Biochemical Effects of Mebendazole on Trichinella spiralis Larvae

Nollin, Sonja De; Bossche, H. : Van den

doi:10.2307/3278626

Cited by 40 publications

(8 citation statements)

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“…In previous studies, other researchers determined the glycogen content in Trichinella using the less specific anthrone method. They established that the glycogen concentration in the viable larvae is about 0.0784 μg/larva (De Nollin and Van den Bossche, 1973). This value corresponds with the data obtained in the current study.…”

Section: Invasive Capacity Of Trichinella Muscle Larvaesupporting

confidence: 92%

“…The muscle larvae of Trichinella spiralis are extremely rich in glycogen, which constitutes 16% of their dry weight (Beckett and Boothroyd, 1962;Castro and Fairbairn, 1969). Many researchers used the methods of histochemical staining and electron microscopy to find the main locations of glycogen deposition at different stages of Trichinella biological cycle (De Nollin and Van den Bossche, 1973;Ferguson and Castro, 1973, Rudneva et al, 2019Sidor and Andreyanov, 2019). It was established that the total amount of glycogen increases during the maturation of the larva in muscle tissue, and then sharply decreases when larvae grow, molt and reach puberty in the intestine of the new host.…”

Section: Introductionmentioning

confidence: 99%

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The Role of Glycogen in Biological Cycle of Trichinella spiralis

Sidor¹,

Andreyanov²

2020

jwpr

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The energy sources of Trichinella spiralis change in both the muscular and intestinal stages of its life in the host organism. The purpose of this study was to investigate the quantitative changes in glycogen concentration during the life cycle of T. spiralis in a host organism. Trichinella spiralis was passaged on laboratory rodents under the vivarium conditions. Sixty-nine white rats (350 g each) were infected with T. spiralis at a dose of 5 muscle larvae/gram of body weight. The animals were euthanized at different time periods from the start of the experiment. Trichinella muscle larvae were isolated by artificial fermenting meat mince in gastric juice. To determine the viability of Trichinella larvae, they were heated to 38 ± 2 ° C for 10 minutes their motor activity was investigated. (38 ± 2 °C). To determine the invasive properties of T. spiralis at different stages of its development in rats, the muscular larvae isolated from the rat muscles were used to infect laboratory mice. The invasive capacity of T. spiralis was assessed on day 45 post-infection. For the study of intestinal Trichinella larvae, laboratory rats were not fed a day before infection. Adult nematodes were isolated from the small intestine of laboratory rats at 3, 6 and 24 hours post-infection. The nematodes were counted in the Migacheva-Kotelnikov chamber in each individual sample. The concentration of glycogen in the nematodes was calculated according to the quantitative method for determining glycogen in Trichinella larvae. Low glycogen levels in the muscle larvae were observed on day 14 post-infection. The glycogen concentration in muscular larva was 0.0054 ± 0.0027 μg/ larva on day 21, 0.0136 ± 0.0024 μg/ larva on day 28, and 0.0771 ± 0.0025 μg/ larva on day 45 after the rats were infected. Maximum concentration of glycogen was recorded 4 months post-infection (0.0930 ± 0.0029 μg/larva). Further, the glycogen level began to decrease slowly. In the 20th month post-infection, after infection, the amount of glycogen in a Trichinella larva was 0.0786 ± 0.0023 μg. In the body of intestinal nematodes, 3 hours after infecting the animals, the glycogen concentration was reduced to 0.0472 ± 0.0003 μg in one nematode. The same time period later, it reached to value of 0.0272 ± 0.0002 μg. In intestinal T. spiralis, which remained in the small intestine of rats for 24 hours, the glycogen was not detected. The amount of glycogen at the muscle stage of T. spiralis development was extremely important in the first hours of the helminth's residing in the host's intestines. Energy requirements during the period when the helminth cannot obtain enough food depend on the glycogen content. When the glycogen concentration in the parasite is insufficient, the Trichinella larvae will lose their invasion capacity.

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Section: Invasive Capacity Of Trichinella Muscle Larvaesupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

The Role of Glycogen in Biological Cycle of Trichinella spiralis

Sidor¹,

Andreyanov²

2020

jwpr

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“…Differential binding affinities between nematode and mammalian tubulin may explain the selective toxicity of benzimidazoles, as pointed out by several workers (18)(19)(20). Alternatively, other sites of action, such as the inhibition of fumarate reductase activity (14)(15)(16), or the restriction of glucose uptake followed by depletion of glycogen reserves (14,15,17) …”

Section: Discussionmentioning

confidence: 99%

Relationships Between the Anthelmintic Activity of Eight Derivatives of Benzimidazole Carbamates Against Trichinella Spiralis and Their Chemical Structures

Latif

Surin

1993

JJMSB

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“…Finally, two additional effects have been cited in relation to benzimidazoles: (1) the inhibition of uptake of glucose and depletion of glycogen reserves and (2) the inhibition of secretion of acetylcholinesterase. The first effect was initially described by Van den Bossche (1972), and then later observed in several helminths (De Nollin & Van den Bossche, 1973;McCracken & Taylor, 1983), although Simpkin & Coles (1976) and others could not confirm this fact. In a recent paper, Van den Bossche, Rochette & Horig (1982) proposed the hypothesis that impairment of glucose uptake would be induced by the disappearance of cytoplasmic microtubules in the tegumental or intestinal cells of cestodes and nematodes.…”

Section: Introductionmentioning

confidence: 96%

The mode of action of some benzimidazole drugs onTrichinella spiralis

1987

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In an attempt to find possible targets for benzimidazole action in muscle-stage larvae of Trichinella spiralis, the effects of mebendazole and thiabendazole were tested in vivo by oral treatment of infested mice and in vitro by including these anthelmintics in an adequate maintenance medium containing decapsulated larvae. The effects of the anthelmintics on succinate dehydrogenase and fumarate reductase activities, measured in the mitochondrial fraction obtained from the in vivo- or in vitro-treated larvae showed that only thiabendazole causes significant inhibition of fumarate reductase activity. On the other hand, measurements of free glucose, glycogen reserves and soluble protein in the treated larvae indicate that in vivo, mebendazole and thiabendazole clearly diminish free glucose levels, although in vitro only mebendazole produces the same diminution. Both the glycogen and protein contents of the larvae remained unchanged after treatment in vivo or in vitro. The importance of these findings with regard to a possible site of action for mebendazole and thiabendazole is discussed.

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Biochemical Effects of Mebendazole on Trichinella spiralis Larvae

Cited by 40 publications

References 7 publications

The Role of Glycogen in Biological Cycle of Trichinella spiralis

The Role of Glycogen in Biological Cycle of Trichinella spiralis

Relationships Between the Anthelmintic Activity of Eight Derivatives of Benzimidazole Carbamates Against Trichinella Spiralis and Their Chemical Structures

The mode of action of some benzimidazole drugs onTrichinella spiralis

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