Biochemical composition as a function of fruit maturity stage of bell pepper (Capsicum annum) inoculated with Bacillus amyloliquefaciens

Cisternas‐Jamet, Jonathan; Salvatierra‐Martínez, Ricardo; Vega‐Gálvez, Antonio; Stoll, Alexandra; Uribe, Elsa; Goñi, María Gabriela

doi:10.1016/j.scienta.2019.109107

Cited by 36 publications

(30 citation statements)

References 42 publications

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“…From a redox viewpoint, it has been found that reactive oxygen species (ROS) metabolism is also affected during fruit ripening, leading to major changes in total soluble reducing equivalents and the antioxidant capacity in fruits [41]. The profile of the major non-enzymatic antioxidants, including ascorbate, glutathione, carotenoids and polyphenols, has been followed during ripening in pepper fruits [4,11,12,[42][43][44][45][46], but less is known on how enzymatic antioxidants evolve with this physiological process. These enzyme systems basically include superoxide dismutase (SOD), catalase (CAT) and the ascorbate-glutathione cycle as the primary defense barriers against ROS, and some NADP-dehydrogenases as a secondary system to help the antioxidative enzymatic block.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Profile of Pepper (Capsicum annuum L.) Fruits Containing Diverse Levels of Capsaicinoids

et al. 2020

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Capsicum is the genus where a number of species and varieties have pungent features due to the exclusive content of capsaicinoids such as capsaicin and dihydrocapsaicin. In this work, the main enzymatic and non-enzymatic systems in pepper fruits from four varieties with different pungent capacity have been investigated at two ripening stages. Thus, a sweet pepper variety (Melchor) from California-type fruits and three autochthonous Spanish varieties which have different pungency levels were used, including Piquillo, Padrón and Alegría riojana. The capsaicinoids contents were determined in the pericarp and placenta from fruits, showing that these phenyl-propanoids were mainly localized in placenta. The activity profiles of catalase, total and isoenzymatic superoxide dismutase (SOD), the enzymes of the ascorbate–glutathione cycle (AGC) and four NADP-dehydrogenases indicate that some interaction with capsaicinoid metabolism seems to occur. Among the results obtained on enzymatic antioxidants, the role of Fe-SOD and the glutathione reductase from the AGC is highlighted. Additionally, it was found that ascorbate and glutathione contents were higher in those pepper fruits which displayed the greater contents of capsaicinoids. Taken together, all these data indicate that antioxidants may contribute to preserve capsaicinoids metabolism to maintain their functionality in a framework where NADPH is perhaps playing an essential role.

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Section: Introductionmentioning

confidence: 99%

Antioxidant Profile of Pepper (Capsicum annuum L.) Fruits Containing Diverse Levels of Capsaicinoids

et al. 2020

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“…Conversely, during ripening, NO content and catalase activity lower [43]. The content of the main non-enzymatic antioxidants (ascorbate, glutathione, carotenoids and polyphenols) has also been investigated in pepper fruit ripening [7,8,28,[44][45][46][47][48][49]. NO treatment was shown to prevent some of the changes undergone by these parameters, thus confirming the delaying effect of the ripening process indicated above [38,50,51].…”

Section: Introductionmentioning

confidence: 60%

Identification of Compounds with Potential Therapeutic Uses from Sweet Pepper (<em>Capsicum annuum</em> L.) Fruits and Their Modulation by Nitric Oxide (NO)

