Biochemical and transcriptomic analyses of two bovine skeletal muscles in Charolais bulls divergently selected for muscle growth

Sudre, K.; Cassar-Malek, Isabelle; Listrat, Anne; Ueda, Yuichi; Leroux, Christine; Jurie, Catherine; Auffray, Charles; Renand, Gilles; Martin, Patrice; Hocquette, Jean-François

doi:10.1016/j.meatsci.2005.01.012

Cited by 43 publications

(44 citation statements)

References 39 publications

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“…The de novo synthesis of fat in intramuscular adipocytes seems to occur mainly from glucose and less from acetate, as in bovine adipocytes from other fatty tissues (reviewed by Smith et al, 2009). Indeed, a higher level of GLUT4 expression and higher activities of metabolic enzymes involved in the conversion of glucose into long chain fatty acids (namely PFK and ATP-citrate lyase) were detected in intramuscular adipose tissue compared to subcutaneous fat in these species (Hocquette et al, 2005).…”

Section: Regulation Of Metabolism By Nutrition In Veal Calvesmentioning

confidence: 95%

“…Indeed, the comparison of two lines of Charolais young bulls obtained by divergent selection on growth rate and feed efficiency demonstrated that an increased lean-to-fat ratio was associated with greater muscle glycolytic metabolism, lower intramuscular fat content (Renand et al, 1994), a greater number of fibres, a higher proportion of fast glycolytic fibres and a lower proportion of slow fibres (Duris et al, 1999). Both biochemical and transcriptomic results indicate that selection on muscle growth potential is associated with reduced slow-oxidative muscle characteristics (Sudre et al, 2005). Furthermore, the Blonde d'Aquitaine breed, in which neither deletion nor mutation in the myostatin gene has yet been identified (Grobet et al, 1998), shows similar muscle characteristics to those of DM cattle (Listrat et al, 2001).…”

Section: Regulation Of Metabolism By Nutrition In Veal Calvesmentioning

confidence: 99%

“…Therefore, in some countries, especially in Europe, genetic selection in beef breeds has been directed in favour of muscle development in order to produce lean carcasses with the ultimate objective of increasing the production of muscle quantitatively at the expense of fat. Selective breeding in cattle has effectively addressed increasing muscle growth rates in beef cattle and this may have modified muscle characteristics involved in meat quality (Sudre et al, 2005;Bernard et al, 2009). In other countries, such as Australia, USA and especially Japan, the production of fat animals is favoured to ensure the production of marbled beef (which is beef rich in intramuscular fat), which has a high economic value.…”

Section: Introductionmentioning

confidence: 99%

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Endocrine and metabolic regulation of muscle growth and body composition in cattle

Hocquette

2010

Animal

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Muscle metabolism (in interaction with other organs and tissues, including adipose tissue) plays an important role in the control of growth and body composition. Muscle ontogenesis has been described in different genotypes of cattle for myofibres, connective tissue and intramuscular depots. The ontogenesis or the action of putatively important factors controlling muscle development (IGF-II expression, IGF receptors, growth hormone (GH) receptor, myostatin, basic fibroblast growth factor, transforming growth factor-b1, insulin and thyroid hormones) has also been studied on bovine foetal muscle samples and satellite cells. The glucose/ insulin axis has been specifically studied in both the bovine adipose tissue and heart. Clearly, cattle, like sheep, are mature species at birth based on their muscle characteristics compared to other mammalian or farm animal species. The different myoblast generations have been well characterised in cattle, including the second generation which is liable to be affected by foetal undernutrition at least in sheep. Interesting genotypes, for example, double-muscled genotype, have been characterised by an altered metabolic and endocrine status associated with a reduced fat mass, specific muscle traits and different foetal characteristics. Finally, the recent development of genomics in cattle has allowed the identification of novel genes controlling muscle development during foetal and postnatal life. Generally, a high muscle growth potential is associated with a reduced fat mass and a switch of muscle fibres towards the glycolytic type. The possibility and the practical consequences of manipulating muscle growth and, hence, body composition by nutritional and hormonal factors are discussed for bovines based on our current biological knowledge.

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Section: Regulation Of Metabolism By Nutrition In Veal Calvesmentioning

confidence: 95%

Section: Regulation Of Metabolism By Nutrition In Veal Calvesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Endocrine and metabolic regulation of muscle growth and body composition in cattle

Hocquette

2010

Animal

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“…Other studies focussed on fetal muscle development (Sudre et al 2003;Lehnert et al 2007), muscle growth potential (Sudre et al 2005;Cassar-Malek et al 2007) and effects of nutritional changes , which all influence the composition of muscle tissue. Intramuscular fat development was also examined Lee et al 2007) since it influences marbling and thus juiciness and flavour.…”

Section: Application To Meat Qualitymentioning

confidence: 99%

Application of gene expression studies in livestock production systems: a European perspective

Cassar-Malek

Picard

Bernard

et al. 2008

Aust. J. Exp. Agric.

