Biochemical Characterization and Biologic Actions of Two Toxins (D-l and D-2) from Clostridium difficile

Banno, Yoshiko; Kobayashi, T.; Kono, H; Watanabe, Kunitomo; Ueno, Kazue; Nozawa, Yoshinori

doi:10.1093/clinids/6.supplement_1.s11

Cited by 157 publications

(104 citation statements)

References 28 publications

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“…It is possible that a third toxin of C.dz$icile, reported by Banno et al (1984) and unrelated to toxins A and B, could be responsible for watery fluid secretion, but there are two reasons why we think that this is not probable. First, the test system used by Banno et al (1984) was the rabbit ileum, which is almost certainly a less relevant model for PMC disease than rabbit colon. Second, in only one of several attempts was the presence of such a toxin demonstrated in our laboratory (T. J.…”

Section: T J Mitchell J M Ketley D W Burdon D C a Candy Amentioning

confidence: 99%

Biological mode of action of Clostridium difficile toxin A: a novel enterotoxin

Mitchell

Ketley

Burdon

et al. 1987

Journal of Medical Microbiology

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Section: T J Mitchell J M Ketley D W Burdon D C a Candy Amentioning

confidence: 99%

Biological mode of action of Clostridium difficile toxin A: a novel enterotoxin

Mitchell

Ketley

Burdon

et al. 1987

Journal of Medical Microbiology

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“…This bacterial species produces two antigenically and biologically distinct toxins implicated in the pathogenesis of these diseases (Banno et al, 1984;Bartlett et al, 1978;Lyerly et al, 1986). Toxin A has enterotoxic and weakly cytotoxic activities and causes fluid accumulation and mucosal damage in several animal models (Banno et al, 1984;Mitchell et al, 1986). Toxin B has potent cytotoxic activity and alters the morphology of a variety of cultured cells (Banno et al, 1984;Meador & Tweten, 1988;Sullivan et ul., 1982).…”

Section: Introductionmentioning

confidence: 99%

Prevention of Clostridium difficile-induced experimental pseudomembranous colitis by Saccharomyces boulardii: a scanning electron microscopic and microbiological study

Castex

Corthier²,

Jouvert

et al. 1990

Journal of General Microbiology

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The ability of Succhuromyces boulardii to protect mice against intestinal pathology caused by toxinogenic Clostridiurn dzBcile was studied. Different regions of the intestine of experimental mice were prepared for observation by scanning electron microscopy or homogenized for C. dzBcile enumeration and quantification of toxin A by enzyme immunoassay and toxin B by cytotoxicity. The test group was treated for 6 d with an S.boulurdii suspension in drinking water and challenged with C. dz&le on day 4. The three control groups were: axenic mice, mice treated with only S. boulurdii and mice only challenged with C. dz@cile. The results showed that: (i) 70% of the mice infected by C. dzjicile survived when treated with S. boulurdii; (ii) the C. dzBcile-induced lesions on the small and large intestinal mucosa were absent or markedly less severe in S. boulurdii-treated mice; and (iii) there was no decrease in the number of C. dzBcile but rather a reduction in the amount of toxins A and B in S. boulardii-treated mice.

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“…Second, Banno et al (1984) reported a labile toxin that produced a watery fluid response in the rabbit ileal loop test. However, in our laboratory, only one of numerous attempts was successful in repeating the findings of Banno and co-workers.…”

Section: Introductionmentioning

confidence: 99%