Biochemical features and early adhesion of marine Candida parapsilosis strains on high-density polyethylene

Abstract

Aims: Plastic debris are constantly released into oceans where, due to weathering processes, they suffer fragmentation into micro-and nanoplastics. Diverse microbes often colonize these persisting fragments, contributing to their degradation.However, there are scarce reports regarding the biofilm formation of eukaryotic decomposing microorganisms on plastics. Here, we evaluated five yeast isolates from deep-sea sediment for catabolic properties and early adhesion ability on high-density polyethylene (HDPE). Methods and Results:We assessed yeast catabolic features and adhesion ability on HDPE fragments subjected to abiotic weathering. Adhered cells were evaluated through Crystal Violet Assay, Scanning Electron Microscopy, Atomic Force Microscopy and Infrared Spectroscopy. Isolates were identified as Candida parapsilosis and exhibited wide catabolic capacity. Two isolates showed high adhesion ability on HDPE, consistently higher than the reference C. parapsilosis strain, despite an increase in fragment roughness due to weathering. Isolate Y5 displayed the most efficient colonization, with production of polysaccharides and lipids after 48 h of incubation. Conclusion:This work provides insights on catabolic metabolism and initial yeast-HDPE interactions of marine C. parapsilosis strains.Significance and Impact of the Study: Our findings represent an essential contribution to the characterization of early interactions between deep-sea undescribed yeast strains and plastic pollutants found in oceans.

Environmental reservoirs of the drug-resistant pathogenic yeast Candida auris

“…A major determinant of the ability of microbial species to persist in marine environments is the ability to withstand cationic stress due to high salt concentration, and several species of Candida species are frequently found in marine environments [ 37 , 41 , 44 , 45 , 100 , 101 ]. The halotolerance of C .…”

“…A major determinant of the ability of microbial species to persist in marine environments is the ability to withstand cationic stress due to high salt concentration, and several species of Candida species are frequently found in marine environments [37,41,44,45,100,101]. The halotolerance of C. auris (and its hypothesised emergence from the marine environment) could facilitate the survival of C. auris and its subsequent dissemination over large distances [77,80,99].…”

Biocontrol Potential of Sodin 5, Type 1 Ribosome-Inactivating Protein from Salsola soda L. Seeds

“…Finally, we tested two ubiquitous yeasts, Candida parapsilosis and Rhodotorula mucilaginosa , which are often isolated from habitats associated with human and anthropogenic pollution [ 55 , 56 ]. They can be isolated from seawater and salty mud [ 57 , 58 ], but are not primarily associated with salterns or halophytes, which may partly explain their susceptibility to sodin 5. While C. parapsilosis growth was inhibited at higher concentrations (20 and 40 µg/mL) of the protein, R. mucilaginosa growth was inhibited already at the lowest concentration (10 µg/mL).…”

Environmental Sustainability of Solvent Extraction Method in Recycling Marine Plastic Waste

“…The diamond crystal was sterilized with acetone and a background scan was executed. The surface of the sample was analyzed before and after the recycling process [21]. Every sample was pressed against the diamond with a force ranging from 75-80 N to assure that there was an adequate contact between the sample and the ATR crystal [22].…”