Biochemical compositions and transcriptome analysis reveal dynamic changes of embryonic development and nutrition metabolism in Chinese sturgeon (Acipenser sinensis)

Zhou, Meng; Zhang, Dezhi; Long, Xianmei; Chen, Wangwang; Jiang, Wei; Chen, Pei; Tan, Qingsong

doi:10.1016/j.aquaculture.2023.740003

Cited by 2 publications

(1 citation statement)

References 54 publications

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“…After determining the lipid content, the dried residue was methylated with boron trifluoride (BF 3 ) in methanol, and then the methyl esterified samples were analyzed by gas chromatography (Agilent 6890, Santa Clara, CA, USA) with an OmegawaxTM320 column (Agilent 6890, Santa Clara, CA, USA). Chromatography was carried out as described in our recent literature (Zhou et al 2023 ). Individual fatty acid methyl ester (FAME) peaks were identified according to their retention times in comparison to the retention times of known FAMEs in a FAME standard (Nu-Chek Prep.…”

Section: Methodsmentioning

confidence: 99%

Photoheterotroph improved the growth and nutrient levels of Chlorella vulgaris and the related molecular mechanism

Long,

Zhang,

Yang

et al. 2024

Appl Microbiol Biotechnol

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Microalgae are rich in fatty acids, proteins, and other nutrients, which have gained the general attention of researchers all over the world. For the development of Chlorella vulgaris in food and feed industry, this study was conducted to investigate the differences in C. vulgaris’ growth and nutritional components under different culture conditions (autotrophic, heterotrophic, photoheterotrophic) and the internal factors through cell counting in combination with transcriptome and nutrient analyses. The results showed that, under the photoheterotrophic condition, Chlorella’s growth and the contents of lipid and protein were significantly higher than that under the heterotrophic condition, and the moisture content was lower than that under the heterotrophic condition. The saturated fatty acid content under the photoheterotrophic condition was the lowest, while the polyunsaturated fatty acid content was significantly higher than those under the other two conditions. There were 46,583 differentially expressed genes (DEGs), including 33,039 up-regulated DEGs (70.93%) and 13,544 down-regulated DEGs (29.07%), under the photoheterotrophic condition in comparison with the autotrophic condition. The fold change between the two conditions of samples of up-regulated genes was higher than that of the down-regulated genes. The KEGG enrichment showed that the up-regulated DEGs in the photoheterotrophic condition were significantly enriched in 5 pathways, including protein processing in endoplasmic reticulum pathway, photosynthesis pathway, photosynthesis-antenna protein pathway, endocytosis pathway, and phosphonate and phosphinate metabolism pathway. DEGs related to fatty acid metabolic pathways were significantly enriched in the fatty acid biosynthesis pathway and the biosynthesis of unsaturated fatty acid pathway. The qPCR analysis showed that the expression pattern of the selected genes was consistent with that of transcriptome analysis. The results of this study lay a theoretical foundation for the large-scale production of Chlorella and its application in food, feed, and biodiesel. Key points • Nutrient levels under photoheterotrophic condition were higher than other conditions. • Six important pathways were discovered that affect changes in nutritional composition. • Explored genes encode important enzymes in the differential metabolic pathways.

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Section: Methodsmentioning

confidence: 99%

Photoheterotroph improved the growth and nutrient levels of Chlorella vulgaris and the related molecular mechanism

Long,

Zhang,

Yang

et al. 2024

Appl Microbiol Biotechnol

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Metabolomics in sturgeon research: a mini-review

Liu,

Naganuma

2024

Fish Physiol Biochem

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Sturgeons are ancient fish, with 27 species distributed in the Northern Hemisphere. This review first touches upon the significance of sturgeons in the context of their biological, ecological, and economic importance, highlighting their status as “living fossils” and the challenges they face in genomic research due to their diverse chromosome numbers. This review then discusses how omics technologies (genomics, transcriptomics, proteomics, and metabolomics) have been used in sturgeon research, which so far has only been done on Acipenser species. It focuses on metabolomics as a way to better understand how sturgeons work and how they react to their environment. Specific studies in sturgeon metabolomics are cited, showing how metabolomics has been used to investigate various aspects of sturgeon biology, such as growth, reproduction, stress responses, and nutrition. These studies demonstrate the potential of metabolomics in improving sturgeon aquaculture practices and conservation efforts. Overall, the review suggests that metabolomics, as a relatively new scientific tool, has the potential to enhance our understanding of sturgeon biology and aid in their conservation and sustainable aquaculture, contributing to global food security efforts.

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Biochemical compositions and transcriptome analysis reveal dynamic changes of embryonic development and nutrition metabolism in Chinese sturgeon (Acipenser sinensis)

Cited by 2 publications

References 54 publications

Photoheterotroph improved the growth and nutrient levels of Chlorella vulgaris and the related molecular mechanism

Photoheterotroph improved the growth and nutrient levels of Chlorella vulgaris and the related molecular mechanism

Metabolomics in sturgeon research: a mini-review

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