Biochemical indicators of contaminant exposure in spotted pigfish (Orthopristis ruber) caught at three bays of Rio de Janeiro coast

Ventura, Eliana Cristina; Gaelzer, Luiz Ricardo; Zanette, Juliano; Marques, Maria Risoleta Freire; Bainy, Afonso Celso Dias

doi:10.1016/s0141-1136(02)00137-x

Cited by 39 publications

(13 citation statements)

References 7 publications

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“…The GST levels had an increase in response to the pronounced oxidative stress in contaminated areas [22]. On the other hand, low levels of GST activity can also be found in highly contaminated areas [23]. Both these conditions were observed during this study, in which only in the contaminated area were the highest values (2.82 μmol/min/mg of protein) and the lowest values (0.31 μmol/min/mg of protein).…”

Section: Discussionsupporting

confidence: 58%

Biomarkers in Catfish Sciades herzbergii (Teleostei: Ariidae) from Polluted and Non-polluted Areas (São Marcos’ Bay, Northeastern Brazil)

Neta

Torres

Abreu-Silva

2012

Appl Biochem Biotechnol

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Biomarkers based on specific enzyme activities and histological alterations are useful tools for evaluating toxicological effects of xenobiotics in wild fish. In this work, an experimental system of biomarkers with enzyme glutathione S-transferase (GST) and branchial lesions in catfish (Sciades herzbergii) was mathematically modeled. The fish were collected along known pollution gradients (S1) and from areas regarded relatively free of anthropogenic input (S2) in São Marcos' Bay, Brazil. GST was measured spectrophotometrically, and branchial lesions were examined by light microscope. The databases from this analysis were compiled, and non-linear models were used to analyze the dependence of the enzyme activity on the areas of sampling and on selected biological parameters of the fish. Fish weight, length, and somatic indices (gonadosomatic index) were significant in the model of GST activity only in A2. Brachial lesions were significant in the model of GST activity only in A1. The obtained model indicates that when the GST ceases to act, serious branchial lesions are observed in the fish of the contaminated regions.

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Section: Discussionsupporting

confidence: 58%

Biomarkers in Catfish Sciades herzbergii (Teleostei: Ariidae) from Polluted and Non-polluted Areas (São Marcos’ Bay, Northeastern Brazil)

Neta

Torres

Abreu-Silva

2012

Appl Biochem Biotechnol

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“…A similar study carried out by Ventura et al (2002) showed that higher CAT activity in fish from Guanabara Bay, might be related to the presence of greater amounts of peroxisome proliferators at that site. Glutathione S-transferase activity in the livers of Sciades herzbergii showed a significant response to oxidative stress at the contaminated site in São Marcos' Bay, possibly related to a higher level of contamination.…”

Section: Discussionsupporting

confidence: 51%

“…Comparative studies of biochemical indicators of contaminant exposure in spotted pigfish (Orthopristis ruber) caught in three bays on the Rio de Janeiro coast showed that biometric data (total length, weight) in those fishes could be a factor affecting the biochemical analyses such as the activity of the antioxidant defence catalase and the phase II biotransformation enzyme glutathione S-transferase (Ventura et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Sciades herzbergii oxidative stress biomarkers: an in situ study of an estuarine ecosystem (São Marcos' Bay, Maranhão, Brazil)

Carvalho-Neta

Abreu-Silva

2010

Braz. j. oceanogr.

