Biochemical characterization of different types of adherence of Vibrio species to fish epithelial cells

Wang, X. H.; Leung, Ka Yin

doi:10.1099/00221287-146-4-989

Cited by 43 publications

(35 citation statements)

References 59 publications

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“…The information carried in the ECM is decoded by lectins (Arason 1996). Such carbohydrate-lectin interactions have been described for pathogens (Venkataraman et al 1997, Ewart et al 1999, Wang & Leung 2000, Zamze et al 2002. For symbiotic bacteria, similar mechanisms have been proposed.…”

Section: Introductionmentioning

confidence: 84%

Attachment mechanism in a highly specific association between ectosymbiotic bacteria and marine nematodes

Nussbaumer¹,

Bright²,

Baranyi³

et al. 2004

Aquat. Microb. Ecol.

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The marine Stilbonematinae (Nematoda) are known for their highly specific mutualistic association with thiotrophic ectosymbiotic bacteria. The mechanism mediating recognition and binding between symbionts and host was studied in 5 host species. When incubated with D-mannose and L-rhamnose the symbionts detached in 2 species, Laxus cosmopolitus and L. oneistus, most likely due to competitive interactions with sugars involved in the binding mechanism; 3 other species, Stilbonema maium, Eubostrichus topiarius and E. dianae, did not lose their bacteria during any tested sugar incubations. Incubations with lectins binding specifically to D-glucose/D-mannose (ConA, concanavalin agglutinin) and to D-mannose (NPA, Narcissus pseudonarcissus agglutinin) respectively, both in vivo and on ultrathin sections, confirmed that accessible D-mannose is located on the symbionts of L. cosmopolitus, but not on the host's surface. Our results showed an involvement of D-mannose and L-rhamnose residues of the bacterial surface in the attachment mechanism. We hypothesize that the recognition and binding of the environmentally transmitted symbionts in the 2 Laxus species, which harbor only 1 phylotype of symbiotic γ-proteobacterium each, is most probably mediated through a yet unknown mannose/rhamnose-specific lectin of host origin.

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Section: Introductionmentioning

confidence: 84%

Attachment mechanism in a highly specific association between ectosymbiotic bacteria and marine nematodes

Nussbaumer¹,

Bright²,

Baranyi³

et al. 2004

Aquat. Microb. Ecol.

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“…infections (Toranzo & Barja 1993). In addition, the ECP fractions from pathogenic Vibrio appear to play a major role during disease and the enzymatic components of this fraction have been implicated as important factors necessary for invasion, survival and proliferation in host fish (Balebona et al 1998, Wang et al 1998, Wang & Leung 2000. Although ECP is an important factor in pathogenesis, a positive correlation between the pathogenicity of a particular strain of Vibrio and the virulence of the ECP from the same strain cannot be assumed as cytotoxicity of ECP prepared from nonpathogenic Vibrio strains can also occur (Toranzo et al 1983).…”

Section: Discussionmentioning

confidence: 99%

Modulated heat shock protein expression during pathogenic Vibrio alginolyticus stress of sea bream

Deane

Li²,

Woo³

2004

Dis. Aquat. Org.

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The effects of infective Vibrio alginolyticus and its extracellular product (ECP) on host fish function are not well understood. In this study a partial biochemical characterization of the ECP from an infective strain of V. alginolyticus isolated from diseased silver sea bream Sparus sarba was achieved and the effects of live V. alginolyticus and ECP on hepatic heat shock protein (hsp) expression was compared. The ECP fraction was found to contain several hydrolytic enzymes including both haemolytic and proteolytic activities. Intramuscular administration of ECP to sea bream resulted in vibriosis with similar pathological signs as those observed with live V. alginolyticus administration. Using quantitative immunoassays we assessed the levels of the major hsp families, hsp90, hsp70 and hsp60, in hepatic tissue of diseased sea bream between 12 and 48 h post-infection. Throughout the infective period, live V. alginolyticus did not alter hsp90 whereas ECP significantly reduced hepatic hsp90 during the late stages of acute infection. The levels of hsp70 were found to be rapidly and drastically increased with both live V. alginolyticus and ECP. The transcript levels of both gene members of the hsp70 family (hsc70 and hsp70) were significantly increased with both live V. alginolyticus and ECP. The levels of hsp60 remained unchanged with both live V. alginolyticus and ECP. The data presented in this study is the first report describing an effect of both live V. alginolytus and ECP on hsp expression in diseased fish.KEY WORDS: Fish · Disease · Vibrio · Heat shock protein · hsp90 · hsp70 · hsp60 Resale or republication not permitted without written consent of the publisherDis Aquat Org 62: [205][206][207][208][209][210][211][212][213][214][215] 2004 When normal cellular processes are adversely affected the synthesis of a group of proteins belonging to the heat shock protein (hsp) families (hsp90, hsp70 and hsp60) are rapidly increased. Members of these different hsp families play different roles in the cell; for example, the hsp90 family is involved in steroid receptor formation and protein folding (Smith & Toft 1993, Pratt 1997, the hsp70 family is necessary for protein synthesis, translocation and protein folding (Gething & Sambrook 1992), and mitochon-drial bound hsp60 is defined as being involved in protein stability and folding (Cheng et al. 1989, Ostermann et al. 1989, Martin et al. 1992. Indeed all of these groups of hsps have been demonstrated to be upregulated in fish during abiotic stress (Iwama et al. 1998). Evidence from mammalian studies has suggested that hsps are important during disease as they may play critical roles in immune function and protection (Jacquier-Sarlin et al. 1994), antigen presentation (DeNagel & Pierce 1993) and non-specific immune responses (Guzik et al. 1999). Although strong evidence exists for an important role of hsps in disease responses for mammals, much less is known about pathogenic stress and its effect on hsp90, 70 and 60 expression in fish. A better understanding...

