Biochemical changes in phosphorus compounds and in the activity of phytase and α‐amylase in the rice (Oryza sativa) grain during germination

Abstract

Changes in the phytic acid, inorganic phosphorus and A T P contents, and in the activity of phytase and a-amylase in rice (

Effect of germination on the phytase activity, phytate and total phosphorus contents of rice (Oryza sativa), maize (Zea mays), millet (Panicum miliaceum), sorghum (Sorghum bicolor) and wheat (Triticum aestivum)

“…A similar trend was reported for germinating rice (Kikunaga et al 1991), lentil (Greiner 2002), barley (Sung et al 2005) and soybean (Prazeres et al 2004). While a maximum of 16-fold increase in phytase activity was observed for rice grains in this study, Kikunaga et al (1991) reported a maximum of 7-fold increase also after germinating rice grains for 10 days. Sung et al (2005) reported that germination temperature affects intrinsic phytase acitivity.…”

“…After this increase, phytase activity declined slightly (P<0.05) probably due to the degradation of the enzyme by active protease (Houde et al 1990) or due to inhibition by accumulating phosphate (Sung et al 2005). A similar trend was reported for germinating rice (Kikunaga et al 1991), lentil (Greiner 2002), barley (Sung et al 2005) and soybean (Prazeres et al 2004). While a maximum of 16-fold increase in phytase activity was observed for rice grains in this study, Kikunaga et al (1991) reported a maximum of 7-fold increase also after germinating rice grains for 10 days.…”

Effect of salinity on germination, phytase activity and phytate content in lettuce seedling

“…Seed phytates constitute a Pi source during the early stages of germination when phosphate supply is a limiting factor or when there is no other phosphate source for this process. Phosphate is released from phytate through the enzymatic action of phytases that are abundant in dry seeds and increases rapidly during germination (Kikunaga et al 1991). Centeno et al (2001) observed an increase of phytase activities with a concomitant decrease of phosphorus phytate and an increase in the content of inositol phosphates during the rye and barley germination.…”

Effects of phytase and polyphenol oxidase treatments onin vitroiron bioavailability in faba bean (Vicia faba L.)

“…Moreover, the germination process itself significantly reduced the phytate content in faba bean (Table 1), which may be due to activation of endogenous phytase. Activation of phytase during germination has been shown in several cereals (Bartnik & Szafranska, 1987;Greiner, Jany, & Larsson Alminger, 2000;Kikunaga, Katoh, & Takahashi, 1991), as well as in legumes (Alonso, Aguirre, & Marzo, 2000). …”