Biochemical composition and antioxidant capacity of extracts from Podophyllum hexandrum rhizome

Abstract

BackgroundPodophyllum hexandrum Royle (P. hexandrum) is a perennial herb and widely used in clinic. The present study was designed to separate and identify the biochemical composition and antioxidant capacity of extracts from P. hexandrum rhizome.MethodsThe ethyl acetate and ethanol extracts from P. hexandrum rhizome were analyzed by GC-MS (gas chromatography–mass spectrometry), and the antioxidant capacity of the extracts and the components was tested by using the DPPH (2, 2-diphenylpicrylhydrazyl) and FRAP (Ferric reducing/antioxidant power) assays.ResultsThe rhizome extracts had greater antioxidant capacity than the petiole extracts in DPPH and FRAP assays. About 16 kinds of main reactive oxygen components were identified in the extracts. Components of PADE (Phthalic acid, diisobutyl ester), BADE (1,2-Benzenedicarboxylic acid, diisooctyl ester), Polyneuridine, PODD (Podophyllotoxin, deoxy), β-Sitosterol and POD (Podophyllotoxin) showed the antioxidant capacity in some degree. PODD, POD, and Polyneuridine showed stronger antioxidant capacity with the IC50 and FRAP values of 9.61 ± 0.81 and 2923.98 ± 21.89 μM, 9.98 ± 0.24 and 2847.27 ± 14.82 μM, and 13.37 ± 0.35 and 2404.32 ± 36.88 μM, respectively, than the positive control ASA (Ascorbic acid) with the values of 60.78 ± 1.22 and 1267.5 ± 30.24 μM (P < 0.01).ConclusionsPODD, POD, and Polyneuridine are very critical for the antioxidant capacity in the extract of P. hexandrum rhizome. These results provide useful biochemical basis and information for the potential use of this plant.

Changes in antioxidant capacity, levels of soluble sugar, total polyphenol, organosulfur compound and constituents in garlic clove during storage

“…Trace DSQ GC-MS from American Finnigan Company was used. The GC and MS conditions were described by the literature (Li et al, 2012) with slightly modification, and the specific steps as follows. 1N NOWAX quartz capillary column: 30 m × 0.32 cm × 0.25 mm; column temperature: 50-190 • C; procedure temperature: 5 • C/min; carrier gas: He; vaporizer temperature: 280 • C; the MS conditions were: Ion source: EI; temperature: 200 • C; ionizing voltage: 70 eV; electric current of collection: 300 A; electric current of emission: 1 mA; resolution: 500; mass: 10-500.…”

“…The measurement was described by the literature (Benzie and Strain, 1996;Li et al, 2012), and the specific steps as follows. The 300 L FRAP reagent [prepared ex tempore by mixing 10 volumes of 300 mmol/L acetate buffer, pH 3.6, with 10 mmol/L TPTZ in 40 mmol/L HCl, and 20 mmol/L FeCl 3 ·6H 2 O at 10:1:1 (v/v/v)] and the 10 l standard samples (FeSO 4 ·7H 2 O, 500 M) or test samples of H 2 O, ethanol and ethyl acetate in section 2.2 (10 mg/mL 80% methanol) were mixed.…”

“…The measurement was performed according to the literature (Li et al, 2012(Li et al, , 2013, and the specific steps as follows. The extracts of H 2 O, ethanol and ethyl acetate in Section 2.2 were diluted with 80% methanol with concentration of 10 mg/mL, and then 50 l of sample solution was added into 950 l of 10 −4 M DPPH methanol solution.…”

Antioxidant Capacity Connection with Phenolic and Flavonoid Content in Chinese Medicinal Herbs

“…The free radical scavenging activity of DPPH was measured according to the description [14]. This is one of the few stable and commercially available organic nitrogen radical assays [15].…”

GC-MS analysis and antimicrobial effects of methanol stem bark extract of Trilepisium madagascriense DC.

“…Indole which has been isolated from the stem bark ethanol extract of Aspidosperma ramiflorum (Tanaka et al, 2006), stem bark methanol extract of Aspidosperma olivaceum (Chierrito et al, 2014) and stem bark ethanol extract of Winchia calophylla (Zhu et al, 2005) has been reported to have antibacterial effects against S. aureus, E. coli and P. aeruginosa (Medeiros et al, 2011). Ethyl isoallocholate which have been isolated from the ethanol extract of Podophyllum hexandrum rhizome (Li et al, 2012), ethanol extract of Kirganelia reticulate aerial parts (Muthulakshmi et al, 2012) and ethanol extract of Feronia elephantum stem bark (Muthulakshmi et al, 2012Sudha et al, 2013 have been reported to possess antimicrobial activities (Singariya et al, 2012). Phenol has been isolated from the methanolic extract of Ficus religiosa stem bark (Manorenjitha et al, 2013), and methanol extract of Psidium guajava root bark (Velmurugan et al, 2012).…”