Biochemical characterization and identification of bacterial strains isolated from drinking water sources of Kohat, Pakistan

Abstract

SPE IPMAs the pure drinking water is inevitable for good health, it is necessary to evaluate it for bacterial contamination. This study was conducted on the different drinking water sources of Kohat, a Northern-Western District of Pakistan. Sampling was done from different drinking water sources including tap water, tube well water, home-well, bore-well and springs. Physiochemical analyses including pH, temperature, turbidity, total dissolved solids and electrical conductivity showed that all water samples were within ranges of the values prescribed by the World Health Organization. A total of 79 bacteria isolated from different samples were characterized. Eighty two percent of the strains were Gram negative and 64% of the total Gram positive bacteria were spore forming. The physiological characterization showed that 30.4% of the total bacterial strains were obligate aerobes while the rest were facultative anaerobes. Biochemical characterization and identification depicted enormous bacterial diversity where sixteen genera could be tentatively identified. Identification of 24 of the strains was further validated by using API 20E kit. Furthermore, the selected strains were analyzed for pH, temperature optimization and NaCl tolerance. Pseudomonas sp were the most abundant bacteria followed by Bacillus sp. Some of the coliform bacteria could also be identified which present a potential health hazard

Molecular Docking of Multidrug Resistant Klebsiella pneumoniae from River Water of Klang Valley, Malaysia

“…Physicochemical parameters of water samples including physical properties, pH, temperature ( 0 C), DO (mg/L) and BOD (mg/L) were determined on site 14 .…”

“…Resistant bacteria then adapt to environmental conditions and become vectors for transmission of antibiotic resistance 12 . Also, construction or industries effluents into aquatic ecosystem can affect the physical, chemical and biological properties of water bodies resulting in poor water quality that facilitates the evolution and transmission of resistance to environmental bacteria 14 . These pollutions are especially common in Klang River which covers the most densely populated areas in Malaysia including many hospitals, household areas, industries and constructions sites.…”

One-Point Cellulose Adsorbents for Water Purification

“…A number of biochemical tests were performed for the isolation, characterization and identification of bacterial isolates using standard methods [28][29][30].…”

Investigation of Sewage and Drinking Water in Major Healthcare Centres for Bacterial and Viral Pathogens

“…Sterilized media were poured into Petri plates, followed by spreading of concentrated water samples with the help of a sterilized loop. Petri plates were incubated at 35°C for 24 h (Also 48 hours to get the correct density) and 5 weeks for MTB, followed by sub-culturing of colonies on fresh selective media at 34°C and 36°C [14]. This step of colony sub-culturing was repeated 3 times for confirmation of the resultant colonies.…”

“…Although numerous research has been conducted to address the detection and origination of pathogens in both drinking water and wastewater [12,13], however, insufficient studies have done explicitly about tracing the occurrence of pathogens in water sources near healthcare facilities [14,15].…”