 37°C for 75 min, and read the absorbance at 505 nm using a Tecan (Switzerland) Infinite M200Pro plate reader.…”

Section: Biochemical Assaysmentioning

confidence: 99%

“…MC435, Diacron International; hereafter OXY), which measures the plasma's ability to oppose the pro-oxidizing action of hypochlorous acid. Also following Costantini et al (2011), we amended the kit's instructions by combining 5 µl of a 1:100 dilution of plasma with 200 µl of the hypochlorous acid-based oxidizing solution, incubating the plate for 10 min at 37°C, adding 5 µl of the chromagen solution, and then measuring absorbance at 505 nm using a Tecan (Switzerland) Infinite M200Pro plate reader. We calculated antioxidant capacity relative to control blanks and the kit calibrator, and report values in mmol l −1 HClO neutralized, with larger values corresponding to a more robust antioxidant barrier.…”

Section: Biochemical Assaysmentioning

confidence: 99%

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Eating increases oxidative damage in a reptile

Butler

Lutz

Fokidis

et al. 2016

Journal of Experimental Biology

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While eating has substantial benefits in terms of both nutrient and energy acquisition, there are physiological costs associated with digesting and metabolizing a meal. Frequently, these costs have been documented in the context of energy expenditure while other physiological costs have been relatively unexplored. Here, we tested whether the seemingly innocuous act of eating affects either systemic pro-oxidant (reactive oxygen metabolite, ROM) levels or antioxidant capacity of corn snakes (Pantherophis guttatus) by collecting plasma during absorptive ( peak increase in metabolic rate due to digestion of a meal) and non-absorptive (baseline) states. When individuals were digesting a meal, there was a minimal increase in antioxidant capacity relative to baseline (4%), but a substantial increase in ROMs (nearly 155%), even when controlling for circulating nutrient levels. We report an oxidative cost of eating that is much greater than that due to long distance flight or mounting an immune response in other taxa. This result demonstrates the importance of investigating non-energetic costs associated with meal processing, and it begs future work to identify the mechanism(s) driving this increase in ROM levels. Because energetic costs associated with eating are taxonomically widespread, identifying the taxonomic breadth of eating-induced ROM increases may provide insights into the interplay between oxidative damage and life history theory.

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Section: Biochemical Assaysmentioning

confidence: 99%

Section: Biochemical Assaysmentioning

confidence: 99%

Eating increases oxidative damage in a reptile

Butler

Lutz

Fokidis

et al. 2016

Journal of Experimental Biology

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“…The manufacturer's instructions were followed with one addition: samples were centrifuged immediately after incubation (75 min at 378C) [18]. Measured values were calibrated with a reference standard, and converted to millimolar of H 2 O 2 equivalents.…”

Section: (B) Physiological Measurementsmentioning

confidence: 99%

Female plumage colour influences seasonal oxidative damage and testosterone profiles in a songbird

2013

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Across diverse taxa, morphological traits mediate social interactions and mate selection. Physiological constraints on signal elaboration have been widely documented, but the potential for trait display to influence physiological state remains poorly understood. We tested for the presence of causal links between ventral plumage colour-a trait known to covary with reproductive performance-and physiological measures in female North American barn swallows, Hirundo rustica erythrogaster. Naturally darker swallows have lower levels of plasma oxidative damage. Females manipulated to display darker ventral plumage during reproduction rapidly decreased oxidative damage, adopting the physiological state of naturally darker individuals. These results support the presence of a social mechanism that links static plumage traits with the physiological state of their bearer during trait advertisement, long after the completion of signal development.

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“…Some avian studies report evidence of correlations among OS markers (e.g., in captive zebra finches ( Taeniopygia guttata ), Costantini et al. 2011), whereas others did not detect any such correlations (e.g., in wild‐caught captive greenfinches ( Carduelis chloris), Sepp et al. 2012).…”

Section: Introductionmentioning

confidence: 95%

Plasma markers of oxidative stress are uncorrelated in a wild mammal

Christensen

Selman

Blount

et al. 2015

Ecology and Evolution

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Oxidative stress, which results from an imbalance between the production of potentially damaging reactive oxygen species versus antioxidant defenses and repair mechanisms, has been proposed as an important mediator of life‐history trade‐offs. A plethora of biomarkers associated with oxidative stress exist, but few ecological studies have examined the relationships among different markers in organisms experiencing natural conditions or tested whether those relationships are stable across different environments and demographic groups. It is therefore not clear to what extent studies of different markers can be compared, or whether studies that focus on a single marker can draw general conclusions regarding oxidative stress. We measured widely used markers of oxidative damage (protein carbonyls and malondialdehyde) and antioxidant defense (superoxide dismutase and total antioxidant capacity) from 706 plasma samples collected over a 4‐year period in a wild population of Soay sheep on St Kilda. We quantified the correlation structure among these four markers across the entire sample set and also within separate years, age groups (lambs and adults), and sexes. We found some moderately strong correlations between some pairs of markers when data from all 4 years were pooled. However, these correlations were caused by considerable among‐year variation in mean marker values; correlation coefficients were small and not significantly different from zero after accounting for among‐year variation. Furthermore, within each year, age, and sex subgroup, the pairwise correlation coefficients among the four markers were weak, nonsignificant, and distributed around zero. In addition, principal component analysis confirmed that the four markers represented four independent axes of variation. Our results suggest that plasma markers of oxidative stress may vary dramatically among years, presumably due to environmental conditions, and that this variation can induce population‐level correlations among markers even in the absence of any correlations within contemporaneous subgroups. The absence of any consistent correlations within years or demographic subgroups implies that care must be taken when generalizing from observed relationships with oxidative stress markers, as each marker may reflect different and potentially uncoupled biochemical processes.

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Biochemical integration of blood redox state in captive zebra finches (Taeniopygia guttata)

Cited by 61 publications

References 26 publications

Eating increases oxidative damage in a reptile

Eating increases oxidative damage in a reptile

Female plumage colour influences seasonal oxidative damage and testosterone profiles in a songbird

Plasma markers of oxidative stress are uncorrelated in a wild mammal

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