Biochemical Changes During In Vitro Organogenesis of Tylophora indica (Burm. F.) Merrill

Rathod, D. R.; Patel, Asha; Shrimali, Gaurav; Rami, Esha; Patel, C. H.; Panigrahi, Jitendriya; Patel, Illa

doi:10.15373/2249555x/jan2014/79

Cited by 8 publications

(8 citation statements)

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“…Ascorbic acid is a strong antioxidant, controls the color pigmentation of the embryogenic callus and is involved in plant antioxidant system to cope any stress condition (Khan et al 2015b ). Additionally ascorbic acid has long been recognized as a strong antioxidant for its role in oxidative phosphorylation and photophosphorylation, stimulation of RNA synthesis, bud development and prevention of senescence (Rathod et al 2012 ).…”

Section: Resultsmentioning

confidence: 99%

Evaluation of biochemical markers during somatic embryogenesis in Silybum marianum L.

et al. 2016

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In present report effects of explants type, basal media and plant growth regulators (PGRs) were tested for induction of indirect somatic embryogenesis in medicinally important plant Silybum marianum L. Leaf, petiole and root explants were exploited in vitro on B5 (Gamborg), SH (Schenk and Hildebrandt) and MS (Murashige and Skoog) media for induction of embryogenic callus followed by somatic embryogenesis. Highest callus induction frequency (76 ± 4.8 %) was recorded when petiole explants of in vitro derived plantlets were cultured on B5 medium supplemented with 1.5 mg l−1 2,4-dichlorophenoxyacetic acid (2,4_D) in combination with 1.5 mg l−1 Thidiazuron (TDZ). Induction and multiplication of somatic embryos were observed, when the embryogenic calluses were sub-cultured on to B5 medium containing 0.5 mg l−1 2,4-D plus 1.5 mg l−1 TDZ. At this PGRs treatment, 77 % of the cultures responded with 39.1 somatic embryos per callus. Furthermore, MS0 medium was indicated more reponsive for growth and maturation of somatic embryos. Analysis of biochemical markers during various growth phases in somatic embryogenesis revealed that somatic embryos exhibited highest level of total carbohydrate, starch, ascorbic acid and total free amino acids. However, higher protein levels were detected in non-embryogenic callus. Nevertheless, considerable amount of silymarin (4.1 mg g−1 DW) was detected in somatic embryos than other growth phases. Thus, the present study concluded that biochemical and physiological changes during embryogenesis are influenced by interplay of explants type, basal media and PGRs.

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Section: Resultsmentioning

confidence: 99%

Evaluation of biochemical markers during somatic embryogenesis in Silybum marianum L.

et al. 2016

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“…These phenolic acids are produced as intermediary products of phenylpropanoid metabolic pathway, and can be anticipated for stimulating role in biomass formation during in vitro root culture in present study. They are also reported for their role in oxidative phosphorylation and photophosphorylation, stimulation of RNA synthesis, bud development as well as prevention of senescence due to their strong antioxidant nature (Rathod et al 2014). Both cinnamic acid and di-hydro kaempferol activate antioxidant enzymes SOD and POD against reactive oxygen species (ROS), under stress conditions (Szalai and Janda 2009).When taken into account total ion counts (Fig.…”

Section: Evaluation Of Key Metabolites In Different Growth Stages Durmentioning

confidence: 99%

Analysis of metabolic variations throughout growth and development of adventitious roots in Silybum marianum L. (Milk thistle), a medicinal plant

Khan

Abbasi

Shah

et al. 2015

Plant Cell Tiss Organ Cult

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Silybum marianum L. is a medicinal plant used in the treatment for jaundice and liver diseases. In this study, an adventitious root culture was developed for the production of health promoting phytochemicals. Adventitious roots were induced from nodal explants on solid Murashige and Skoog medium supplemented with 1.0 mg l -1 of a-Naphthalene acetic acid. Growth kinetics of the roots was investigated every week, for 8 weeks of culture period. Highest fresh biomass formation (153 mg l -1 ) was observed in 6-week old cultures. Adventitious roots were harvested from different growth stages as control (CTR), lag phase (LAG), logarithmic phase (LOG) or stationary phase (STN). Metabolite profiling of the samples was investigated using electro spray ionization time of flight mass spectrometry. Significant phenylpropanoids such as cinnamic acid and di-hydro kaempferol were predominantly found in LOG phase, whereas the highest amount of malonic acid was detected in STN as compared to other growth phases. More sucrose content was detected in CTR, while the tryptophan content was higher in LOG phase. Among the vital fatty acids, prostaglandin A1 and phenyl acetic acid were at highest levels in STN phase. However, more brassicasterols were observed in LAG phase than other growth phases. Punicic acid and lignan pinoresinol were detected abundantly in the LOG phase. Biochemical characterization revealed significant correlations between silymarin content and DPPH as well as TPC and TFC in the growth curve. Interestingly, among all growth stages there was no correlation of PAL activity with TFC and silymarin content.

