Biochemical Criteria for the Evaluation of Drug Efficiency on Adjuvant Arthritis and Nephrotoxic Serum Nephritis in the Rat: Studies with Phenylbutazone, <scp>L</scp>-Asparaginase, Colchicine, Lysine Acetylsalicylate, and Pyridinol Carbamate

Sternberg, M.; Peyroux, J.; Grochulski, A; Engler, R; Feret, J; Moisy, M; Lagrue, G; Jayle, M F

doi:10.1139/y75-053

Cited by 10 publications

(4 citation statements)

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“…The podocyte-toxic effects of doxorubicin leading to podocyte loss and effacement and at later stages to focal segmental or global glomerulosclerosis are well established (6,39). A direct tubulotoxic effect, however, is not typical, at least in rats (42). Hence, the development of tubulointerstitial inflammation and fibrosis seen in this model more likely reflects the tubulotoxic effects of proteinuria and albuminuria.…”

Section: Discussionmentioning

confidence: 79%

Serum- and glucocorticoid-inducible kinase 1 in doxorubicin-induced nephrotic syndrome

Artunc

Nasir²,

Amann³

et al. 2008

American Journal of Physiology-Renal Physiology

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Doxorubicin-induced nephropathy leads to epithelial sodium channel (ENaC)-dependent volume retention and renal fibrosis. The aldosterone-sensitive serum- and glucocorticoid-inducible kinase SGK1 has been shown to participate in the stimulation of ENaC and to mediate renal fibrosis following mineralocorticoid and salt excess. The present study was performed to elucidate the role of SGK1 in the volume retention and fibrosis during nephrotic syndrome. To this end, doxorubicin (15 μg/g body wt) was injected intravenously into gene-targeted mice lacking SGK1 ( sgk1 −/−) and their wild-type littermates ( sgk1 +/+). Doxorubicin treatment resulted in heavy proteinuria (>100 mg protein/mg crea) in 15/44 of sgk1 +/+ and 15/44 of sgk1 −/− mice leading to severe nephrotic syndrome with ascites, lipidemia, and hypoalbuminemia in both genotypes. Plasma aldosterone levels increased in nephrotic mice of both genotypes and was followed by increased SGK1 protein expression in sgk1 +/+ mice. Urinary sodium excretion reached signficantly lower values in sgk1 +/+ mice (15 ± 5 μmol/mg crea) than in sgk1 −/− mice (35 ± 5 μmol/mg crea) and was associated with a significantly higher body weight gain in sgk1 +/+ compared with sgk1 −/− mice (+6.6 ± 0.7 vs. +4.1 ± 0.8 g). During the course of nephrotic syndrome, serum urea concentrations increased significantly faster in sgk1 −/− mice than in sgk1 +/+ mice leading to uremia and a reduced median survival in sgk1 −/− mice (29 vs. 40 days in sgk1 +/+ mice). In conclusion, gene-targeted mice lacking SGK1 showed blunted volume retention, yet were not protected against renal fibrosis during experimental nephrotic syndrome.

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Section: Discussionmentioning

confidence: 79%

Serum- and glucocorticoid-inducible kinase 1 in doxorubicin-induced nephrotic syndrome

Artunc

Nasir²,

Amann³

et al. 2008

American Journal of Physiology-Renal Physiology

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“…The hemoglobin count was determined by acid haematin method [13]. On day 21, blood was withdrawn by retro-orbital puncture and serum was used for estimation of biochemical markers like SGOT, SGPT, ALP, RA factor, Creatinine and serum uric acid [14,15]. Radiographs were taken for the hind paw of all groups and examined for soft tissue swelling, bony erosions and narrowing of spaces between the joints [16][17][18].…”

Section: Haematological Biochemical Radiological Studiesmentioning

confidence: 99%

Synthesis and Evaluation of Phthalate Analogue of Diclofenac Against Freund’s Complete Adjuvant Induced Arthritis in Rat

