Biochemical Assessments of Seminal Plasma Zinc, Testis-Expressed Sequence 101 and Free Amino Acids and Their Correlations with Reproductive Hormones in Male Infertility

Saleem, Tahia H.; Okasha, Marwa; Ibrahim, Hassan; El‐Hamd, Mohammed Abu; Fayed, Hanan Mahmoud; Hassan, Mohammed H.

doi:10.1007/s12011-020-02310-9

Cited by 12 publications

(10 citation statements)

References 50 publications

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“…The biochemical assessment of amino acids in SP among 50 azoospermic, 50 OAT and 50 healthy control samples [ 54 ] was performed using an S433 amino acid analyzer and an HPLC-based system from Sykam GmbH (Eresing, Germany). This revealed significantly lower median concentrations of Ala, Arg, Asn, Asp, Gly, His, Ile, Leu, Lys, Orn, Pro, Ser, Thr, Trp, Tyr and Val in azoospermic men compared to the other two groups.…”

Section: Analytical Methods Used To Study the Human Semen Metabolomementioning

confidence: 99%

Metabolomics of Human Semen: A Review of Different Analytical Methods to Unravel Biomarkers for Male Fertility Disorders

Blaurock

Baumann

Grunewald

et al. 2022

IJMS

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Background: Human life without sperm is not possible. Therefore, it is alarming that the fertilizing ability of human spermatozoa is continuously decreasing. The reasons for that are widely unknown, but there is hope that metabolomics-based investigations may be able to contribute to overcoming this problem. This review summarizes the attempts made so far. Methods: We will discuss liquid chromatography–mass spectrometry (LC-MS), gas chromatography (GC), infrared (IR) and Raman as well as nuclear magnetic resonance (NMR) spectroscopy. Almost all available studies apply one of these methods. Results: Depending on the methodology used, different compounds can be detected, which is (in combination with sophisticated methods of bioinformatics) helpful to estimate the state of the sperm. Often, but not in all cases, there is a correlation with clinical parameters such as the sperm mobility. Conclusions: LC-MS detects the highest number of metabolites and can be considered as the method of choice. Unfortunately, the reproducibility of some studies is poor, and, thus, further improvements of the study designs are needed to overcome this problem. Additionally, a stronger focus on the biochemical consequences of the altered metabolite concentrations is also required.

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Section: Analytical Methods Used To Study the Human Semen Metabolomementioning

confidence: 99%

Metabolomics of Human Semen: A Review of Different Analytical Methods to Unravel Biomarkers for Male Fertility Disorders

Blaurock

Baumann

Grunewald

et al. 2022

IJMS

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“…In parallel, we could map several proteins like Testis-expressed sequence 10-like protein (tex10), Tudor domain-containing 5-like protein (tdrd5), F-box only 22-like protein, Glomulin and ATP-dependent RNA helicases predominantly in male gonad. Tex10 is a membrane anchored protein reported to be involved in male fertility and acrosome reaction 35 . Tdrd5 is reported to be involved in male reproductive functions 36 .…”

Section: Landscape Of Protein Expression Across the Organsmentioning

confidence: 99%

“…However, number of phosphorylated peptides and canonical phosphoproteins were found highest in brain (227, 139) followed by spinal cord (132, 69), heart (115, 81), male gonad (103, 52), kidney (102,77), eye (91, 76), muscle (89,40), liver (87, 63), skin (54,42), female gonad (54,33), air bladder (53,27), gill (38,35), gut (34,28), gall bladder (16,18), spleen (15, 13), fin (13, 11), embryo (11,10), plasma (5, 2) and scales (4, 3). Overall, Serine phosphorylation was found to be predominant across the organs (Fig.…”

Section: Extensive Ptm Catalogue Of L Rohitamentioning

confidence: 99%

The Proteome Map and PeptideAtlas of a widely cultivated tropical water fish Labeo rohita: A resource for the Aquaculture Community

