Biochemical aspects of seeds from Cannabis sativa L. plants grown in a mountain environment

Abstract

Cannabis sativa L. (hemp) is a versatile plant which can adapt to various environmental conditions. Hempseeds provide high quality lipids, mainly represented by polyunsaturated acids, and highly digestible proteins rich of essential aminoacids. Hempseed composition can vary according to plant genotype, but other factors such as agronomic and climatic conditions can affect the presence of nutraceutic compounds. In this research, seeds from two cultivars of C. sativa (Futura 75 and Finola) grown in a mountain environment of the Italian Alps were analyzed. The main purpose of this study was to investigate changes in the protein profile of seeds obtained from such environments, using two methods (sequential and total proteins) for protein extraction and two analytical approaches SDS-PAGE and 2D-gel electrophoresis, followed by protein identification by mass spectrometry. The fatty acids profile and carotenoids content were also analysed. Mountain environments mainly affected fatty acid and protein profiles of Finola seeds. These changes were not predictable by the sole comparison of certified seeds from Futura 75 and Finola cultivars. The fatty acid profile confirmed a high PUFA content in both cultivars from mountain area, while protein analysis revealed a decrease in the protein content of Finola seeds from the experimental fields.

Phenolic profiles of non‐industrial hemp (Cannabis sativa L.) seed varieties collected from four different Moroccan regions

“…Cannabis can be classified into two main chemotypes based on its THC/CBD ratio. By consensus, industrial cannabis has a low THC content (< 0.2%) with a THC/CBD ratio lower than 1, while non-industrial cannabis, also called drug-type, has high THC values (> 0.2%) with a ratio greater than 1 (Cattaneo et al, 2021).…”

Metabolomics Analysis Revealed the Characteristic Metabolites of Hemp Seeds Varieties and Metabolites Responsible for Antioxidant Properties

“…Metabolome analysis showed that 236 metabolites were differentially accumulated in two local hemp seed breeding varieties with different dimensions in India ( Rashid et al, 2021 ). Being planted in the mountain environment greatly affects the fatty acids profile and carotenoids content of hemp seeds ( Cattaneo et al, 2021 ). Proteomic signatures also indicated that there are differences between two hemp seed varieties ( Park et al, 2012 ; Aiello et al, 2016 ).…”

A review on the techno‐functional, biological, and health‐promoting properties of hempseed‐derived proteins and peptides

“…Albumin, majorly edestin, makes up a higher proportion of the protein content of hempseed (60%–80%) while the rest of the protein content is globulin (House et al, 2010). Similarly, hempseed is rich in polyunsaturated fatty acids (Cattaneo et al, 2021; Razmaitė et al, 2021), and consumption of a hempseed‐supplemented diet was shown to increase the breast milk quality of dairy goat (Rapetti et al, 2021). Similarly, hempseed oil protected Drosophila melanogaster larvae from experimentally‐induced oxidative stress (Vitorović et al, 2021) and improved the nutritional and immune profile of offspring of piglets fed hempseed oil‐supplemented diet (Vodolazska & Lauridsen, 2020).…”