Biochemical Characterization of Some Species of Trypanosoma (Schizotrypanum) from Bats (Microchiroptera) *

We performed a phylogenetic character mapping on 26 stocks of Trypanosoma cruzi, the parasite responsible for Chagas disease, and 2 stocks of the sister taxon T. cruzi marinkellei to test for possible associations between T. cruzi-subspecific phylogenetic diversity and levels of protein expression, as examined by proteomic analysis and mass spectrometry. We observed a high level of correlation (P < 10) between genetic distance, as established by multilocus enzyme electrophoresis, and proteomic dissimilarities estimated by proteomic Euclidian distances. Several proteins were found to be specifically associated to T. cruzi phylogenetic subdivisions (discrete typing units). This study explores the previously uncharacterized links between infraspecific phylogenetic diversity and gene expression in a human pathogen. It opens the way to searching for new vaccine and drug targets and for identification of specific biomarkers at the subspecific level of pathogens.infectious disease | parallel evolution | gene expression | Chagas disease | parasite

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“…The pathogenicity of T. cruzi marinkellei has been explored at length (34). It is morphologically undistinguishable from T. cruzi.…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic character mapping of proteomic diversity shows high correlation with subspecific phylogenetic diversity in Trypanosoma cruzi

Tellería

Biron²,

Brizard

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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“…30 Moreover, molecular and biological studies of such trypanosomes suggested that those differing from T. cruzi sensus stricto should be treated as distinct subspecies. 31,32 Here, three stocks isolated from bats and one from a sylvatic R. prolixus belonged to branch 2 and exhibited high genetic distances to T. cruzi and T. rangeli species. Therefore they should belong to other species.…”

Section: Differentiation Between American Trypanasoma Stocksmentioning

confidence: 91%

Isoenzyme clustering of Trypanosomatidae Colombian populations.

Montilla¹,

GUHL²,

Jaramillo³

et al. 2002

The American Journal of Tropical Medicine and Hygiene

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Abstract. Thirty-six Trypanosomatidae stocks isolated from various hosts and geographical areas in Colombia and 7 others from Bolivia, Chile, Honduras and Panama have been surveyed by Multilocus Enzyme Electrophoresis (MLEE). Part of the Colombian stocks were previously characterized by morphology and biological behavior as belonging to Trypanosoma cruzi and Trypanosoma rangeli taxa, others were unknown species. The genetic variability observed at 13 different loci was considerable, since 38 zymodemes could be distinguished and 2 upper branches were observed. The first branch corresponded to T. cruzi and was divided in the two major phylogenetic subdivision of T. cruzi (T. cruzi I, T. cruzi II). The majority of the Colombian T. cruzi stocks (92%) felt into T. cruzi I. Only two stocks, isolated from sylvatic mammals, belonged to T. cruzi II. Among T. cruzi I, we did not observed any additional phylogenetic subdivision and host-dependent genotype specificity. The second branch was genetically very heterogeneous and included all T. rangeli stocks, the stocks isolated from bats and one stock isolated from a sylvatic R. prolixus vector. The stocks belonging to T. rangeli presented only one locus instead of two for the malic enzyme system. Since, the upper level of resolution of the isoenzyme method was exceeded, the current clustering study failed to draw a clear distinction between such a diverse set of Trypanosomatidae species.

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“…The tribe Cavernicolini Usinger, 1944, is composed of only two species of the genus Cavernicola Barber, 1937, namely Cavernicola lenti Barrett &Arias, 1985 andCavernicola pilosa Barber, 1937. Both members of the Cavernicolini tribe (as the Cavernicola genus) were initially described from C. pilosa.…”

Section: Introductionmentioning

confidence: 99%

First cytogenetic study of Cavernicola pilosa Barber, 1937 (Hemiptera, Triatominae)

Souza¹,

Alevi²,

Ribeiro³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Cavernicola pilosa is a triatomine species that lives in caves and feeds on bat blood. This vector has a wide geographical distribution, and is found in Brazil, Colombia, Panama, Peru, and Venezuela. Little is known about the reproductive biology of this species, because most previous studies have only characterized its morphology, morphometry, ecology, and epidemiology. Therefore, this study aimed to obtain preliminary data related to spermatogenesis in C. pilosa by conducting cytogenetic analysis. Analysis of the heterochromatic pattern of C. pilosa during the initial prophases revealed that heterochromatic blocks are only present in the sex chromosomes. Based on the analyses of the meiotic metaphase and prophases, we found that the sex determination system of C. pilosa is XY and the chromosomes are holocentric. C. pilosa spermatids are filamentous and have long flagella. It was not possible to detect corpuscle 13890 E.S. Souza et al.©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 13889-13893 (2015) or filament heteropycnosis in spermatids of this species. The initial cytogenetic data presented in this study are important in characterizing the spermatogenesis and heterochromatic patterns of C. pilosa. Our results suggest that adaptation to troglodytism did not result in differences in spermatogenesis in this vector.

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Biochemical Characterization of Some Species of Trypanosoma (Schizotrypanum) from Bats (Microchiroptera) *

Cited by 79 publications

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Phylogenetic character mapping of proteomic diversity shows high correlation with subspecific phylogenetic diversity in Trypanosoma cruzi

Phylogenetic character mapping of proteomic diversity shows high correlation with subspecific phylogenetic diversity in Trypanosoma cruzi

Isoenzyme clustering of Trypanosomatidae Colombian populations.

First cytogenetic study of Cavernicola pilosa Barber, 1937 (Hemiptera, Triatominae)

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