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“…T5 on infection results are striking (Table 1). Vibrio cells undergoing starvation survival (including stationary phase cells in batch cultures) increase their adhesive properties, and at the same time produce starvation survival proteins (Kjelleberg et al 1983, Wang & Leung 2000. In-depth molecular studies are needed to link PUWS and the properties of stationary phase cells of pathogenic Vibrio species to their virulence (Paul et al 1991, Ben-Haim et al 2003, McDougald et al 2003, Nielsen et al 2006, Sussman et al 2008).…”

Section: Properties Of the Inoculummentioning

confidence: 99%

Vibrio sp. causing Porites ulcerative white spot disease

Arboleda¹,

Reichardt²

2010

Dis. Aquat. Org.

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The causative agent of the Indo-Pacific coral disease, Porites ulcerative white spot syndrome (PUWS), that affects Porites spp. and a few other coral genera has so far remained unidentified. Inoculation of thiosulphate citrate bile sucrose (TCBS) agar with tissue material from Porites cylindrica infected with white spot produced colonies of approximately 3 mm diameter consisting of Gram-negative, motile, non-sucrose-fermenting, slightly curved rods with a minimum NaCl requirement of 0.3%. Three of these putative Vibrio sp. isolates were used for infection trials that included different stages of cell growth. Four modes of inoculation and 3 stages of bacterial cell growth were considered for testing Koch's postulates. Stationary phase cells proved more consistently infectious than did exponentially growing or starved cells using a 1-step immersion technique at cell concentrations of 10 4 cells ml -1. A 1-step immersion technique proved more reliable in producing signs of white spot than did other techniques, such as injection, smearing and 2-step immersion of the inoculum. At inoculum densities >10 4 cells ml -1 further signs of disease, such as tissue degradation and bleaching, also became evident. At elevated temperatures (> 29°C) bleaching remained absent for at least 2 mo from non-inoculated corals serving as controls, but was observed in artificially infected coral fragments. Of the 9 seawater aquaria containing healthy specimens of P. cylindrica, 6 showed signs of white spot 15 d after infection with an isolate tentatively identified as Vibrio sp. Based on 99% similarity of its 16S rRNA gene sequence and selected phenotypical features, this isolate revealed a close relationship to V. natriegens and V. parahaemolyticus. KEY WORDS: Indopacific coral disease · Porites · Causative agent · Vibrio Resale or republication not permitted without written consent of the publisherDis Aquat Org 90: [93][94][95][96][97][98][99][100][101][102][103][104] 2010 ora spp., Montastrea spp. and Pocillopora damicornis (Rosenberg et al. 2007). Vibrio species are widespread copiotropic constituents of mesophilic microbial communities that favor organic pollution from shellfish and fish cultivation in coastal marine environments (Colwell et al. 1977, Colwell 1996, Reichardt et al. 2007). This group also harbors the causative agents of an extremely wide range of diseases of commercial marine fishes and shellfish (Bruno et al. 1998, Robertson et al. 1998, Lacoste et al. 2001). At year-round surface water temperatures between 27 and 33°C coral reefs in the vicinity of intensive milkfish Chanos chanos mariculture at Cape Bolinao (Lingayen Gulf, Philippines) are particularly exposed to high densities of different populations of Vibrio spp. (W. T. Reichardt et al. unpubl. data). Thus, a highly selective enrichment medium for marine vibrios, such as thiosulphate citrate bile sucrose (TCBS) agar (Bolinches et al.1988), was considered as first choice for isolating test strains from white spots of diseased Porites cylindrica to ...

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Biochemical characterization of different types of adherence of Vibrio species to fish epithelial cells

Cited by 43 publications

References 59 publications

Attachment mechanism in a highly specific association between ectosymbiotic bacteria and marine nematodes

Attachment mechanism in a highly specific association between ectosymbiotic bacteria and marine nematodes

Modulated heat shock protein expression during pathogenic Vibrio alginolyticus stress of sea bream

Vibrio sp. causing Porites ulcerative white spot disease

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