Biochemical and transcriptomic analyses of drought stress responses of LY1306 tobacco strain

Yang, Huijuan; Zhao, Li; Zhao, Shimin; Wang, Jing; Shi, Hongzhi

doi:10.1038/s41598-017-17045-2

Cited by 32 publications

(27 citation statements)

References 33 publications

(30 reference statements)

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“…For example, the transcription of CAT3 could be induced by drought and overexpression lines were insensitive to drought stress (Zou et al , 2015). In tobacco, biochemical and transcriptomic analyses showed that CAT transcript level and catalase enzyme activity were up‐regulated by drought stress treatment (Yang et al , 2017; Yin et al , 2015). The results we obtained are consistent with the view that NtCAT expression was induced by drought, and overexpression lines had high survival rates under drought stress conditions (Figure 1), suggesting that NtCAT acts as a positive regulator of drought tolerance.…”

Section: Discussionmentioning

confidence: 99%

The AP2 transcription factor NtERF172 confers drought resistance by modifying NtCAT

Zhao

Liú

et al. 2020

Plant Biotechnology Journal

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Drought stress often limits plant growth and global crop yields. Catalase (CAT)-mediated hydrogen peroxide (H 2 O 2) scavenging plays an important role in the adaptation of plant stress responses, but the transcriptional regulation of the CAT gene in response to drought stress is not well understood. Here, we isolated an APETALA2/ETHYLENE-RESPONSIVE FACTOR (AP2/ERF) domain-containing transcription factor (TF), NtERF172, which was strongly induced by drought, abscisic acid (ABA) and H 2 O 2 , from tobacco (Nicotiana tabacum) by yeast one-hybrid screening. NtERF172 localized to the nucleus and acted as a transcriptional activator. Chromatin immunoprecipitation, yeast one-hybrid assays, electrophoretic mobility shift assays and transient expression analysis assays showed that NtERF172 directly bound to the promoter region of the NtCAT gene and positively regulated its expression. Transgenic plants overexpressing NtERF172 displayed enhanced tolerance to drought stress, whereas suppression of NtERF172 decreased drought tolerance. Under drought stress conditions, the NtERF172-overexpressed lines showed higher catalase activity and lower accumulation of H 2 O 2 compared with wild-type (WT) plants, while the NtERF172-silenced plants showed the inverse correlation. Exogenous application of amino-1,2,4-triazole (3-AT), an irreversible CAT inhibitor, to the NtERF172-overexpression lines showed decreased catalase activity and drought tolerance, and increased levels of cellular H 2 O 2. Knockdown of NtCAT in the NtERF172-overexpression lines displayed a more drought stresssensitive phenotype than NtERF172-overexpression lines. We propose that NtERF172 acts as a positive factor in drought stress tolerance, at least in part through the regulation of CATmediated H 2 O 2 homeostasis.

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Section: Discussionmentioning

confidence: 99%

The AP2 transcription factor NtERF172 confers drought resistance by modifying NtCAT

Zhao

Liú

et al. 2020

Plant Biotechnology Journal

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“…PYL8 positively regulates ABA signaling during germination and abiotic stress responses. Furthermore, data showed that PYL8 plays essential roles in response to dark-induced senescence [102] and in drought stress [103]. An SNP variation was detected on a Cysteine protease response to dehydration (RD19A-like), which is a responsive gene to salt stress.…”

Section: Discussionmentioning

confidence: 99%

EST–SNP Study of Olea europaea L. Uncovers Functional Polymorphisms between Cultivated and Wild Olives