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A B S T R A C TIn order to study the effects of environmental contamination on wild fish, sites were sampled in São Marcos' Bay. The first is located near the ALUMAR/ALCOA port, a potentially contaminated area. The second, located near the Coqueiro beach, was used as a reference area. The activity of antioxidant defence catalase (CAT) and glutathione S-transferase (GST) in S. herzbergii was compared with the biometric data and gonadosomatic index (GSI). The result showed that GSI decreased significantly in females (p<0.05) at the contaminated site. The activity of CAT was higher in fish caught at the contaminated site. A significant difference was observed in GST activity in the liver of S. herzbergii in the comparison between fish from the contaminated site and those from the reference site (p<0.05). GSI provides new insights into the nature of the detoxification response in this catfish species because it is not correlated with the enzymes at the potentially contaminated site. We are, therefore, in a position to suggest that the elevated GST/CAT activity might well be related to the reproduction of the animals at the reference site but not at the potentially contaminated site. If that be the case, one may conclude that GST/CAT and GSI are useful biomarkers of aquatic contamination. R E S U M OO objetivo desse trabalho foi estudar os efeitos da contaminação ambiental em peixes amostrados em dois locais da Baía de São Marcos. O primeiro ponto está localizado próximo ao porto da ALUMAR/ALCOA, considerado como uma área potencialmente contaminada. O segundo ponto, situado na praia do Coqueiro, foi usado como uma área de referência. Dados da atividade da enzima de defesa antioxidante catalase (CAT) e da glutationa S-transferase (GST) em S. herzbergii foram comparados com os dados biométricos e o índice gonadossomático (GSI). Resultados mostraram que o GSI diminuiu significativamente em fêmeas (p <0.05) no local contaminado. A atividade da CAT foi mais alta nos peixes do local contaminado. Uma diferença significativa foi observada na atividade de GST de S. herzbergii no local contaminado e no local de referência (p <0.05). GSI possibilitou uma nova abordagem quanto à natureza da resposta de destoxificação nessa espécie de bagre porque este índice não apresentou correlação com as enzimas no local potencialmente contaminado, mas apresentou no local de referência. Assim, sugere-se que a boa correlação da GST/CAT e GSI poderia estar relacionada à reprodução dos animais no local de referência, mas não no local potencialmente contaminado. Se esse for o caso, pode-se concluir que GST/CAT e GSI podem ser utilizados como bons biomarcadores para avaliar contaminação aquática.

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“…Shifting and depletion of the reduced glutathione pool to oxidized glutathione through the oxidation protection pathway could limit the amount of reduced glutathione available to GST for conjugation of xenobiotics (Ventura et al 2002). Although the method of enzyme preparation was different, hepatic GST activity variation present in detoxification defense responses to xenobiotics across tissues and species would be another explanation (Hasspieler et al 1994;Sorsa et al 2000a).…”

Section: Discussionmentioning

confidence: 99%

Acute toxicity of the pesticide methomyl on the topmouth gudgeon (Pseudorasbora parva): mortality and effects on four biomarkers

Jiang

Gao

et al. 2007

Fish Physiol Biochem

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In this study, the acute toxicity of the pesticide methomyl on the topmouth gudgeon (Pseudorasbora parva) was evaluated using mortality and the activity of the enzymes acetylcholinesterase (AChE), glutathione S-transferases (GSTs), glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) as endpoints. LC50 values were 1.228, 0.782, 0.538, and 0.425 mg/l at 24, 48, 72, and 96 h of exposure, respectively. Methomyl caused a sharp decrease in specific activity of brain AChE around 48% at concentrations between 0.043 and 0.213 mg/l. A reduction higher than 40% in liver GST activity at concentrations between 0.085 and 0.213 mg/l was found, whereas no significant effects were observed in intestinal GST. A significant concentration-dependent decrease of GOT activity was found after 24 h of exposure to the pesticide but not after 96 h. No significant effects on GPT activity were observed. These results indicate that at the concentrations tested, methomyl is acutely toxic to the species P. parva, causing mortality, neurotoxic effects, and changes in some hepatic enzymes.

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Biochemical indicators of contaminant exposure in spotted pigfish (Orthopristis ruber) caught at three bays of Rio de Janeiro coast

Cited by 39 publications

References 7 publications

Biomarkers in Catfish Sciades herzbergii (Teleostei: Ariidae) from Polluted and Non-polluted Areas (São Marcos’ Bay, Northeastern Brazil)

Biomarkers in Catfish Sciades herzbergii (Teleostei: Ariidae) from Polluted and Non-polluted Areas (São Marcos’ Bay, Northeastern Brazil)

Sciades herzbergii oxidative stress biomarkers: an in situ study of an estuarine ecosystem (São Marcos' Bay, Maranhão, Brazil)

Acute toxicity of the pesticide methomyl on the topmouth gudgeon (Pseudorasbora parva): mortality and effects on four biomarkers

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