Biochemical characteristics of collagen produced by long term cultivated amniotic fluid cells

Hurych, J; Maçek, Milan; Beniač, F.; Rezácová, D

doi:10.1007/bf00270863

Cited by 15 publications

(8 citation statements)

References 16 publications

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“…However, that study did not specify the identity of these cells. The presence of mesenchymal cells in the amniotic fluid has been suggested for decades [35,36]. Still, the progenitor identity of mesenchymal amniocytes started to be determined only very recently [21,37,38].…”

Section: Discussionmentioning

confidence: 99%

Tissue engineering from human mesenchymal amniocytes: a prelude to clinical trials

Kunisaki

Armant

Kao

et al. 2007

Journal of Pediatric Surgery

113

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Section: Discussionmentioning

confidence: 99%

Tissue engineering from human mesenchymal amniocytes: a prelude to clinical trials

Kunisaki

Armant

Kao

et al. 2007

Journal of Pediatric Surgery

113

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“…All epithelial surface cells that are in direct or indirect contact with the amniotic fluid have been considered as the sources of such cells including fetal epidermis, the epithelial cells of the respiratory, the urinary, and the intestinal tract of the fetus, and the epithelium of the "amniotic membrane" (Gerbie et al 1972;Hoehn et al 1975). The occurrence of cells with fibroblast-like morphology and detection of collagen type I in these cultures suggest that some of the clonable cells are true flbroblasts which stem from the connective tissue underlying the epithelial layers mentioned above (Macek et al 1973;Priest et al 1977;Hurych et al 1976).…”

Section: Introductionmentioning

confidence: 99%

Characterization of cells of amniotic fluids by immunological identification of intermediate-sized filaments: Presence of cells of different tissue origin

et al. 1981

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“…Addendum During preparation of this manuscript, there appeared a report (Hurych et al, 1976) presenting studies performed on one amniotic fluid culture 'after 19 weeks of cultivation'. From a determination of the chain ratio, the authors concluded that this culture synthesized type I collagen, and they then discussed possible applications to prenatal investigation of inherited disorders of collagen.…”

mentioning

confidence: 99%

Differentiation in human amniotic fluid cell cultures: I: Collagen production.

Priest¹,

Priest²,

Moinuddin³

et al. 1977

Journal of Medical Genetics

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SUMMARYThe collagen produced by differentiated cells cultured from human amniotic fluid was characterized in two ways. By chain composition and by 4-hydroxyproline: 3-hydroxyproline isomer ratio, the collagen synthesized by F-type (fibroblast) cells was indistinguishable from that made by cultured fetal dermal fibroblasts. The predominant cells in young amniotic fluid cultures, termed AF-type, produced collagen with a lower isomer ratio, resembling that of basement membrane collagen. The chain composition, as determined by chromatography on carboxymethyl cellulose, varied for different cultures of the AF-type, but the major pattern was consistent with that of basement membrane collagen. On the basis of these characteristics, F cells are of fibroblast origin, whereas most AF cells are of a different origin either endothelial or epithelial. Other evidence (Megaw et al., 1977) suggests an epithelial origin for AF cells.Studies using human amniotic fluid cell culture as an aid to prenatal diagnosis are increasing in complexity. We began to investigate collagen synthesized by these cell cultures because of the need for prenatal diagnosis of inherited diseases affecting collagen. Initial studies in this laboratory had shown increased solubility of collagen produced by cultured dermal fibroblasts from Marfan syndrome compared with controls (Priest et al., 1973), and the question arose whether this or other disorders of connective tissue could be diagnosed in cultured amniotic fluid cells. Therefore, further studies were performed on collagen laid down in the cell layer and extracted by acid or neutral salt, or on collagen released into culture medium by two types of fetal cells cultured from amniotic fluid, F (fibroblast) and AF (amniotic fluid) types (Hoehn et al., 1974).We report here that the F and AF cells, distinguished from each other originally on the basis of morphology and growth characteristics, also differ biochemically in the types of collagen formed in culture. We also report another biochemical difference (Megaw et al

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Biochemical characteristics of collagen produced by long term cultivated amniotic fluid cells

Cited by 15 publications

References 16 publications

Tissue engineering from human mesenchymal amniocytes: a prelude to clinical trials

Tissue engineering from human mesenchymal amniocytes: a prelude to clinical trials

Characterization of cells of amniotic fluids by immunological identification of intermediate-sized filaments: Presence of cells of different tissue origin

Differentiation in human amniotic fluid cell cultures: I: Collagen production.

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