Mariotti

Belaj

Rosa

et al. 2020

Genes

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Background: The species Olea europaea includes cultivated varieties (subsp. europaea var. europaea), wild plants (subsp. europaea var. sylvestris), and five other subspecies spread over almost all continents. Single nucleotide polymorphisms in the expressed sequence tag able to underline intra-species differentiation are not yet identified, beyond a few plastidial markers. Methods: In the present work, more than 1000 transcript-specific SNP markers obtained by the genotyping of 260 individuals were studied. These genotypes included cultivated, oleasters, and samples of subspecies guanchica, and were analyzed in silico, in order to identify polymorphisms on key genes distinguishing different Olea europaea forms. Results: Phylogeny inference and principal coordinate analysis allowed to detect two distinct clusters, clearly separating wilds and guanchica samples from cultivated olives, meanwhile the structure analysis made possible to differentiate these three groups. Sequences carrying the polymorphisms that distinguished wild and cultivated olives were analyzed and annotated, allowing to identify 124 candidate genes that have a functional role in flower development, stress response, or involvement in important metabolic pathways. Signatures of selection that occurred during olive domestication, were detected and reported. Conclusion: This deep EST–SNP analysis provided important information on the genetic and genomic diversity of the olive complex, opening new opportunities to detect gene polymorphisms with potential functional and evolutionary roles, and to apply them in genomics-assisted breeding, highlighting the importance of olive germplasm conservation.

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“…The T1 generations were said to be the transgenic tobacco lines that contained a single insertion, whose seeds were grown in the same conditions as described earlier. After three weeks, the seedlings were transferred to a hydroponic system and two weeks later, the seedlings of the transgenic tobacco were exposed to 100 mM NaCl [ 34 ], 15% PEG-6000 ( m / v ) [ 35 ] and 100 mM H 2 O 2 [ 36 ] in half strength Hoagland’s nutrient solution (24 h) for simulating salt, drought and oxidative stress, respectively. For cold stress, the seedlings were cultivated in half strength Hoagland’s solution at 4 °C [ 37 ].…”

Section: Methodsmentioning

confidence: 99%

Cloning and Functional Analysis of Phosphoethanolamine Methyltransferase Promoter from Maize (Zea mays L.)

Niu

Gou

Han

et al. 2018

IJMS

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Betaine, a non-toxic osmoprotectant, is believed to accumulate considerably in plants under stress conditions to maintain the osmotic pressure and promote a variety of processes involved in growth and development. Phosphoethanolamine N-methyltransferase (PEAMT), a key enzyme for betaine synthesis, is reported to be regulated by its upstream promoter. In the present investigation, by using the transgenic approach, a 1048 bp long promoter region of ZmPEAMT gene from Zea mays was cloned and functionally characterized in tobacco. Computational analysis affirmed the existence of abiotic stress responsive cis-elements like ABRE, MYC, HST, LST etc., as well as pathogen, wound and phytohormone responsive motifs. For transformation in tobacco, four 5′-deletion constructs of 826 bp (P2), 642 bp (P3), 428 bp (P4) and 245 bp (P5) were constructed from the 1048 bp (P1) promoter fragment. The transgenic plants generated through a single event exhibited a promising expression of GUS reporter protein in the leaf tissues of treated with salt, drought, oxidative and cold stress as well as control plants. The GUS expression level progressively reduced from P1 to P5 in the leaf tissues, whereas a maximal expression was observed with the P3 construct in the leaves of control plants. The expression of GUS was noted to be higher in the leaves of osmotically- or salt-treated transgenic plants than that in the untreated (control) plants. An effective expression of GUS in the transgenic plants manifests that this promoter can be employed for both stress-inducible and constitutive expression of gene(s). Due to this characteristic, this potential promoter can be effectively used for genetic engineering of several crops.

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Biochemical and transcriptomic analyses of drought stress responses of LY1306 tobacco strain

Cited by 32 publications

References 33 publications

The AP2 transcription factor NtERF172 confers drought resistance by modifying NtCAT

The AP2 transcription factor NtERF172 confers drought resistance by modifying NtCAT

EST–SNP Study of Olea europaea L. Uncovers Functional Polymorphisms between Cultivated and Wild Olives

Cloning and Functional Analysis of Phosphoethanolamine Methyltransferase Promoter from Maize (Zea mays L.)

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