Biochemical Characteristics of Penicillium crustosum FP 11 Xylanase II and an Assessment of the Properties of Xylanases Produced by the Genus Penicillium

Bittencourt, Jorge William Fischdick; Arfelli, Vanessa Cristina; Lunkes, Jaina Caroline; Torre, Carla Lieko Della; Silva, Jose L. C.; Maller, Alexandre; Simão, Rita de Cássia Garcia; Kadowaki, Marina Kimiko

doi:10.9734/arrb/2020/v35i730248

Cited by 3 publications

(3 citation statements)

References 33 publications

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“…The xylanase from Penicillium genus was preferred over Trichoderma and Aspergillus genera because it could work in acidic and high-temperature conditions [ 2 ]. Thus, many Penicillium species were published for their xylanase activity, such as P. crustosum [ 18 ], P. janczewskii [ 19 ] , P. ramulosum [ 20 ] , P. rolfsii [ 21 ] , P. funiculosum [ 22 ], P. sclerotiorum [ 23 ], P. citrinum [ 24 , 25 ] and P. chrysogenum [ 26 ]. To our knowledge, the xylanase-producing ability of P. menonorum has not been explored since it was reported for taxonomy in 2015 [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

“…P. menonorum SP10 produced two xylanase isoforms with 45 kDa and 54 kDa. The multiple xylanases were also found in other Penicillium species, such as P. oxalicum GZ-2 [ 28 ], P. ramulosum N1 [ 20 ], P. janczewskii [ 19 ] and P. crustosum FP 11 [ 18 ]. This may be due to Penicillium species containing many xylanase genes.…”

Section: Discussionmentioning

confidence: 99%

“…The multiple xylanase isoforms help these fungi to deal with the hemicellulose complex and degrade it more efficiently [ 28 ]. The molecular weight of P. menonorum SP10’s xylanase differed from most Penicillium species which were reported under 35 kDa [ 18 ]. Only some produced xylanase of more than 40 kDa, such as P. verruculosum (65 kDa) [ 21 ], P. funiculosum (46 kDa) [ 30 ] and P. oxalicum GZ-2 (43 kDa) [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

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Isolation and Characterization of Xylanase from a Novel Strain, Penicillium menonorum SP10

Luong,

Poeaim,

Tangthirasunun

2023

Mycobiology

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Xylanase has been applied in various sectors, such as biomass conversion, paper, pulp, textiles, and pharmaceutical industries. This study aimed to isolate and screen potential xylanase-producing fungi from the soil of Suphan Buri Province, Thailand. Fifteen fungi were isolated, and their xylanase activities were tested by the qualitative method. The result showed that isolate SP3, SP10 and SP15 gave high xylanase activity with potency index (PI) of 2.32, 2.01 and 1.82, respectively. These fungi were selected for the xylanase quantitative test, isolate SP10 performed the highest xylanase activity with 0.535 U/mL. Through molecular methods using the β-tubulin gene, isolate SP10 was identified as Penicillium menonorum . The xylanase characteristics from P. menonorum SP10 were determined, including the xylanase isoforms and the optimum pH and temperature. The xylanase isoforms on SDS-PAGE indicated that P. menonorum SP10 produced two xylanases (45 and 54 kDa). Moreover, its xylanase worked optimally at pH 6 and 55 °C while reaching 61% activity at 65 °C. These results proposed P. menonorum SP10 as a good candidate for industrial uses, especially in poultry feed and pulp industries, to improve yield and economic efficiency under slightly acidic and high-temperature conditions.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Isolation and Characterization of Xylanase from a Novel Strain, Penicillium menonorum SP10

Luong,

Poeaim,

Tangthirasunun

2023

Mycobiology

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Construction of an expression platform for fungal secondary metabolite biosynthesis in Penicillium crustosum

Zhou,

Chen,

2024

Appl Microbiol Biotechnol

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Filamentous fungi are prolific producers of bioactive natural products and play a vital role in drug discovery. Yet, their potential cannot be fully exploited since many biosynthetic genes are silent or cryptic under laboratory culture conditions. Several strategies have been applied to activate these genes, with heterologous expression as one of the most promising approaches. However, successful expression and identification of new products are often hindered by host-dependent factors, such as low gene targeting efficiencies, a high metabolite background, or a lack of selection markers. To overcome these challenges, we have constructed a Penicillium crustosum expression host in a pyrG deficient strain by combining the split-marker strategy and CRISPR-Cas9 technology. Deletion of ligD and pcribo improved gene targeting efficiencies and enabled the use of an additional selection marker in P. crustosum. Furthermore, we reduced the secondary metabolite background by inactivation of two highly expressed gene clusters and abolished the formation of the reactive ortho-quinone methide. Finally, we replaced the P. crustosum pigment gene pcr4401 with the commonly used Aspergillus nidulans wA expression site for convenient use of constructs originally designed for A. nidulans in our P. crustosum host strain. As proof of concept, we successfully expressed a single polyketide synthase gene and an entire gene cluster at the P. crustosum wA locus. Resulting transformants were easily detected by their albino phenotype. With this study, we provide a highly efficient platform for heterologous expression of fungal genes. Key points Construction of a highly efficient Penicillium crustosum heterologous expression host Reduction of secondary metabolite background by genetic dereplication strategy Integration of wA site to provide an alternative host besides Aspergillus nidulans Graphical Abstract

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Brazilian Atlantic Forest and Pampa Biomes in the spotlight: an overview of Aspergillus, Penicillium, and Talaromyces (Eurotiales) species and the description of Penicillium nordestinense sp. nov.

et al. 2022

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The knowledge of the geographical distribution of fungi is essential to promote the understanding of global and national fungal diversity patterns. In this study, we provide a literature-based checklist of Aspergillus, Penicillium, and Talaromyces species recorded in the biomes of the Brazilian Atlantic Forest and the Brazilian Pampa. Only Penicillium digitatum was reported for the Pampa biome. For the Atlantic Forest, a total of 169 species (68 Aspergillus, 79 Penicillium, and 22 Talaromyces) are reported, of which 20 are typified with specimens from this biome, including the novel species Penicillium nordestinense (section Lanata-Divaricata) that is described in this study. Penicillium nordestinense is phylogenetically related to the invalidly described species Penicillium setosum, and this species is validated here. Soil was the most common substrate from where species were reported. Several reported species are well known in biotechnological processes. Penicilliopsis zonata and Sclerocleista ornata, species previously treated in Aspergillus, are recorded. This checklist reflects the limited knowledge of fungal species in tropical environments, such as the Brazilian Atlantic Forest and Pampa biomes. This information is a good framework for understanding the Brazilian diversity of Aspergillus, Penicillium, and Talaromyces and provides data for future fungal biogeographical studies in tropical environments.

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Biochemical Characteristics of Penicillium crustosum FP 11 Xylanase II and an Assessment of the Properties of Xylanases Produced by the Genus Penicillium

Cited by 3 publications

References 33 publications

Isolation and Characterization of Xylanase from a Novel Strain, Penicillium menonorum SP10

Isolation and Characterization of Xylanase from a Novel Strain, Penicillium menonorum SP10

Construction of an expression platform for fungal secondary metabolite biosynthesis in Penicillium crustosum

Brazilian Atlantic Forest and Pampa Biomes in the spotlight: an overview of Aspergillus, Penicillium, and Talaromyces (Eurotiales) species and the description of Penicillium nordestinense sp. nov.

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