Biochemical Characterization of Protease Isolated from Different Parts of Choerospondias axillaris (Lapsi)

Abstract

Proteases were isolated from leaf, bark and root of Choerospondias axillaris, locally called Lapsi. Choerospondias axillaris is a dioecous, deciduous fruit bearing plant with multiple daily uses. The protease was extracted with 0.1 M phosphate buffer of pH 7 and then precipitated successively with TCA and ammonium sulfate. The protease from leaf showed maximum activity at pH 9, temperature at 20°C. While, bark protease showed optimum pH 5 and temperature 60°C. In case of root the optimum pH was 10 and the optimum temperature 35°C. The optimum time of incubation leaf, bark and root was 15 minute. The bell shaped curve was obtained for the effect of enzyme conc. with optimum enzyme conc. 50 µg for leaf, 30 µg for bark and 50 µg for root. The K m and V max value of leaf were 5.61 µM and 185.18 pmol/ min, K m and V max value of bark were 2.36 µM and 82.64 pmol/min. While for root, the K m and V max values were 1.53 µM and 52.91 pmol/min.

Economic Analysis of Lapsi (Choerospondias axillaris Roxb.) Production in Parbat District, Nepal

“…Li et al, (2016) reported the presence of medicinally important phytochemicals in both the pulp and peel of Lapsi. Besides, Protease: a proteolytic enzyme can be isolated from leaf, bark, root (Upadhyay, Magar and Thapa, 2013) and fruit pulp (Prajapati, Sharma and Agrawal, 2009) of the Lapsi. Thus, it is a potential product for national as well as international market (Paudel et al, 2003).…”