Biochemical imaging below the diffraction limit – probing cellular membrane related structures by tip‐enhanced Raman spectroscopy (TERS)

Böhme, René; Cialla, Dana; Richter, Marc; Rösch, Petra; Popp, Jürgen; Deckert, Volker

doi:10.1002/jbio.201000030

Cited by 77 publications

(61 citation statements)

References 29 publications

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“…The Raman bands at 1091, 1302, 1450, and 1660 cm −1 correspond to C─C stretching vibration, amide Ш, CH deformation, and amide І, respectively (Table 1). 21,22 These bands are mainly derived from the lipid and protein contents. The bands at 2853 and 2885 cm −1 arise from symmetric and asymmetric CH 2 stretching modes.…”

Section: Raman Spectra Of Intact and Injured Sciatic Nervesmentioning

confidence: 99%

Application of Raman spectroscopy for visualizing biochemical changes during peripheral nerve injuryin vitroandin vivo

et al. 2013

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Abstract. Raman spectroscopy can be used for analysis of objects by detecting the vibrational spectrum using labelfree methods. This imaging method was applied to analysis of peripheral nerve regeneration by examining the sciatic nerve in vitro and in vivo. Raman spectra of intact nerve tissue had three particularly important peaks in the range 2800 − 3000 cm −1 . Spectra of injured sciatic nerves showed significant changes in the ratio of these peaks. Analysis of cellular spectra suggested that the spectrum for sciatic nerve tissue reflects the axon and myelin components of this tissue. Immunohistochemical analysis showed that the number of axons and the myelinated area were reduced at 7 days after injury and then increased by 28 days. The relative change in the axon to myelin ratio showed a similar initial increase, followed by a decrease at 28 days after injury. These changes correlated with the band intensity ratio and the changes in distribution of axon and myelin in Raman spectral analysis. Thus, our results suggest that label-free biochemical imaging with Raman spectroscopy can be used to detect turnover of axon and myelin in peripheral nerve regeneration. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Section: Raman Spectra Of Intact and Injured Sciatic Nervesmentioning

confidence: 99%

Application of Raman spectroscopy for visualizing biochemical changes during peripheral nerve injuryin vitroandin vivo

et al. 2013

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“…The past decade has seen significant development of both modalities for molecularly specific analysis on the micron and even nanoscale [11][12][13][14][15][16][17] . Analysis of bodily fluids for diagnosis and health monitoring presents a less invasive approach than performing biopsies of organs for example during cancer screening 18 .…”

Section: Introductionmentioning

confidence: 99%

Vibrational spectroscopic analysis of body fluids: avoiding molecular contamination using centrifugal filtration

2014

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“…Phase separated domains of deuterated saturated lipids and protonated unsaturated lipids were imaged [148]. A streptavidin containing phospholipid film showed spectral differences attributed to putative domains [149]. A fundamental challenge to studying membranes is TERS in solution has been difficult [115].…”

Section: Biomolecules and Cellsmentioning

confidence: 99%

Tip enhanced Raman scattering: plasmonic enhancements for nanoscale chemical analysis

2014

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Tip enhanced Raman scattering (TERS) is an emerging technique that uses a metalized scanning probe microscope tip to spatially localize electric fields that enhances Raman scattering enabling chemical imaging on nanometer dimensions. Arising from the same principles as surface enhanced Raman scattering (SERS), TERS offers unique advantages associated with controling the size, shape, and location of the enhancing nanostructure. In this article we discuss the correlations between current understanding of SERS and how this relates to TERS, as well as how TERS provides new understanding and insights. The relationship between plasmon resonances and Raman enhancements is emphasized as the key to obtaining optimal TERS results. Applications of TERS, including chemical analysis of carbon nanotubes, organic molecules, inorganic crystals, nucleic acids, proteins, cells and organisms, are used to illustrate the information that can be gained. Under ideal conditions TERS is capable of single molecule sensitivity and sub-nanometer spatial resolution. The ability to control plasmonic enhancements for chemical analysis suggests new experiments and opportunities to understand molecular composition and interactions on the nanoscale.

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Biochemical imaging below the diffraction limit – probing cellular membrane related structures by tip‐enhanced Raman spectroscopy (TERS)

Cited by 77 publications

References 29 publications

Application of Raman spectroscopy for visualizing biochemical changes during peripheral nerve injuryin vitroandin vivo

Application of Raman spectroscopy for visualizing biochemical changes during peripheral nerve injuryin vitroandin vivo

Vibrational spectroscopic analysis of body fluids: avoiding molecular contamination using centrifugal filtration

Tip enhanced Raman scattering: plasmonic enhancements for nanoscale chemical analysis

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