Biochemical characterization of sericin isolated from cocoons of tropical tasar silkworm Antheraea mylitta raised on three different host plants for its prospective utilization

Jena, Karmabeer; Kumari, Ruchi; Pandey, Jay Prakash; Kar, Prasanta Kumar; Akhtar, Javed; Singh, Ashutosh; Gupta, V.P.; Sinha, Ajit

doi:10.1016/j.aspen.2021.07.018

Cited by 4 publications

(2 citation statements)

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“…The thickness of cocoon, which is one of the quantitative traits of cocoon phenotype (Deka and Kumari, 2013;Nayak and Guru, 1998), is directly related to food plants (Jena et al, 2021) of its substrate is temperature dependent. Cocoonase specifically acts upon sericin (Sakata et al, 2022) to loosen the silk fiber at suitable pH and temperature (Unajak et al, 2015).…”

Section: Resultsmentioning

confidence: 99%

Antheraea mylitta cocoonase: An explorable protease

Rani¹,

Pandey²,

Jena³

et al. 2023

JEB

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Aim: The study was conducted to explore the insect secreted protease Antheraea mylitta cocoonase. Methodology: The cocoons of eleven ecoraces were collected followed by observation of cocoonase secretion behaviour, cocoonase collection, purification, and its quantification. In addition, the thermal stability of cocoonase and gel diffusion assay were investigated. Results: Antheraea mylitta cocoons of eleven ecoraces were collected from different eco-pockets of India. Subsequently, the cocoonase secretion behaviour of different ecoraces during emergence was studied. It was observed that the quantity and concentration of cocoonase secreted depends upon various factors such as cocoon size, cocoon stiffness, and the habitat in which silkworm is reared. To observe the proteolytic specificity of A. mylitta cocoonase, a gel diffusion assay was conducted using casein and gelatin as probable substrates. The presence of hydrolytic ring confirmed protease activity. In addition, the thermal stability of cocoonase was also observed by protease activity. Interpretation: The present study concludes that Antheraea mylitta cocoonase is an explorable protease that needs further exploration to unveil the unspecified properties of this insect-secreted protease, which can find beneficial applications in silk and other industries. Key words: Antheraea mylitta, Cocoonase, Ecoraces, Gel diffusion assay, Temperature stability

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Section: Resultsmentioning

confidence: 99%

Antheraea mylitta cocoonase: An explorable protease

Rani¹,

Pandey²,

Jena³

et al. 2023

JEB

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“…Always have two plastic tubs with water and a 2% bleaching powder to 0.5% slaked lime solution (add 20 grammes of bleaching powder and 5 grammes of slaked lime to one litre of water) close to the bushes so that you can wash your hands before and after handling the worms. (Jena et al 2020) [27] . According to Singh et al 2020 [68] , dusting slaked lime and bleaching powder (9:1) on the ground under bushes and over the bushes once in each instar and three times in the fifth instar has been found to be useful in preventing the spread of bacterial diseases.…”

Section: Review Of Literaturementioning

confidence: 98%

Know how on essential chemicals used in Tasar sericulture

Nanda,

Nadaf,

et al. 2024

Int. J. Adv. Biochem. Res.

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Tasar larvae are exposed to the environment, attracting a variety of diseases, pests and predators, resulting in increased larval mortality and a reduction in tasar silk production in India. The most common diseases are virosis, pebrine, muscardine and bacteriosis. Chemicals especially disinfectants play a significant role in tasar sericulture. Chemicals help to control and prevent the spread of diseases, pests and other harmful organisms that can negatively impact tasar silkworm and its host plants. While it is possible to practice tasar sericulture without the use of chemicals, it may be more challenging and less efficient and could result in lower yields and lower quality produce. The use of recommended disinfectants in sericulture ensures clean and hygienic crop production. By implementing proper sanitation and pest management practices, farmers can help to ensure the health and safety of their crops and promote sustainable sericulture practices. Hence for the intended study, the various chemicals and disinfectants used were deliberated in detail. Their importance and usage etc studied were presented .However, it is important to use these chemicals safely and responsibly to minimize any potential negative impacts on the environment and avoid harming the environment wildlife and human health. Hence, understanding and knowing what and how the chemicals used in sericulture and their benefits is important for protecting the environment, human health and the economic viability of tasar sericulture.

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