Biochemical Factors Contributing to Response Variation among Resistant and Sensitive Clones of Daphnia magna Straus Exposed to Ethyl parathion

Barata, Carlos; Baird, Donald J.; Soares, Amadeu M.V.M.; Guilhermino, Lúcia

doi:10.1006/eesa.2001.2052

Cited by 53 publications

(24 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For the crustacean group, a large number of publications refer to a concentration-dependent inhibition of ChE with OP and carbamate pesticides (Supplemental Data, Table S1). This has been documented for Daphnia magna [30][31][32][33][34], Gammarus pulex [4], Hyalella azteca [35], and Procambarus clarkii [36] with several OPs and carbamates. These observations are in accordance with the expectations based on the mode of action of carbamates and OP pesticides.…”

Section: Cholinesterasementioning

confidence: 95%

“…Several works (Table 3) suggest that a direct relationship between the degree of inhibition and toxicity might not be expected; different pesticides will have different affinities to the active site of AChE and, therefore, different exposure concentrations will be required to achieve 50% of AChE inhibition [29,[31][32][33][34]56,71,72]. Mortality may also result from the indirect effects of pesticides, such as binding to other enzyme systems, e.g., the nonspecific carboxylesterases, making it difficult to correlate AChE depression and mortality.…”

Section: Biomarker Responses and Endpoints Of Higher Biological Organmentioning

confidence: 99%

See 1 more Smart Citation

Cholinesterase and glutathione‐S‐transferase activities in freshwater invertebrates as biomarkers to assess pesticide contamination

Domingues

Agra

Monaghan

et al. 2009

Enviro Toxic and Chemistry

128

View full text Add to dashboard Cite

Abstract-Studies investigating the use of biomarkers in pesticide risk assessment have greatly increased in recent years; however, issues concerning the ecological meaning of enzymatic responses have proved controversial. Ideally a good biomarker response should be modulated by the environmental contaminants alone and demonstrate a predictable behavior towards certain types of toxins. As these premises are rarely observed, the present study aims to outline research that has contributed to an understanding of the behavior of two widely used biomarkers, cholinesterase and glutathione-S-transferase, describing environmental and biotic factors that affect their response in freshwater invertebrates. Studies were performed in the main classes of aquatic invertebrates with these biomarkers and conclusions were reached concerning their behavior towards the main classes of pesticides. Links between biomarker responses and conventional endpoints were evaluated so that ecological relevance could be attributed to enzymatic responses. Toxicity of mixtures was investigated, and cases of synergism and antagonism were pointed out as factors changing the expected toxicity of aquatic systems and leading to misinterpretations of biomarker responses. Finally, the use of biomarkers as a tool for biomonitoring and in situ assays was investigated, with discussion of advantages and disadvantages of their use.

show abstract

Section: Cholinesterasementioning

confidence: 95%

Section: Biomarker Responses and Endpoints Of Higher Biological Organmentioning

confidence: 99%

Cholinesterase and glutathione‐S‐transferase activities in freshwater invertebrates as biomarkers to assess pesticide contamination

Domingues

Agra

Monaghan

et al. 2009

Enviro Toxic and Chemistry

128

View full text Add to dashboard Cite

show abstract

“…They feed on phytoplankton and are preyed upon by aquatic vertebrates and macro-invertebrates. The unique responses of ChE levels in Daphnia when exposed to anticholinesterases have been observed multiple times (Gälli et al, 1994;Guilhermino et al, 1996;Sturm and Hansen, 1999;Barata et al, 2001;Carvalho et al, 2003;Duquesne, 2006;Vesela et al, 2006;Dámasio et al, 2007;Jemec et al, 2007;Printes et al, 2008;Duquesne and Küster, 2010;Coelho et al, 2011;Li and Tan, 2011). According to Sturm and Hansen (1999), up to 80% ChE inhibition was observed in Daphnia after the animals were exposed for 24 h to dichlorvos, parathion, and aldicarb, respectively, at concentration of 0.25, 1, and 250 μg/L.…”

Section: Introductionmentioning

confidence: 99%

Purification and studies on characteristics of cholinesterases from Daphnia magna

