Biochemical characteristics of Edwardsiella ictaluri

Waltman, W. D.; Shotts, Emmett B.; Hsu, T C

doi:10.1128/aem.51.1.101-104.1986

Cited by 77 publications

(37 citation statements)

References 5 publications

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“…There was no difference in the profile for the E. ictaluri challenge and recovered strains in this study. The Vietnamese E. ictaluri isolates appear to be slightly less biochemically reactive compared with USA strains (Waltman, Shotts & Hsu 1986). Nevertheless, all E. ictaluri isolates recovered from the affected P. hypophthalmus with clinical signs of BNP had similar identification profiles.…”

Section: Discussionmentioning

confidence: 85%

Experimental challenge studies in Vietnamese catfish, Pangasianodon hypophthalmus (Sauvage), exposed to Edwardsiella ictaluri and Aeromonas hydrophila

Crumlish

Thanh²,

Koesling³

et al. 2010

Journal of Fish Diseases

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The two main diseases in the pangasius catfish industry are bacillary necrosis of Pangasianodon (BNP) and motile aeromonas septicaemia (MAS), where the aetiological agents have been identified as Edwardsiella ictaluri and Aeromonas hydrophila, respectively. In this study, apparently healthy Pangasianodon hypophthalmus were exposed to E. ictaluri, A. hydrophila or both bacterial species by intraperitoneal injection or immersion. There were 20 fish per treatment group, and the bacterial isolates used for the study were recovered from natural infections of BNP or MAS in farmed Vietnamese P. hypophthalmus. The results of the experimental infections mimicked the natural disease outbreaks reported from these pathogens in P. hypophthalmus. Furthermore, it was clearly demonstrated that E. ictaluri was only recovered from the fish exposed to the bacterium and not recovered from the animals receiving A. hydrophila.

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Section: Discussionmentioning

confidence: 85%

Experimental challenge studies in Vietnamese catfish, Pangasianodon hypophthalmus (Sauvage), exposed to Edwardsiella ictaluri and Aeromonas hydrophila

Crumlish

Thanh²,

Koesling³

et al. 2010

Journal of Fish Diseases

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“…Chondroitinase activity was proposed to be one virulence contributor in Edwardsiella spp. (Waltman, Shotts & Hsu 1986) and mediates the cartilage degradation in the chronic 'hole-in-the-head' lesion (Shotts & Cooper 1992).…”

Section: Introductionmentioning

confidence: 99%

Insights into the virulence‐related genes of Edwardsiella tarda isolated from turbot in Europe: genetic homogeneity and evidence for vibrioferrin production

Castro

Osorio

Buján

et al. 2015

Journal of Fish Diseases

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Edwardsiella tarda has long been known as a pathogen that causes severe economic losses in aquaculture industry. Insights gained on E. tarda pathogenesis may prove useful in the development of new methods for the treatment of infections as well as preventive measures against future outbreaks. In this report, we have established the correlation between the presence of virulence genes, related with three aspects typically involved in bacterial pathogenesis (chondroitinase activity, quorum sensing and siderophore-mediated ferric uptake systems), in the genome of E. tarda strains isolated from turbot in Europe and their phenotypic traits. A total of 8 genes were tested by PCR for their presence in 73 E. tarda isolates. High homogeneity was observed in the presence/absence pattern of all the strains. Positive results in the amplification of virulence-related genes were correlated with the detection of chondroitinase activity in agar plates, in vivo AHL production during fish infection and determination of type of siderophore produced by E. tarda. To the best of our knowledge, this is the first study carried out with European strains on potential virulence factors. Furthermore, we demonstrated for the first time that E. tarda produces the siderophore vibrioferrin.

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“…It was first isolated in 1976 from an epizootic in Alabama (Hawke 1979;Hawke et al 1981). Edwardsiella ictaluri is a serologically and biochemical homogeneous species (Hawke 1979;Hawke et al 1981;Waltman et al 1986;Lobb et al 1993), although one study analysing genetic footprints of several isolates found four subgroups (Bader et al 1998).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the rrn operons in the channel catfish pathogen Edwardsiella ictaluri

et al. 2008

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Aims: To advance diagnostics and phylogenetics of Edwardsiella ictaluri by sequencing and characterizing its rrn operons. Methods and results: The Edw. ictaluri rrn operons were identified from a 5–7 kbp insert lambda library and from Edw. ictaluri fosmid clones. We present the complete sequences and analysis of all eight Edw. ictaluri rrn operons and unique regions located upstream and downstream. Two rrn operons were located in tandem with 169 bp separating them, which is apparently a conserved feature between Edw. ictaluri and Edwardsiella tarda. I‐CeuI enzyme digestion of Edw. ictaluri genomic DNA and analysis by pulsed field gel electrophoresis indicated that rrn operon number and chromosomal locations are conserved within the species Edw. ictaluri. Conclusions: The rrn operons of Edw. ictaluri have similar structure and flanking regions compared with other members of the family Enterobacteriaceae; however, the presence of eight copies of the rrn operon makes Edw. ictaluri unique within the family. Significance and impact of the study: This research clarifies previous phylogenetic analyses of Edw. ictaluri and provides support for the Edw. ictaluri genome sequencing project. In addition, we identified a unique feature of two rrn operons that shows potential for the development of a diagnostic PCR method.

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Biochemical characteristics of Edwardsiella ictaluri

Cited by 77 publications

References 5 publications

Experimental challenge studies in Vietnamese catfish, Pangasianodon hypophthalmus (Sauvage), exposed to Edwardsiella ictaluri and Aeromonas hydrophila

Experimental challenge studies in Vietnamese catfish, Pangasianodon hypophthalmus (Sauvage), exposed to Edwardsiella ictaluri and Aeromonas hydrophila

Insights into the virulence‐related genes of Edwardsiella tarda isolated from turbot in Europe: genetic homogeneity and evidence for vibrioferrin production

Characterization of the rrn operons in the channel catfish pathogen Edwardsiella ictaluri

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