Biochemical changes during larval development in the short neck clam,<i>Paphia malabarica</i>Chemnitz

Raghavan, G.S.V.; Biju, Abraham; Muthiah, P

doi:10.1111/j.1365-2109.2009.02251.x

Cited by 8 publications

(4 citation statements)

References 22 publications

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“…Prior to attaining feeding competency, lipids are the most important sources of energy during embryonic and larval development for many bivalve species [ 194 , 195 , 196 ]. When energy requirements are high, lipases hydrolyse triacylglycerols and release fatty acids (FAs).…”

Section: Discussionmentioning

confidence: 99%

Metabolic Regulation of Copper Toxicity during Marine Mussel Embryogenesis

et al. 2023

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The development of new tools for assessing the health of cultured shellfish larvae is crucial for aquaculture industries to develop and refine hatchery methodologies. We established a large-volume ecotoxicology/health stressor trial, exposing mussel (Perna canaliculus) embryos to copper in the presence of ethylenediaminetetraacetic acid (EDTA). GC/MS-based metabolomics was applied to identify potential biomarkers for monitoring embryonic/larval health and to characterise mechanisms of metal toxicity. Cellular viability, developmental abnormalities, larval behaviour, mortality, and a targeted analysis of proteins involved in the regulation of reactive oxygen species were simultaneously evaluated to provide a complementary framework for interpretative purposes and authenticate the metabolomics data. Trace metal analysis and speciation modelling verified EDTA as an effective copper chelator. Toxicity thresholds for P. canaliculus were low, with 10% developmental abnormalities in D-stage larvae being recorded upon exposure to 1.10 μg·L−1 bioavailable copper for 66 h. Sublethal levels of bioavailable copper (0.04 and 1.10 μg·L−1) caused coordinated fluctuations in metabolite profiles, which were dependent on development stage, treatment level, and exposure duration. Larvae appeared to successfully employ various mechanisms involving the biosynthesis of antioxidants and a restructuring of energy-related metabolism to alleviate the toxic effects of copper on cells and developing tissues. These results suggest that regulation of trace metal-induced toxicity is tightly linked with metabolism during the early ontogenic development of marine mussels. Lethal-level bioavailable copper (50.3 μg·L−1) caused severe metabolic dysregulation after 3 h of exposure, which worsened with time, substantially delayed embryonic development, induced critical oxidative damage, initiated the apoptotic pathway, and resulted in cell/organism death shortly after 18 h of exposure. Metabolite profiling is a useful approach to (1) assess the health status of marine invertebrate embryos and larvae, (2) detect early warning biomarkers for trace metal contamination, and (3) identify novel regulatory mechanisms of copper-induced toxicity.

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Section: Discussionmentioning

confidence: 99%

Metabolic Regulation of Copper Toxicity during Marine Mussel Embryogenesis

et al. 2023

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“…The scallop Placopecten magellanicus showed no significant variation in total lipid content from the time of spawning to 8 days postfertilization, with decreasing levels of triacylglycerol, whereas free fatty acids, cholesterol, acetone mobile polar lipids and phospholipids increased significantly (Pernet, Tremblay & Bourget ). The utilization of lipids as the dominant energy reserve in eggs was reported for Paphia malabarica (Gireesh, Biju & Muthiah ) and Argopecten irradians (Lu, Blake & Torres ). However, Crassadoma gigantea and Patinopecten yessoensis showed a decrease in lipids and proteins in the same proportion, but to a lesser extent for carbohydrates (Whyte et al .…”

Section: Discussionmentioning

confidence: 81%

Biochemical and fatty acid dynamics during larval development in the razor clamEnsis arcuatus(Bivalvia: Pharidae)

et al. 2012

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This article reports on the changes in gross biochemical and fatty acid composition of the razor clam Ensis arcuatus larvae throughout development. Protein was the largest component of dried larval tissues. The energy required for embryogenesis in E. arcuatus oocytes was obtained from stored proteins and carbohydrates, while total lipids increased significantly. Lipids and carbohydrates have an important role as energy contributors from day 1 to day 8. During larval development the strategy was to indistinctly store energy reserves (protein, lipid and carbohydrate) to surpass metamorphosis. During embryonic development there was a gain in fatty acids of neutral and polar lipids. A depletion of fatty acids in neutral lipids was observed from day 1 to day 8 in E. arcuatus larvae, mainly due to the decrease in 16:0 and EPA. NMID fatty acids were present in amounts similar to those of some PUFAs in polar lipids, especially 22:2NMID.