Manimekalai

Shukla

Sudhakar

et al. 2019

Int J Pharm Pharm Sci

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Objective:The objective of the present study is to evaluate the effect of Phthalate analogues of diclofenac in Freund's complete adjuvant (FCA) induced Arthritis in the rat.Methods: Twenty four female albino wistar rats were enrolled in this study and are divided into 4 groups (six each). The groups were designed as follows: Group I: vehicle control, Group II: arthritic control, Group III: diclofenac treated, Group IV: phthalate analogue of diclofenac treated. Various assessments such as anti-arthritic activity, biochemical estimations, haematological parameters, ulcerogenesis, radiological and histopathological studies were evaluated.Results: Arthritic control group exhibited significant increase in the level of paw volume, arthritic score (p<0.0001), Serum glutamic pyruvic transaminase (SGPT) (p<0.001), Serum glutamic oxaloacetic transaminase (SGOT) p<0.01), rheumatoid arthritis factor, C-reactive protein (CRP), White Blood Cells (WBC), Creatinine and uric acid and a significant decrease in Red Blood Cells (RBC). Increased swelling of joints, bony destruction and profound ulceration were observed in the Arthritic control group. All these conditions were reversed in diclofenac and phthalate analogue of diclofenac groups. Conclusion:We conclude that phthalate analogue of diclofenac shows potent anti-arthritic activity with milder ulceration when compared to diclofenac treatment. Acute oral toxicity studies of phthalate analogue of diclofenac-OECD 423 guidelineAcute oral toxicity studies were performed using a phthalate analogue of diclofenac at the dose level of 300 mg/kg orally in mice. AnimalsHealthy adult female Albino Wister Rats (150-200 g: 24 rats) were obtained from Swamy Vivekananda College of Pharmacy, Tiruchengodu.

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“…SOD enzyme activity was calculated in plasma according to the method of Misra and Fridovich (1972) with slight modifications. SOD levels were determined at 460 nm and results expressed as U/mg protein …”

Section: Methodsmentioning

confidence: 99%

“…SOD levels were determined at 460 nm and results expressed as U/mg protein. 60 Measurement of inflammatory cytokines (TNF-α, IL-6, INFγ, IL-10): Blood was withdrawn from experimental rats. The serum was revived and chilled at −20 °C until evaluation.…”

Section: Methodsmentioning

confidence: 99%

Naringin in Combination with Isothiocyanates as Liposomal Formulations Potentiates the Anti-inflammatory Activity in Different Acute and Chronic Animal Models of Rheumatoid Arthritis

et al. 2020

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Combination of drugs is extensively used to treat chronic inflammatory disease. Naringin (NAR), sulforaphane (SFN), and phenethyl isothiocyanate (PEITC) are nutraceuticals with promising anti-inflammatory properties. However, their clinical effectiveness gets hindered because of low aqueous solubility and poor bioavailability. In the current study, two combinations of liposome (NAR + SFN and NAR + PEITC) were prepared and studied thoroughly in different in vivo models of acute and chronic models of inflammation. The encapsulation efficiency of NAR, SFN, and PEITC in the combination liposomal formulations (CLFs) prepared with 1,2-dipalmitoyl-sn-glycero-3-phosphocholine/cholesterol/1,2-distearoyl-sn-glycero-3-phosphoethanolamine -020CN (15:4:1 M ratio) was determined to be 79.8 ± 4.2, 46.5 ± 3.6, and 78.5 ± 3.2%, respectively. The CLFs were characterized by differential scanning calorimetry, Xray diffraction, dynamic light scattering, and Fourier transform infrared spectroscopy. The physicochemical results showed that the preparations were monodisperse (PDI 0.062−0.248) in water with an average size from 140.5 to 165.6 nm and a zeta potential of −47.3 to −53.3 mV. Dissolution studies in vitro showed a slower release of PEITC (>90%, 6 h) in comparison to that of SFN (3 h). Here, we are the first to report the antiarthritic activity of CLF of NAR + SFN and NAR + PEITC in the Freund's complete adjuvant (FCA)-induced arthritic model. At an intraperitoneal dose (375 + 375 μg/mL) for 3 weeks, the NAR + PEITC liposome significantly improves both % paw edema and arthritic score compared to their free drug combinations in FCA rats. Most importantly, hematological and biochemical results showed improved anemic conditions with significant changes in the SGOT, SGPT, and ALP levels. The ELISA results showed similar trends of increased cytokine (IL-10) and decreased inflammation markers (TNF-α, IL-6, IFN-γ). Histological evaluations showing reduction in cell infiltration, pannus formation, and bone and cartilage destruction further confirm and validate the antiarthritic activity of the CLF. This comprehensive study reveals the effectiveness of combination liposomes of poorly soluble antiinflammatory molecules (NAR, SFN, PEITC) in the treatment of arthritis.

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Biochemical Criteria for the Evaluation of Drug Efficiency on Adjuvant Arthritis and Nephrotoxic Serum Nephritis in the Rat: Studies with Phenylbutazone, L-Asparaginase, Colchicine, Lysine Acetylsalicylate, and Pyridinol Carbamate

Cited by 10 publications

References 1 publication

Serum- and glucocorticoid-inducible kinase 1 in doxorubicin-induced nephrotic syndrome

Serum- and glucocorticoid-inducible kinase 1 in doxorubicin-induced nephrotic syndrome

Synthesis and Evaluation of Phthalate Analogue of Diclofenac Against Freund’s Complete Adjuvant Induced Arthritis in Rat

Naringin in Combination with Isothiocyanates as Liposomal Formulations Potentiates the Anti-inflammatory Activity in Different Acute and Chronic Animal Models of Rheumatoid Arthritis

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