Biochemical composition of the spermatozoal plasma membrane in normal and heat-stressed boars

Abstract

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Immunofluorescence Reveals Ubiquitination of Retained Distal Cytoplasmic Droplets on Ejaculated Porcine Spermatozoa

“…The effect of a retained DD on fertility is less well defined, although there is some evidence suggesting a negative impact for stored boar semen used in artificial insemination programs (Larsson et al, 1984; Zeuner, 1992; Waberski et al, 1994; Althouse, 1998). An elevated incidence of retained DDs has been described in conjunction with biochemically altered plasma membranes in heat‐stressed boars (Althouse, 1992). Additionally, boar sperm with retained CDs have a reduced binding affinity for porcine oviductal epithelial explants in culture (Petrunkina et al, 2001).…”

Participation of phosphofructokinase, malate dehydrogenase and isocitrate dehydrogenase in capacitation and acrosome reaction of boar spermatozoa

“…Capacitation is followed by an exocytotic event termed acrosome reaction that is an absolute requisite for fertilization (Yanagimachi, ). In boar spermatozoa, capacitation is dependent upon calcium and bicarbonate, but does not appear to require albumin, possibly due to the remarkably low cholesterol/phospholipid ratio of boar spermatozoon (Althouse, ; Tardif, Dubé, & Bailey, ). Bicarbonate has been identified as the essential capacitating agent for several animal species, including the pig (Harrison & Gadella, ).…”

Current Concepts of Molecular Events During Bovine and Porcine Spermatozoa Capacitation

“…Incubation of murine spermatozoa in media devoid of bicarbonate, calcium, or albumin prevents capacitation as assessed by the ability of these spermatozoa to undergo a zona pellucidainduced acrosome reaction and fertilize oocytes in vitro [Visconti et al 1995]. Similarly, porcine spermatozoa capacitation is dependent upon calcium and bicarbonate, but does not appear to require albumin, possibly due to the remarkably low cholesterol: phospholipid ratio of porcine spermatozoa [Althouse 1992;Tardif et al 2003]. However, porcine spermatozoa capacitation can be inhibited by an increase in membrane cholesterol mediated by cyclodextrin (Galantino-Homer et al 2006).…”