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Abstract. In the context of sustainable agriculture and animal husbandry, understanding animal physiology remains a major challenge in the breeding and production of livestock, especially to develop animal farming systems that respond to the new and diversified consumer demand. Physiological processes depend on the expression of many genes acting in concert. Considerable effort has been expended in recent years on examining the mechanisms controlling gene expression and their regulation by biological and external factors (e.g. genetic determinants, nutritional factors, and animal management). Two main strategies have been developed to identify important genes. The first one has focussed on the expression of candidate genes for key physiological pathways at the level of both the transcripts and proteins. An original strategy has emerged with the advent of genomics that addresses the same issues through the examination of the molecular signatures of all genes and proteins using high-throughput techniques (e.g. transcriptomics and proteomics). In this review, the application of the gene expression studies in livestock production systems is discussed. Some practical examples of genomics applied to livestock production systems (e.g. to optimise animal nutrition, meat quality or animal management) are presented, and their outcomes are considered. In the future, integration of the knowledge gained from these studies will finally result in optimising livestock production systems through detection of desirable animals and their integration into accurate breeding programs or innovative management systems.

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“…Producers in Australia have been successfully using a range of selection methods to improve retail beef yield, including BREEDPLAN estimated breeding values for eye muscle area and meat yield percentage, gene markers for the non-functional myostatin -E-mail: p.mcgilchrist@murdoch.edu.au gene (O'Rourke et al, 2009) and visual muscling selection techniques. There is good evidence that these selection techniques have increased muscling, reduced fat proportions in the carcass (Perry et al, 1993b;O'Rourke et al, 2009) and reduced slow-oxidative muscle characteristics (Sudre et al, 2005), however there has been minimal research to show the impact of this selection on carbohydrate metabolism in the muscle and lipid metabolism in adipose tissue.…”

Section: Introductionmentioning

confidence: 99%

Beef cattle selected for increased muscularity have a reduced muscle response and increased adipose tissue response to adrenaline

McGilchrist

Pethick

Bonny

et al. 2011

Animal

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The aim of this experiment was to evaluate the impact of selection for greater muscling on the adrenaline responsiveness of muscle, adipose and liver tissue, as reflected by changes in plasma levels of the intermediary metabolites lactate, non-esterified fatty acids (NEFA) and glucose. This study used 18-month-old steers from an Angus herd visually assessed and selected for divergence in muscling for over 15 years. Ten low muscled (Low), 11 high muscled (High) and 3 high muscled heterozygotes for myostatin mutation (High Het ) steers were challenged with adrenaline doses ranging between 0.2 to 3.0 mg/kg live weight. For each challenge, 16 blood samples were taken between 230 and 130 min relative to adrenaline administration. Plasma was analysed for NEFA, lactate and glucose concentration and area under curve (AUC) over time was calculated to reflect the tissue responses to adrenaline. Sixteen basal plasma samples from each animal were also assayed for growth hormone. Muscle glycogen and lactate concentration were analysed from four muscle biopsies taken from the semimembranosus, semitendinosus and longissimus thoracis et lumborum of each animal at 14, 90 and 150 days on an ad libitum grain-based diet and at slaughter on day 157. In response to the adrenaline challenges, the High steers had 30% lower lactate AUC than the Low steers at challenges greater than 2 mg/kg live weight, indicating lower muscle responsiveness at the highest adrenaline doses. Aligning with this decrease in muscle response in the High animals were the muscle glycogen concentrations which were 6.1% higher in the High steers. These results suggest that selection for muscling could reduce the incidence of dark, firm, dry meat that is caused by low levels of glycogen at slaughter. At all levels of adrenaline challenge, the High steers had at least 30% greater NEFA AUC, indicating that their adipose tissue was more responsive to adrenaline, resulting in greater lipolysis. In agreement with this response, the High steers had a higher plasma growth hormone concentration, which is likely to have contributed to the increased lipolysis evident in these animals in response to adrenaline. This difference in lipolysis may in part explain the reduced fatness of muscular cattle. There was no effect of selection for muscling on liver responsiveness to adrenaline.

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Biochemical and transcriptomic analyses of two bovine skeletal muscles in Charolais bulls divergently selected for muscle growth

Cited by 43 publications

References 39 publications

Endocrine and metabolic regulation of muscle growth and body composition in cattle

Endocrine and metabolic regulation of muscle growth and body composition in cattle

Application of gene expression studies in livestock production systems: a European perspective

Beef cattle selected for increased muscularity have a reduced muscle response and increased adipose tissue response to adrenaline

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