Biochemical, functional and molecular characterization of pigeon pea rhizobia isolated from semi-arid regions of India

Jain, Devendra; Kumari, Anisha; Saheewala, Heena; Sanadhya, Suman; Maheshwari, D. K.; Meena, R. H.; Singh, Abhijeet; Gera, Rajesh; Mohanty, Santosh Ranjan

doi:10.1007/s00203-020-01904-0

Cited by 10 publications

(5 citation statements)

References 30 publications

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“…According to Bergey's Manual of Systematic Bacteriology and previous research methods, further biochemical characterization of strain HK9-3 was performed, and physiological and morphological characteristics of strain HK9-3 were compared with the original strain S. plymuthica 6109D01 (ATCC No. 53858) (Bergey, 1994;Jain et al, 2020). Two strains of S. plymuthica tested in this experiment showed completely identical characteristics to the authentic strain (Table S1).…”

Section: Identification Of S Plymuthica Hk9-3supporting

confidence: 51%

Serratia plymuthicaHK9‐3 enhances tomato resistance against Phytophthora capsici by modulating antioxidant defense systems and rhizosphere micro‐ecological condition

Wang,

Piao,

et al. 2024

Physiologia Plantarum

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Tomatoes are frequently challenged by various pathogens, among which Phytophthora capsici (P. capsici) is a destructive soil‐borne pathogen that seriously threatens the safe production of tomatoes. Plant growth‐promoting rhizobacteria (PGPR) positively induced plant resistance against multiple pathogens. However, little is known about the role and regulatory mechanism of PGPR in tomato resistance to P. capsici. Here, we identified a new strain Serratia plymuthica (S. plymuthica), HK9‐3, which has a significant antibacterial effect on P. capsici infection. Meanwhile, stable colonization in roots by HK9‐3, even under P. capsici infection, improved tomato growth parameters, root system architecture, photosynthetic capacity, and boosted biomass. Importantly, HK9‐3 colonization significantly alleviated the damage caused by P. capsici infection through enhancing ROS scavenger ability and inducing antioxidant defense system and pathogenesis‐related (PR) proteins in leaves, as evidenced by elevating the activities of peroxidase (POD), superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), phenylalanine ammonia lyase (PAL), polyphenol oxidase (PPO), and chitinase, β‐1,3‐glucanase, and increasing the transcripts of POD, SOD, CAT, APX1, PAL1, PAL2, PAL5, PPO2, CHI17 and β‐1,3‐glucanase genes. Notably, HK9‐3 colonization not only effectively improved soil microecology and soil fertility, but also significantly enhanced fruit yield by 44.6% and improved quality. Our study presents HK9‐3 as a promising and effective solution for controlling P. capsici infection in tomato cultivation while simultaneously promoting plant growth and increasing yield, which may have implications for P. capsici control in vegetable production.

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Section: Identification Of S Plymuthica Hk9-3supporting

confidence: 51%

Serratia plymuthicaHK9‐3 enhances tomato resistance against Phytophthora capsici by modulating antioxidant defense systems and rhizosphere micro‐ecological condition

Wang,

Piao,

et al. 2024

Physiologia Plantarum

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“…This could be associated with the fact that diazotrophs can develop in free life before colonizing leguminous species that can provide the conditions to x N and that the samples analyzed are not rhizospheric soil. Diazotrophs such as Mesorhizobium, Rhizobium, and Ensifer predominate in nodules of leguminous plants with the potential to promote plant growth under the limited conditions of arid and semi-arid zones [55]. Azospira reduces N sources such as nitrate [56] and N 2 O under microaerobic or anaerobic conditions from easily degradable substrates such as acetate [36].…”

Section: Nitrogen Metabolismmentioning

confidence: 99%

Soil microbial communities from fertile islands under semi-arid conditions in La Guajira, Colombian Caribbean

