Biochemical Basis for Effects of K-Deficiency on Assimilate Export Rate and Accumulation of Soluble Sugars in Soybean Leaves

Huber, Steven C.

doi:10.1104/pp.76.2.424

Cited by 105 publications

(68 citation statements)

References 26 publications

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“…On the contrary, it was distributed throughout the whole plant tissues, especially petioles, under high K. Growth of many plants on limited K supply has previously been demonstrated to lead to large changes, increase or decrease, in primary metabolites; e.g. strong increase in soluble sugars in leaves of Arabidops (Armengaud et al 2009), bean (Cakmak et al 1994a(Cakmak et al , 1994b, cotton (Bednarz and Oosterhuis, 1999;Pettigrew, 1999), gentian (Takahashi et al 2012), soybean (Huber, 1984) and wheat (Ward, 1960), alfalfa (Li et al 1997), sugar beet (Farley and Draycott, 1975), and tomato (Urbanczyk-Wochniak and Fernie, 2005;Sung et al, 2015). However, the information reported from previous studies was mostly focused on the specific tissues such as leaves and roots and mineral deficient conditions.…”

Section: Discussionmentioning

confidence: 99%

Tissue-specific response of primary metabolites in tomato plants affected by different K nutrition status

Sung¹,

Yun²,

Cho³

et al. 2017

Plant Omics

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As one of the most important mineral nutrient elements, potassium (K) plays crucial roles in many fundamental processes, including enzyme activation, membrane transport, anion neutralization and osmo-regulation, and determines the yield and quality of crop production. In order to better understand and elucidate plant tissue-specific primary metabolic changes under different K nutrition status. Four-week-old tomato plants were subjected to different K nutrition situations: low (0.25 mM); normal (2.5 mM); and high (10.0 mM); and the emerging leaves, fully expanded leaves, petioles, stem and roots were harvested at 15 and 30 days, time points which the external symptoms are observed, after K treatments. Primary metabolites, amino acids, organic acids and sugars, extracted from each tomato tissue were measured with HPLC system. Several interesting findings from this study could be summarized as follows: (1) metabolites showed K-dependent responses, which indicated that the rates of an increase and decrease in low K-affected were 50 % : 50 % ;whereas, 80 % : 20 % in high K; (2) the petioles revealed the most sensitive plant tissue in response to K nutrition status; and (3) metabolites such as glucose and fructose (soluble sugars), malate and citrate (organic acids), and glutamine, asparagine, glutamate and aspartate (amino acids) strongly fluctuated (up or down) by the K nutrition ratio. These findings may contribute to a better understanding and elucidating the tissue-specific biosynthetic patterns and primary metabolite accumulation under different K nutrition ratios, and provide a new strategy for comprehensive information involved in the spatio-temporal metabolic networks

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Section: Discussionmentioning

confidence: 99%

Tissue-specific response of primary metabolites in tomato plants affected by different K nutrition status

Sung¹,

Yun²,

Cho³

et al. 2017

Plant Omics

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“…Insoluble matter was removed by centrifugation at 38,000g for 10 min and an aliquot of the extract was desalted by passage through a small column of Sephadex G-25. Assay procedures for SPS (23), cytoplasmic fructose bisphosphatase (23), starch synthase (5), UDP-glucose pyrophosphorylase (27), ADP-glucose pyrophosphorylase (27), and invertase (13) (10). This may represent a case of a single locus gene duplication as has been suggested for anomalous isozyme patterns in other species (10).…”

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confidence: 98%

Alterations in Growth, Photosynthesis, and Respiration in a Starchless Mutant of Arabidopsis thaliana (L.) Deficient in Chloroplast Phosphoglucomutase Activity

Caspar¹,

Huber²,

Somerville³

1985

Plant Physiol.