Yang

Niu

2013

J. Zhejiang Univ. Sci. B

View full text Add to dashboard Cite

Due to their significant value in both economy and ecology, Daphnia had long been employed to investigate in vivo response of cholinesterase (ChE) in anticholinesterase exposures, whereas the type constitution and property of the enzyme remained unclear. A type of ChE was purified from Daphnia magna using a three-step procedure, i.e., Triton X-100 extraction, ammonium sulfate precipitation, and diethylaminoethyl (DEAE)-Sepharose™-Fast-Flow chromatography. According to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), molecular mass of the purified ChE was estimated to be 84 kDa. Based on substrate studies, the purified enzyme preferred butyrylthiocholine iodide (BTCh) [with maximum velocity (V max )/Michaelis constant (K m )=8.428 L/(min·mg protein)] to acetylthiocholine iodide (ATCh) [with V max /K m =5.346 L/(min·mg protein)] as its substrate. Activity of the purified enzyme was suppressed by high concentrations of either ATCh or BTCh. Inhibitor studies showed that the purified enzyme was more sensitive towards inhibition by tetraisopropylpyrophosphoramide (iso-OMPA) than by 1,5-bis(4-allyldimethylammoniumphenyl) pentan-3-one dibromide (BW284C51). Result of the study suggested that the purified ChE was more like a type of pseudocholinesterase, and it also suggested that Daphnia magna contained multiple types of ChE in their bodies.

show abstract

“…It consumes algae and other small phytoplanktons and in turn acts as prey for freshwater fish and large aquatic invertebrates. Due to its worldwide distribution and the importance of the species in maintaining the sustainability of aquatic ecosystems, the animal has long been noted for its responses in terms of ChE during anticholinesterase exposure (Gälli et al, 1994;Guilhermino et al, 1996;Barata et al, 2001;Carvalho et al, 2003;Duquesne, 2006;Vesela et al, 2006;Damásio et al, 2007;Jemec et al, 2007;Printes et al, 2008;Duquesne and Küster, 2010;Coelho et al, 2011;Li and Tan, 2011).…”

Section: Introductionmentioning

confidence: 99%

Developing antibodies from cholinesterase derived from prokaryotic expression and testing their feasibility for detecting immunogen content in Daphnia magna

Liu

Yuan

2016

J. Zhejiang Univ. Sci. B

View full text Add to dashboard Cite

Abstract:To yield cholinesterase (ChE) from prokaryotic expression, the ChE gene that belongs to Daphnia magna was amplified by reverse transcription-polymerase chain reaction (RT-PCR) using forward primer 5'-CCCYGGNGCSAT GATGTG-3' and reverse primer 5'-GYAAGTTRGCCCAATATCT-3'. To express the gene, one sequence of the amplified DNA, which was able to encode a putative protein containing two conserved carboxylesterase domains, was connected to the prokaryotic expression vector PET-29a(+). The recombinant vector was transformed into Escherichia coil BL21 (DE3). Protein expression was induced by isopropy-D-thiogalactoside. The expressed ChE was used as an immunogen to immunize BALB/c mice. The obtained antibodies were tested for their specificity towards crude enzymes from species such as Alona milleri, Macrobrachium nipponense, Bombyx mori, Chironomus kiiensis, Apis mellifera, Eisenia foetida, Brachydanio rerio, and Xenopus laevis. Results indicated that the antibodies had specificity suitable for detecting ChE in Daphnia magna. A type of indirect and non-competitive enzyme-linked immunosorbent assay (IN-ELISA) was used to test the immunoreactive content of ChE (ChE-IR) in Daphina magna. The detection limit of the IN-ELISA was found to be 14.5 ng/ml at an antiserum dilution of 1:22 000. Results from tests on Daphnia magna exposed to sublethal concentrations of triazophos indicated a maximal induction of 57.2% in terms of ChE-IR on the second day after the animals were exposed to a concentration of 2.10 μg/L triazophos. Testing on animals acclimatized to a temperature of 16 °C indicated that ChE-IR was induced by 16.9% compared with the ChE-IR content detected at 21 °C, and the rate of induction was 25.6% at 10 °C. The IN-ELISA was also used to test the stability of ChE-IR in collected samples. Repeated freezing and thawing had no influence on the outcome of the test. All these results suggest that the polyclonal antibodies developed against the recombinant ChE are as efficient as those developed against the native ChE in detecting ChE content in Daphnia magna.

show abstract

Biochemical Factors Contributing to Response Variation among Resistant and Sensitive Clones of Daphnia magna Straus Exposed to Ethyl parathion

Cited by 53 publications

References 36 publications

Cholinesterase and glutathione‐S‐transferase activities in freshwater invertebrates as biomarkers to assess pesticide contamination

Cholinesterase and glutathione‐S‐transferase activities in freshwater invertebrates as biomarkers to assess pesticide contamination

Purification and studies on characteristics of cholinesterases from Daphnia magna

Developing antibodies from cholinesterase derived from prokaryotic expression and testing their feasibility for detecting immunogen content in Daphnia magna

Contact Info

Product

Resources

About