Leal¹,

Franco²,

Vanegas

2022

Preprint

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Fertile islands are key ecological arrangements for slowing desertification processes in arid and semi-arid environments. These islands harbor a great variety of microorganisms that play an important role in their establishment and dynamics. The taxonomic and functional diversity of bacteria on these islands is conditioned by the nurse tree and the characteristics of their environment. However, the microbial diversity associated with these vegetation patterns is not described in the semi-arid environments of northern South America. The present study analyzed the taxonomic and functional diversity of soil microbial communities of fertile islands belonging to three species of nurse trees in the Colombian Caribbean during a wet and a dry season. DNA extraction and massive sequencing were performed from soil surface samples under the nurse plant canopy and bare soil. The SILVA database was used for taxonomic analyses, DIAMOND and the KEGG database for functional analyses, and STAMP for statistical differences. Taxonomic and functional diversity was mainly determined by seasonality and the presence or absence of vegetation. No significant differences were found among nurse plant species. The highest taxonomic abundances were recorded in the dry season under the nurse tree for the phyla Proteobacteria and Actinobacteriota, and the genera Sphingomonas, Azospira, Solirubrobacter, Flavisolibacter, and Geodermatophilus. While the highest functional potential was detected in the wet season. The presence of several biogeochemical cycling modules and unique genera suggest the presence of microniches that could be differentiated by oxygen tension and nutrient availability due to seasonality and the presence or absence of vegetation.

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“…(14) reported that members of the family Burkholderi-aceae, class β-proteobacteria, called β-rhizobia, also nodulate legumes. Furthermore, it was recorded that rhizobial bacteria have been well develop and was examined and detected not to be the only microorganism isolated from the root of nodules of legumes (15). (5) reported that non-rhizobial bacteria isolated from the nodules of legumes are primarily Bacillus, Enterobacter, Agrobacterium, Acinetobacter, Paenibacillus, Pantoea, Mycobacterium, Micromonospora and Pseudomonas.…”

Section: Introductionmentioning

confidence: 99%

Isolation and molecular characterization of root nodules bacteria on inoculated Bambara groundnut (Vigna subterranea (L) Verdc) Accessions

Bitire

Abberton²,

Oyatomi³

et al. 2023

Preprint

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Soils in the tropics have often shown diverse reduction in the population of bacteria due to the pressure on the agricultural system with the nature of the climatic conditions, which eventually affect soil health. High priority has been placed on rhizobia inoculants over the inorganic fertilizer by farmers and this has led to the onset of great research on tropical rhizobia strains. In this study, bacteria isolates from root nodules of inoculated Bambara groundnut (BGN) were characterized using 16S rRNA gene, nitrogen fixing gene (nifH) and nodulating gene (nod A and nodC ). The result of the 16S rRNA revealed that the root of BGN are mostly associated with Bradyrhizobium spp (B. spp), which include: B. diazoefficiens, B. japonicum and Rhizobium spp in the screenhouse. The nifH, nod A and nod C gene analysis revealed that Streptomyces bacillaris, Pseudomonas knackmussi, B. ekanii, Sinorhizobium meliloti, Mesorhizobium spp, Bradyrhizobium spp and Rhizobium spp were isolated from the field in two locations (Ibadan and Ikenne). The rhizobia isolates have been previously confirmed to be playing an important role in nodulation and nitrogen fixation of BGN. The result of the study, therefore, revealed the isolation of bacteria species from the root nodules of BGN accessions using molecular techniques.

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Biochemical, functional and molecular characterization of pigeon pea rhizobia isolated from semi-arid regions of India

Cited by 10 publications

References 30 publications

Serratia plymuthicaHK9‐3 enhances tomato resistance against Phytophthora capsici by modulating antioxidant defense systems and rhizosphere micro‐ecological condition

Serratia plymuthicaHK9‐3 enhances tomato resistance against Phytophthora capsici by modulating antioxidant defense systems and rhizosphere micro‐ecological condition

Soil microbial communities from fertile islands under semi-arid conditions in La Guajira, Colombian Caribbean

Isolation and molecular characterization of root nodules bacteria on inoculated Bambara groundnut (Vigna subterranea (L) Verdc) Accessions

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