Biochemical identification of Aeromonas genospecies isolated from humans

Altwegg, Martin; Steigerwalt, A G; Altwegg-Bissig, Regula; Lüthy-Hottenstein, J; Brenner, Don J.

doi:10.1128/jcm.28.2.258-264.1990

Cited by 145 publications

(84 citation statements)

References 21 publications

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“…caviae (HG4) and Aer. veronii biovar sobria (HG8) are the most common species associated with human intestinal disease (Kuijper et al 1989;Altwegg et al 1990;Havelaar et al 1992;Khün et al 1997;Borrell et al 1998).…”

Section: Introductionmentioning

confidence: 99%

Virulence markers in Aeromonas hydrophila and Aeromonas veronii biovar sobria isolates from freshwater fish and from a diarrhoea case

et al. 2002

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Aims: To evaluate the public health significance of representative strains of two Aeromonas spp., mainly from freshwater fish, on the basis of production of virulence-associated factors and presence of the haemolytic genes aerA and hlyA. Methods and Results: Eleven strains of Aer. hydrophila, three strains of Aer. veronii biovar sobria (all from freshwater fish) and one strain of Aer. hydrophila from human diarrhoea were tested for potential virulence traits and for the presence of the haemolytic genes aerA and hlyA. Ten Aer. hydrophila isolates were aerA + hlyA + and two aerA + hlyA -. Aeromonas veronii biovar sobria isolates were aerA -hlyA -. Strains from the three genotypes showed enterotoxic activity in the suckling mouse assay. At 28°C, four Aer. hydrophila fish strains could be considered as potentially virulent (possessing at least two of these characteristics: haemolytic, cytotoxic and enterotoxic). One Aer. veronii biovar sobria strain and the clinical isolate were cytotoxic on Vero cells. When grown at 4°C, these six isolates fulfilled virulence criterion, but at 37°C, only one fish strain, an Aer. hydrophila, did. Conclusions: The potential health risk derived from the presence of Aer. hydrophila and Aer. veronii biovar sobria in ice-stored freshwater fish should not be underestimated. Significance and Impact of the Study: Expression of virulence factors is affected by temperature incubation and not always related to the presence of haemolytic genes.

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Section: Introductionmentioning

confidence: 99%

Virulence markers in Aeromonas hydrophila and Aeromonas veronii biovar sobria isolates from freshwater fish and from a diarrhoea case

et al. 2002

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“…encheleia HG16 and Aerom. popoffii HG17 (Allen et al 1983;Farmer et al 1986;Hickman-Brenner et al 1987;Hickman-Brenner et al 1988;Schubert and Hegazi 1988;Kuijper et al 1989;Altwegg et al 1990;Carnahan et al 1991a;Carnahan et al 1991b;Carnahan et al 1991c;Martínez-Murcia et al 1992;Esteve et al 1995;Ali et al 1996;Huys et al 1997).…”

Section: Introductionmentioning

confidence: 99%

“…caviae and Aerom. sobria complex (Janda and Abbott 1998) because of the large number of phenotypic tests required for an accurate biochemical identification of all genospecies (Altwegg et al 1990;Abbott et al 1992;Kämpfer and Altwegg 1992;Janda et al 1996;Oakey et al 1996;Carson et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Biochemical identification and numerical taxonomy of Aeromonas spp. isolated from environmental and clinical samples in Spain

et al. 2002

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Aims: To study the phenotypic characteristics of Aeromonas spp. from environmental and clinical samples in Spain and to cluster these strains by numerical taxonomy. Methods and Results: A collection of 202 Aeromonas strains isolated from bivalve molluscs, water and clinical samples was tested for 64 phenotypic properties; 91% of these isolates were identified at species level. Aeromonas caviae was predominant in bivalve molluscs and Aerom. bestiarum in freshwater samples. Cluster analyses revealed eight different phena: three containing more than one DNA-DNA hybridization group but including strains that belong to the same phenospecies complex (Aerom. hydrophila, Aerom. sobria and Aerom. caviae), Aerom. encheleia, Aerom. trota and three containing unidentified Aeromonas strains isolated from bivalve molluscs. Conclusions: Aeromonas spp. are widely distributed in environmental and clinical sources. A selection of 16 of the phenotypical tests chosen allowed the identification of most isolates (91%), although some strains remain unidentified, mainly isolates from bivalve molluscs, suggesting the presence of new Aeromonas species. Numerical taxonomy was not in total concordance with the identification of the studied strains. Significance and Impact of the Study: Numerical taxonomy of Aeromonas strains isolated from different sources revealed the presence of potentially pathogenic Aeromonas spp., especially in bivalve molluscs, and phena with unidentified strains that suggest new Aeromonas species.

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“…The taxonomy of Aeromonas spp. has been revised recently and the genus now includes ten species (A. hydrophila, A. sobria, A. caviae, A. salmonicida, A. media, A. veronii, A. schubertii, A. eucrenophilu, A. jandaei and A. trotu) (Carnahan et al, 1991), most of which have been associated with disease in humans (Altwegg et al, 1990). These micro-organisms are capable of producing a wide range of toxins, which is compatible with the scope of gastrointestinal and extraintestinal illness which Aeromonas infections can cause (Rolston, 1988).…”

Section: Clostridium Difficilementioning

confidence: 99%