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497

561

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(12,23,28).The effect of carbohydrate accumulation on the rate of dark respiration has received less attention but appears to have been consistently observed by a variety of approaches (2,6,29). The essential observation is that the rate of respiration is proportional to carbohydrate content or is stimulated by provision of exogenous carbohydrate. The implication is that the amount of respiration is regulated by substrate supply rather than demand for ATP or reducing equivalents. Indeed, it has been suggested that, under conditions of high carbohydrate supply, a substantial proportion of the reductant generated during carbohydrate catabolism may be consumed by the alternative oxidase without being linked to ATP production (3,15

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“…The latter quantity is often estimated from increases in leaf mass, assuming that all the increased mass is carbohydrate of the formula CH2O, i.e. 40% C (4,8,(10)(11)(12)21).…”

Section: Abstracitmentioning

confidence: 99%

“…4, [10][11][12]22). Because environmental changes alter the mix of organic and inorganic compounds accumulating in leaves, the assumption of constant C concentration between environments needs verification.…”

mentioning

confidence: 99%

Carbon Accumulation during Photosynthesis in Leaves of Nitrogen- and Phosphorus-Stressed Cotton

Radin¹,

Eidenbock²

1986

Plant Physiol.

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ABSTRACITLeaves of cotton (Gossypium hirsutum L.) accumulate considerable dry mass per unit area during photosynthesis. The percentage of C in that accumulated dry mass was estimated as the regression coefficient (slope) of a linear regression relating C per unit area to total dry mass per unit area. Plants were grown on full nutrients or on N-or P-deficient nutrient solutions. In the fully nourished controls, the mass that accumulated over a 9-hour interval beginning at dawn contained 38.6% C. N and P stress increased the C concentration of accumulated mass to 49.7% and 45.1%, respectively. Nutrient stress also increased the starch concentration of accumulated mass, but starch alone could not account for the differences in C concentration. P stress decreased the estimated rate of C export from source leaves, calculated as the difference between C assimilation and C accumulation. The effect of P stress on apparent export was very sensitive to the C concentration used in the calculation, and would not have been revealed with an assumption of unchanged C concentration in the accumulated mass.In recent years much interest has developed in C balances of photosynthesizing leaves, primarily for the purpose ofcalculating rates of assimilate export (4, 8-12, 21, 22, 24). In a mature (nonexpanding) leaf, export can be estimated simply as the difference between C gained by photosynthesis and C accumulated in the leaf. The latter quantity is often estimated from increases in leaf mass, assuming that all the increased mass is carbohydrate of the formula CH2O, i.e. 40% C (4,8,(10)(11)(12)21).Although conceptually simple and theoretically applicable under all circumstances, such C balances must be interpreted with caution when applied to plants under different growth conditions (e.g. 4, 10-12, 22). Because environmental changes alter the mix of organic and inorganic compounds accumulating in leaves, the assumption of constant C concentration between environments needs verification. Of the studies listed above, only two (9, 22) reported direct C analyses of leaf tissue. Actual leaf C concentrations can vary greatly from 40% (e.g. 9). Errors in estimating C accumulation can assume great proportions because the export rate is sometimes much smaller than accumulation (8,21,22). Here we report large effects of inorganic nutrition (N and P status) on C concentration of accumulated dry mass in cotton leaves, and show how these effects alter calculations of assimilate export rates. MATERIALS AND METHODSCotton (Gossypium hirsutum L. cv. Deltapine 70) plants, grown from seed in pots in a glasshouse as described earlier (15,20), were watered three times per week with a modified halfstrength Hoagland solution. N and P deficiencies were imposed by altering the solution composition to contain 20% of the normal amount of N or no added P, respectively. Normal concentrations of N (as NO3-) and P (as H2PO4-) were 5 mM and 0.5 mm, respectively. All measurements were made on fully expanded leaves of vegetative plants in full sunlight on clear da...

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Biochemical Basis for Effects of K-Deficiency on Assimilate Export Rate and Accumulation of Soluble Sugars in Soybean Leaves

Abstract: ABSTRACrThe effects of K-deficiency on carbon exchange rates (CER), photosynthate partitioning export rate, and activities of key enzymes involved in sueme metabolism were studied in soybean (

Cited by 105 publications

References 26 publications

Tissue-specific response of primary metabolites in tomato plants affected by different K nutrition status

Tissue-specific response of primary metabolites in tomato plants affected by different K nutrition status

Alterations in Growth, Photosynthesis, and Respiration in a Starchless Mutant of Arabidopsis thaliana (L.) Deficient in Chloroplast Phosphoglucomutase Activity

Carbon Accumulation during Photosynthesis in Leaves of Nitrogen- and Phosphorus-Stressed Cotton

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