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“…Spearman' Correlation Analysis between gonadal dry biomass and biochemical components (proteins, carbohydrates and lipids) in each of the tissues tested (other tissue, muscle, and gonads) of Arca zebra (n = 10 months) utilizó las proteínas como sustrato energético durante su actividad reproductiva, ya que los incrementos de la masa seca de las gónadas venían acompaña-dos simultáneamente por descensos de las proteínas (septiembre y diciembre de 2010, mayo de 2011). En este sentido, se ha demostrado que las proteínas, aparte de constituir uno de los principales componentes de los ovocitos en bivalvos (Gireesh et al 2009), pueden aportar energía durante el proceso gametogénico, después de los carbohidratos y los lípidos de reserva (Barber & Blake, 1981), lo que podría significar que las proteínas fueron utilizadas como una fuente importante de energía durante la gametogénesis en A. zebra, tal y como ha sido reportado para otras especies, tales como: Crassostrea gigas (Li et al 2000), Crassostrea plicatula (Li et al 2006) y Sinonovacula constricta (Yan et al 2010). La contribución de las proteínas procedentes del músculo durante la gametogénesis también se ha reportado para Mytilus edulis (Gabbott & Bayne, 1973).…”

Section: Discussionunclassified

Movilización y utilización de los sustratos energéticos de <i>Arca zebra</i> (Bivalvia: Arcidae) en relación con el ambiente

2016

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RESUMENEl análisis de los componentes bioquímicos en especies marinas para el consumo humano tiene una gran importancia, ya que permite conocer las propiedades alimenticias y energéticas del organismo. En el presente estudio, se evaluó el perfil bioquímico en tejidos de Arca zebra en relación con los factores ambientales, con la finalidad de ser utilizado como indicador de la dinámica reproductiva y de los periodos de engorde de este importante recurso pesquero. Se analizaron 20 organismos/ mes, entre julio de 2010-julio de 2011. A cada organismo se le determinó el largo total (LT) y se separaron los tejidos, los cuales se llevaron hasta peso constante para realizarles los análisis bioquímicos mediante espectrofotometría. Se establecieron diferencias mensuales en la LT, con un promedio anual de 69.61 mm, manteniéndose por encima de la talla comercial. Los carbohidratos y las proteínas mostraron diferencias entre meses, con valores máximos en marzo y julio de 2011, siendo el músculo quien registró el mayor contenido de ambos sustratos energéticos, asociados a los altos niveles de clorofila a. Se observó una relación inversa entre los carbohidratos y las proteínas en los tres tejidos con respecto a la masa gonadal, sugiriendo una posible transferencia durante el proceso reproductivo de A. zebra. En las gónadas se registró el mayor contenido de lípidos, sin variación mensual, debido a la sincronía reproductiva de la especie. A. zebra muestra su mejor condición nutricional durante los periodos comprendidos entre septiembre-diciembre y marzo-junio, en estos meses se recomienda su comercialización e ingesta.Palabras claves: Arcidae, componentes bioquímicos, factores ambientales (temperatura, seston, Cl a), Venezuela, reproducción. ABSTRACTThe analysis of biochemical components in marine species for human consumption is of great importance because it allows knowing the nutritional and energy properties of the organism. The biochemical profile of Arca zebra tissues was evaluated in this research in relation to environmental factors, with the purpose of using this information as an indicator of the reproductive dynamics and fattening periods of this important fishery resource. A total of 20 organisms were analyzed per month between July 2010 and July 2011. Total length (TL) was determined in each organism, and tissues were separated using spectrophotometry, leaving them at a constant weight

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Biochemical changes during larval development in the short neck clam,Paphia malabaricaChemnitz

Cited by 8 publications

References 22 publications

Metabolic Regulation of Copper Toxicity during Marine Mussel Embryogenesis

Metabolic Regulation of Copper Toxicity during Marine Mussel Embryogenesis

Biochemical and fatty acid dynamics during larval development in the razor clamEnsis arcuatus(Bivalvia: Pharidae)

Movilización y utilización de los sustratos energéticos de <i>Arca zebra</i> (Bivalvia: Arcidae) en relación con el ambiente

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