Biochemical estimation of manganese nanoparticles reactions in native and model biological systems

Abstract

Purpose Due to its physiological importance as a micronutrient, manganese supplementation requires taking into account the possibility of overdosing, which is associated with the initiation of stressogenic effects in plant cells. The use of nanoparticles may reduce the amount of Mn ion administered, especially during foliar treatment. Hence, the aim of the research was to demonstrate the mechanism of action of MnNPs on plant cells and to determine the extent of the stress-inducing reaction of these NPs. Methods A response to manganese nanoparticles was studied in seedlings of two wheat cultivars, model system of plant cell membranes and compared with cell lines (U-937, HL-60, HUT-78, COLO-720L). The nanoparticles (MnNPs as Mn3O4) were at the same concentrations (125 and 250 mg/mL) by foliar application to plants and to the cell culture media. Results The administration of NPs enhanced the content of Mn in plant cells, indicating its penetration through the leaf surface and initiated the oxidative stress measures as an increase in enzyme activity, starch accumulation, and a decrease in chlorophyll synthesis. Moreover, a rise in the electrokinetic potential of the chloroplast membrane surface and the reconstruction of their hydrophobic parts toward an increase in fatty acid saturation was found. Conclusion The study demonstrated that the stress response in plant cells was initiated up to 250 mg/mL of MnNPs. The same concentration resulted in reduction of cell viability, especially in U-937 line.

Manganese Nanoparticles: Synthesis, Mechanisms of Influence on Plant Resistance to Stress, and Prospects for Application in Agricultural Chemistry

“…Treatment with Mn NPs resulted in disruption of photosynthesis, resulting in increased starch concentration and reduced chlorophyll synthesis. Moreover, a rise in the electrokinetic potential of the chloroplast membrane surface and the reconstruction of their hydrophobic parts toward an increase in fatty acid saturation was found by Sieprawska et al…”

“…Treatment with Mn NPs resulted in disruption of photosynthesis, resulting in increased starch concentration and reduced chlorophyll synthesis. Moreover, a rise in the electrokinetic potential of the chloroplast membrane surface and the reconstruction of their hydrophobic parts toward an increase in fatty acid saturation was found by Sieprawska et al 171 FeO x NPs, MnO x NPs, and bimetallic MnO x /FeO x NPs had positive effects on plant Zea mays growth, in particular seed germination rate, root growth, and corn seedling biomass growth, and thus can be used as micronutrient nanofertilizers. 172 Nanopriming of rice seeds with MnS NPs improved germination percentage, seed germination index, average germination time, dry weight, and viability index, and reduced the number of seeds and seedlings affected by late blight.…”

“…167 A response to Mn NPs was studied in seedlings of two spring wheat cultivars, model system of plant cell membranes. 171 The Mn 3 O 4 NPs were used at the same concentrations 125 and 250 mg/mL by foliar application to plants and to the cell culture media. The administration of NPs enhanced the content of Mn in plant cells, indicating its penetration through the leaf surface and initiated the oxidative stress responses as an increase in enzyme activity, starch accumulation, and a decrease in chlorophyll synthesis.…”

“…A response to Mn NPs was studied in seedlings of two spring wheat cultivars, model system of plant cell membranes . The Mn 3 O 4 NPs were used at the same concentrations 125 and 250 mg/mL by foliar application to plants and to the cell culture media.…”