Biochemical Characterization of a Novel Thermostable Ulvan Lyase from Tamlana fucoidanivorans CW2-9
Yan Xu,
Jin Li,
Lu An
et al.
Abstract:Ulvan is a complex sulfated polysaccharide extracted
from Ulva, and ulvan lyases can degrade ulvan through
a β-elimination
mechanism to obtain oligosaccharides. In this study, a new ulvan lyase,
EPL15085, which belongs to the polysaccharide lyase (PL) 28 family
from Tamlana fucoidanivorans CW2-9,
was characterized in detail. The optimal pH and salinity are 9.0 and
0.4 M NaCl, respectively. The K
m and V
max of recombinant EPL15085 toward ulvan are
0.80 mg·mL–1 and 11.22 μmol·min –1 mg–1·mL–1, respectively. Une… Show more
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