Biochemical Characterization of &lt;i&gt;Ralstonia solanacerum&lt;/i&gt; Causing Bacterial Wilt of Brinjal in Bangladesh

Article HistoryRalstonia solanacearum (E.F. Smith) Yabuuchi, is the cause of bacterial wilt of tomato and one of the most severe pathogens of solanaceous crops with a very wide host range. There is little information on R. solanacearum strains in Maseno region in Kenya despite the fact that bacterial wilt of tomato is of economic importance. The objective of this study was to isolate the strains of R. solanacearum of tomato and characterize morphologically and biochemically Ralstonia solanacearum races and biovars from infected tomato plants in Maseno region. This study was conducted at Maseno University in the Department of Botany at Microbiology Laboratory. Ten diseased tomato plants from Maseno, Mariwa, Seme and Hollo were collected and cut into small sections (0.5-1cm). The plant sections were then plated onto Triphenyl tetrazolium chloride (TZC) media. Profiling of the pathogen was done morphologicaly using culture techniques; biochemical tests included Gram staining test, Potassium hydroxide test, Catalase oxidase test, Gas production test, Starch hydrolysis test and sugar utilization test. The races were then identified by pathogenicity test on wide host range. All the isolates had fluidal pinkish red centered colonies on TZC media, they were Gram negative, potassium hydroxide solubility positive, produced gas from glucose, ooze test positive and did not hydrolyse starch which is typical of R. solanacerum. R. solanacearum strains from infected tomato plants in Maseno region were race 3 biovar 1 and race 3 biovar 3. All isolates were pathogenic on tomato plants.

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“…Present results agreed with those reported by [24][25][26] and Maji and Chakrabartty [27]. This is the characteristic feature of R.…”

Section: Morphological Profiling Of Ralstonia Solanacearumsupporting

confidence: 83%

“…shows the difference between avirulent colonies that look dark red from the fluidal virulent that are white with pink center [25,29]. In the present study, the colonies were fluidal whitish with a pink center, indicating virulent species of R. solanacearum.…”

Section: Morphological Profiling Of Ralstonia Solanacearummentioning

confidence: 54%

Isolation and Characterisation of Ralstonia Solanacearum Strains of Tomato Wilt Disease from Maseno, Kenya

Khasabulli

Musyimi

Miruka

et al. 2017

Journal of Asian Scientific Research

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“…On this medium, typical bacterial colonies appear fluidal, irregular in shape, and white with pink centres after 2 to 5 days incubation at 28ºC as reported by Champoiseau (2008). R. solanacearum produced fluidal colonies with pink or light red colour on TZC media after 24 hours of inoculation as reported previously by Rahman et al (2010).…”

Section: Identification Of Virulent/avirulent Strains Of R Solanacearummentioning

confidence: 63%

“…5, No. 6;2013 affected by this pathogen, phenotype and genotype of Ralstonia solanacearum, its wide geographical distribution, and the range of environmental conditions conducive to bacterial wilt (Rahman et al, 2010). …”

Section: Incidence and Severity Of Bacterial Wiltmentioning

confidence: 99%

“…Gram staining reaction, Potassium hydroxide solubility test, Kovac's oxidase test, Levan test and Sugar fermentation test were performed for confirmation of R. solanacearum isolates as described previously by Rahman et al (2010) and Hossain (2006). Single isolate of R. solanacearum from each group was randomly selected for biochemical tests.…”

Section: Biochemical Tests For the Identification Of R Solanacearummentioning

confidence: 99%

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Determination of Races and Biovars of Ralstonia solanacearum Causing Bacterial Wilt Disease of Potato

Ahmed¹,

Islam²,

Meah³

et al. 2013

JAS

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A survey was carried out in some selected potato growing districts of Bangladesh during December to February 2011 to know the status of bacterial wilt of potato caused by Ralstonia solanacearum in terms of its incidence and severity. The results showed that the highest wilt incidence was recorded in Munshigonj (22.65%), followed by Nilphamari (19.98%) and the lowest incidence was recorded in Jamalpur (9.07%). The highest bacterial wilt severity was recorded in Munshigonj (3.80), while the lowest wilt severity was recorded in Jamalpur (2.90). A total of 44 isolates (R. solanacearum) were obtained from the wilted potato plant samples i.e. 20 from Munshigonj, 17 from Nilphamari and 7 from Jamalpur and the isolates were divided into three groups. The production of pink or light red colour with whitish margin on TZC medium by the bacterial isolates indicated all groups of R. solanacearum isolates were virulent. The results of pathogenicity test revealed that all groups of R. solanacearum isolates were able to cause wilt symptoms in potato plants and brown rot symptoms in potato tuber. On the other hand, all biochemical tests were used for the identification of R. solanacearum isolates. The biovar test using the oxidization of disaccharides (sucrose, lactose, maltose) and sugar alcohols (manitol, sorbitol and dulcitol) by R. solanacearum isolates confirmed that all groups of R. solanacearum isolates belong to biovar III. The race identification of R. solanacearum isolates by pathogenicity test on brinjal, tomato, tobacco and chilli indicating a narrow host range (only in potato) and were categorized in race 3. Therefore, the R. solanacearum isolates causing bacterial wilt of potato in Bangladesh were belonging to Biovar III and Race 3.

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Rep-PCR Analyses Reveal Genetic Variation of Ralstonia solanacearum Causing Wilt of Solanaceaous Vegetables in Bangladesh

et al. 2022

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Biochemical Characterization of <i>Ralstonia solanacerum</i> Causing Bacterial Wilt of Brinjal in Bangladesh

Cited by 21 publications

References 13 publications

Isolation and Characterisation of Ralstonia Solanacearum Strains of Tomato Wilt Disease from Maseno, Kenya

Isolation and Characterisation of Ralstonia Solanacearum Strains of Tomato Wilt Disease from Maseno, Kenya

Determination of Races and Biovars of Ralstonia solanacearum Causing Bacterial Wilt Disease of Potato

Rep-PCR Analyses Reveal Genetic Variation of Ralstonia solanacearum Causing Wilt of Solanaceaous Vegetables in Bangladesh

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