Biochemical, haematological and oxidative stress responses of common carp (Cyprinus carpio L.) after sub-chronic exposure to copper

Ševčíková, Marie; Modrá, Helena; Blahová, Jana; Dobšíková, Radka; Plhalová, Lucie; Zítka, Ondřej; Hynek, David; Kizek, René; Škorič, M.; Svobodová, Zdeňka

doi:10.17221/8681-vetmed

Cited by 38 publications

(18 citation statements)

References 81 publications

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“…Among metal ligands, induced MTs are involved in non-enzymatic homeostasis and a detoxifi cation mechanism of fi sh against metal exposure. In previous studies, the induction of MT content in fi sh gills exposed to metals has been documented in tilapia (Oreochromis mossambicus) [43][44], common carp (Cyprinus carpio) [45][46], and catfi sh (Clarias gariepinus) [47]. Our study also demonstrated that waterborne exposure to Cu 2+ could increase MT contents in gills of C. auratus in a concentration-dependent manner, confi rming that MT synthesis level in C. auratus gills is a sensitive biomarker for aquatic pollution of metals.…”

Section: Discussion Of Resultsmentioning

confidence: 96%

Interactive Effects of Copper, Fluorene, and Fluoranthene on Enzymatic Biomarkers and Metallothionein Levels in Crucian Carp (Carassius auratus)

Lu¹,

Ding

2018

Pol. J. Environ. Stud.

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To evaluate potential interactive effects of metals and polycyclic aromatic hydrocarbons (PAHs) on biomarker responses, groups of the freshwater fi sh crucian carp (Carassius auratus) were exposed to single and binary combinations of copper (Cu) (0.01-0.16 mg L-1) with fl uorene (Fl) or fl uoranthene (Fluo) (2-10 mg kg -1 ) for 96 h. Dose-dependent increases in the activities of phases I and II metabolic enzymes [7-ethoxyresorufi n O-deethylase (EROD) and glutathione-S-transferase (GST), respectively] were observed in fi sh liver exposed to Fl and Fluo, but these enzyme activities did not differ signifi cantly from the controls when co-treated with higher concentrations of Cu, suggesting an inhibiting interaction on the metabolic enzymes. Although Cu did not alter catalase (CAT) activity, CAT activity was decreased in fi sh liver exposed to the two PAHs alone or in combination with Cu. Although metallothionein (MT) content in gills was signifi cantly increased following exposure to Cu alone or in combination with Fl and Fluo, the induction folds of MT decreased under co-exposure. Co-exposures to these chemicals invoked complex biomarker responses in fi sh liver and gills. These results highlight the need for careful consideration of the interactive effects of multiple environmental stressors on fi sh.

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Section: Discussion Of Resultsmentioning

confidence: 96%

Interactive Effects of Copper, Fluorene, and Fluoranthene on Enzymatic Biomarkers and Metallothionein Levels in Crucian Carp (Carassius auratus)

Lu¹,

Ding

2018

Pol. J. Environ. Stud.

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“…Abarghoei et al [32] treated the gold fish to silver sulphate, the lymphocyte count decreased at 24 and 48 hours exposure period and increased in 96 hours, but the results of monocytes counts showed significant similarities. Sevcikova et al [33]in the blood of common carp (Cyprinuscarpio)and Hedayati and Darabitabar [34] in Caspian roach (Rutilusrutilus) observed significantly greater lymphocyte reduction at the highest concentration of copper whereas monocyte and neutrophil counts increased as compared to the control group. The present studies indicate that exposure to lead alters the leucocyte counts in fish, H. fossilis when exposed to lead.…”

Section: Discussionmentioning

confidence: 99%

Haemotoxic Assessment of Differential Leucocyte Counts (DLC) in Fresh Water Teleost, Heteropneustes fossilis Exposed to Lead

Shalaby

Gupta

Verma³

et al. 2020

NIDOC-ASRT

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discharged into rivers, through drainage (domestic, industry and agriculture) which can accumulate and biomagnify along water, sediment, and the aquatic food chain, resulting in sublethal effects or death in local fish populations. Mastan et al.[4] reported the haematological variables are extensively being used in clinical diagnosis of fish physiology in order to determine the effects of external stressors and diseased conditions in fish. Haematological abnormalities have also been studied undersublethal concentration of pollutants such as heavy metals exposed to fish: H. fossilis B ACKGROUND, in recent years, fish blood is being used for assessing the health and general condition of the fish subject to changing environmental conditions due to heavy metals which are regularly released by anthropogenic activities. The present experiment was set up to assess the effects of the heavy metal, lead (Pb) on the Differential Leucocyte Counts (DLC) of fresh water teleost Heteropneustes fossilis. Results.The acclimatized fish were exposed to different concentrations of lead [2.25ppm (Group B), 2.65ppm (Group C), and 2.85ppm (Group D)] and the values were compared with the control(Group A). The time interval of examination was 7, 14, 21 and 28 days post exposure. DLC showed changes in small lymphocytes resulting in the fall of 41% to 31% at 2.65 ppm of Pb during 28 days of exposure period. The large lymphocyte counts also decreased from normal value of 39% to 27% at maximum concentration and maximum exposure period (2.85 ppm Pb; 28 days). The neutrophils increased from normal counts (14%) to maximum (26%) at 2.65 ppm of Pb during 28 days exposure period. The monocyte counts increased from normal counts of 3% to 7% during 7 and 28 days exposure period at 2.65 ppm of Pb. The counts of eosinophils increased against the toxicity of lead from 2% to 6% at maximum concentration (2.85 ppm) during 14 and 28 days exposure period. Basophils increased from normal range (1%) to 3% at 2.65 ppm of Pb at 28 days exposure period. Conclusion, our results indicated that exposure to lead results in altered leucocyte counts.

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“…Selected liver tissues were prepared for histopathology. Tissues were fixed in buffered 10% neutral formalin, dehydrated, embedded in paraffin and sectioned on a microtome at thickness of 4 μm and stained with hematoxylin and eosin [26,44].…”

Section: Methodsmentioning

confidence: 99%

FTIR study of the binary effect of titanium dioxide nanoparticles (nTiO2) and copper (Cu2+) on the biochemical constituents of liver tissues of catfish (Clarias gariepinus)

Biochemical, haematological and oxidative stress responses of common carp (Cyprinus carpio L.) after sub-chronic exposure to copper

Cited by 38 publications

References 81 publications

Interactive Effects of Copper, Fluorene, and Fluoranthene on Enzymatic Biomarkers and Metallothionein Levels in Crucian Carp (Carassius auratus)

Interactive Effects of Copper, Fluorene, and Fluoranthene on Enzymatic Biomarkers and Metallothionein Levels in Crucian Carp (Carassius auratus)

Haemotoxic Assessment of Differential Leucocyte Counts (DLC) in Fresh Water Teleost, Heteropneustes fossilis Exposed to Lead

FTIR study of the binary effect of titanium dioxide nanoparticles (nTiO2) and copper (Cu2+) on the biochemical constituents of liver tissues of catfish (Clarias gariepinus)

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