Guevara¹,

Domínguez-Anaya²,

Ortigosa³

et al. 2021

Preprint

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Plant species are precursors of a wide variety of secondary metabolites that, besides having useful activity for themselves, can also be used by humans for their consumption and economic benefit. Pepper (Capsicum annuum L.) fruit is not only a common food and spice source, but it also stands out for containing high amounts of antioxidants (such as vitamins C and A), polyphenols and capsaicinoids. Particular attention has been paid to capsaicin, whose anti-inflammatory, antiproliferative and analgesic activities, have been proven. Due to the potential interest in pepper metabolites for human use, in this project, we carried out an investigation to identify new bioactive compounds of this crop is carried out. To achieve this, we developed a metabolomic approach, using an HPLC (high-performance liquid chromatography) separative technique coupled to metabolite identification by high resolution mass spectrometry (HRMS). After chromatographic analysis and data processing against metabolic databases, 12 differential bioactive compounds were identified in sweet pepper fruits, including quercetin and its derivatives, L-tryptophan, phytosphingosin, FAD, gingerglycolipid A, tetrahydropentoxylin, blumenol C glucoside, colnelenic acid and capsoside A. The abundance of these metabolites varied depending on the ripening stage of the fruits, either immature green or ripe red. We also studied the variation of these 12 metabolites upon treatment with exogenous nitric oxide (NO), a free radical gas involved in a good number of physiological processes in higher plants such as germination, growth, flowering, senescence, and fruit ripening, among others. Overall, it was found that the content of the analysed metabolites was modulated by the ripening stage and by the presence of NO. The metabolic pattern followed by quercetin and its derivatives, as a consequence of the ripening stage and NO treatment, was also corroborated by transcriptomic analysis of genes involved in the synthesis of these compounds. This opens new research windows on the pepper fruit’s bioactive compounds with nutraceutical potentiality, where biotechnological strategies can be applied for optimizing the level of these beneficial compounds.

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“…The benefits of PGPB application include enhancing root and shoot growth, nutrient uptake, and hydration; increasing chlorophyll content; and strengthening resistance to disease [ 28 ]. The beneficial effects of PGPB have been demonstrated for many crops: wheat [ 29 ], tobacco [ 30 ], mustard [ 31 ], tomatoes and potato [ 32 ], bell peppers [ 33 ], cucumbers [ 34 ], and perennial crops such as Miscanthus sp. [ 5 , 35 ] and switchgrass [ 36 ].…”

Section: Introductionmentioning

confidence: 99%

Bioprospecting of a Novel Plant Growth-Promoting Bacterium Bacillus altitudinis KP-14 for Enhancing Miscanthus × giganteus Growth in Metals Contaminated Soil

Pranaw

Pidlisnyuk

Trögl

et al. 2020

Biology

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Use of plant growth-promoting bacteria (PGPB) for cultivation of the biofuel crop Miscanthus × giganteus (Mxg) in post-military and post-mining sites is a promising approach for the bioremediation of soils contaminated by metals. In the present study, PGPB were isolated from contaminated soil and screened for tolerance against abiotic stresses caused by salinity, pH, temperature, and lead (Pb). Selected strains were further assessed and screened for plant growth-promoting attributes. The isolate showing the most potential, Bacillus altitudinis KP-14, was tested for enhancement of Mxg growth in contaminated soil under greenhouse conditions. It was found to be highly tolerant to diverse abiotic stresses, exhibiting tolerance to salinity (0–15%), pH (4–8), temperature (4–50 °C), and Pb (up to 1200 ppm). The association of B. altitudinis KP-14 with Mxg resulted in a significant (p ≤ 0.001) impact on biomass enhancement: the total shoot and dry root weights were significantly enhanced by 77.7% and 55.5%, respectively. The significant enhancement of Mxg biomass parameters by application of B. altitudinis KP-14 strongly supports the use of this strain as a biofertilizer for the improvement of plant growth in metal-contaminated soils.

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Biochemical composition as a function of fruit maturity stage of bell pepper (Capsicum annum) inoculated with Bacillus amyloliquefaciens

Cited by 36 publications

References 42 publications

Antioxidant Profile of Pepper (Capsicum annuum L.) Fruits Containing Diverse Levels of Capsaicinoids

Antioxidant Profile of Pepper (Capsicum annuum L.) Fruits Containing Diverse Levels of Capsaicinoids

Identification of Compounds with Potential Therapeutic Uses from Sweet Pepper (<em>Capsicum annuum</em> L.) Fruits and Their Modulation by Nitric Oxide (NO)

Bioprospecting of a Novel Plant Growth-Promoting Bacterium Bacillus altitudinis KP-14 for Enhancing Miscanthus × giganteus Growth in Metals Contaminated Soil

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