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“…Shoot, leaf, nodal segments, green and white root segments as well as petiole were used as explants. Amid all the explants used for organogenesis or embryogenesis, it was notably detected and reported that leaf was the most widely used explant source (Jayanthi and Mandal 2001;Singh et al 2009aSingh et al , 2010Anand et al 2012;Koilpillai 2012;Jahan et al 2013;Chaturvedi and Chowdhary 2013;Sadguna et al 2013;Rathod et al 2014;Sharma et al 2014) that effectively exhibited adventitious multiple shoots (Kaur et al 2011a;Nayeem et al 2014), calli, or somatic embryo formation. Reddy et al (2010) reported the use of leaves that confirmed even induction of in vitro rooting.…”

Section: Source Of Explants and Their Reaction In Vitromentioning

confidence: 99%

“…The type of sterilant and the duration of explants' exposure to the sterilant are the two crucial factors of disinfection. The explants collected from fields were washed properly for 10-30 min under running tap water followed by treatment with 0.1% (w/v) Bavistin Ò (fungicide) and 5% Teepol TM (v/v) (mild detergent) solution for 5 min, and again thoroughly washed with sterile distilled water Anand et al 2012;Kaushik et al 2010;Rathod et al 2014). Haque and Ghosh (2013) accounted the use of TWEEN Ò 20 for 3 min for sterilizing young leaves of Tylophora.…”

Section: Surface Disinfectionmentioning

confidence: 99%

“…As temperature influences the rate of photosynthesis and respiration (rapidly increases with the rise in temperature), maintaining an ambient temperature required for suitable in vitro regeneration is another vital physical factor. The temperature of the growth room was kept at 25 ± 2°C by the researchers, whose main aim was to induce shoot buds from the plant material (Faisal et al , 2010Reddy et al 2010;Kaur et al 2011a;Anjum et al 2014;Nayeem et al 2014;Rajavel and Stephan 2014a, b;Soni et al 2015b), as well as callus from explants (Kaushik et al 2010;Sahai et al 2010a,b;Kaur et al 2011b, c;Anand et al 2012;Sadguna et al 2013;Rathod et al 2014;Soni et al 2015a;Jogdand et al (2016), or even somatic embryogenesis (Manjula et al 2000;Chandrasekhar et al 2006;Sahai et al 2010a). Multiple shoot regeneration was reported by Rathore et al (2010) by allowing the nodal shoot segments to grow under a bit higher temperature 27 ± 2°C.…”

Section: Influence Of Physical Factorsmentioning

confidence: 99%

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Neoteric trends in tissue culture-mediated biotechnology of Indian ipecac [Tylophora indica (Burm. f.) Merrill]

Gantait

Kundu

2017

3 Biotech

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Tylophora indica (Burm. f.) Merrill, an ethno-pharmacologically important perennial climber of Asclepiadaceae, is commonly known as Antamul or Indian ipecac. It is essentially accredited for its medicinal properties owing to its wide range of alkaloids in the form of bioactive secondary metabolites, such as tylophorine, tylophorinine, and tylophorinidine. Accelerated mass propagation of Tylophora is challenging because of its reduced seed germination frequency that consequently headed the pursuit for efficient protocols on in vitro propagation for the large-scale regeneration, conservation as well as sustainable supply of quality propagules. Ample tissue culture-mediated biotechnological investigations have been carried out on this medicinal plant till date and several micropropagation protocols have been standardized as well. The present review compares between several typical methods as well as factors, involving on direct and indirect organogenesis of Tylophora along with various up-to-date and modified techniques such as somatic embryogenesis, protoplast culture, synthetic seed production, genetic transformation, and in vitro interventions for the secondary metabolite production that have been reported in last two decades. This compilation will allow assessing the achievements and trends of Tylophora research so far, as well as will advance the research more rapidly, since many aspects, basic and applied, have yet to be explored.

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Biochemical Changes During In Vitro Organogenesis of Tylophora indica (Burm. F.) Merrill

Cited by 8 publications

References 0 publications

Evaluation of biochemical markers during somatic embryogenesis in Silybum marianum L.

Evaluation of biochemical markers during somatic embryogenesis in Silybum marianum L.

Analysis of metabolic variations throughout growth and development of adventitious roots in Silybum marianum L. (Milk thistle), a medicinal plant

Neoteric trends in tissue culture-mediated biotechnology of Indian ipecac [Tylophora indica (Burm. f.) Merrill]

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