Nissa

Reddy

Mukherjee

et al. 2021

Preprint

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With the global consumption of fish outpacing population growth, aquaculture sector is facing challenges to address the rising demand of food and nutritional security. Integrative omics research provides a strong platform to understand the basic biology and translate this knowledge into sustainable solutions in tackling disease outbreak, increasing productivity thus ensuring food security. To further understand the complex biology of host-pathogen response and support the aquaculture effort, genome and proteome reference maps moving beyond simple sequence information of cultivated fish species will accelerate research and translation of quality products for food industries. Towards this end, we have performed an extensive proteomics-based investigation of Labeo rohita, one of the economically important fish species produced in world aquaculture. Deep proteomic profiling of 17 histologically normal tissues, plasma and embryo provided mass-spectrometric evidence for 6015 high confident canonical proteins at 1% false discovery rate. Tissue enriched expression of several biologically important proteins was validated using targeted proteomics with high quantitative accuracy. We characterised the global post translational modifications (PTMs) in terms of acetylation (n-terminus and lysine), methylation (n-terminus, lysine and arginine) and phosphorylation (serine, threonine and tyrosine) to present a comprehensive proteome resource. An interactive web-based portal was developed to support the Labeo rohita PeptideAtlas, (www.peptideatlas.org), a unique community resource for mass spectrometry-based peptide/protein evidence in fish. This draft proteome map of Labeo rohita would advance basic and applied research in aquaculture to meet the most critical challenge of providing food and nutritional security to an increasing world population.

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“…The function of zinc inseminal plasma controls the "cell membrane and nuclear chromatin of spermatozoa", and antibacterial function andprotectsdegenerative changes in the testis (4)(5)(6). with an additional role in regulatory of capacitation and acrosome reaction process (7). It is questionable how zinc relates to the parameters of semen quality that are usually determined.…”

Section: Introductionmentioning

confidence: 99%

Association of Zinc Concentration in Seminal Plasma and Blood with Several Parameters of Semen in Infertile Males

Saeedullah¹,

Shah²,

Hakim³

et al. 2022

PJMHS

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Objective: To evaluate the concentration of zinc inthe blood and seminal plasma with semen parameters between fertile and infertile males Methodology: This prospective study was carried out in the Department of Biochemistry, Hayatabad medical complex, Peshawar. Fifty (50)infertile males, not receiving any treatment and had regularintercourse for 1 yearaged 25-45 years were selected from HMC. They were divided into 35oligospermia and 15 azoospermic. 35 fertile males were nominatedamongthe overallresidents and were taken as the control group after their semen analysis was taken as normozoospermic. According to WHO criteria semen is analyzed. With the help of 5br Serum and seminal plasma zinc were estimated. Result: There was a significant difference found in serum and seminal levels of zinc in normozoospermic, oligospermia (p less than 0.05), and azoospermic (p-value less than 0.05) individuals. The correlation of seminal plasma zinc with sperm counts showed a positive association with the count of sperm and a negative association with motility of sperm in normozoospermic/oligospermia and a negative association of concentration with pH, and volume, WBC in all groups. No association was found with the morphology of sperm. Conclusion: It has been concluded that zinc interfered with fertility by having a considerable impact on many semen parameters. It suggests that measuring seminal plasma zinc could be useful for diagnosing and treating male infertility.

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Biochemical Assessments of Seminal Plasma Zinc, Testis-Expressed Sequence 101 and Free Amino Acids and Their Correlations with Reproductive Hormones in Male Infertility

Cited by 12 publications

References 50 publications

Metabolomics of Human Semen: A Review of Different Analytical Methods to Unravel Biomarkers for Male Fertility Disorders

Metabolomics of Human Semen: A Review of Different Analytical Methods to Unravel Biomarkers for Male Fertility Disorders

The Proteome Map and PeptideAtlas of a widely cultivated tropical water fish Labeo rohita: A resource for the Aquaculture Community

Association of Zinc Concentration in Seminal Plasma and Blood with Several Parameters of Semen in Infertile Males

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