Biochemical and serological identification of major histocompatibility complex antigens in outbred chickens

Pinard, M.H.; Hepkema, Bouke

doi:10.1016/0165-2427(93)90071-b

Cited by 4 publications

(1 citation statement)

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“…The general appearance of only a few haplotypes (B2, B15 and B21) may re£ect the importance of these haplotypes. The B2 and B21 haplotypes derived from White Leghorns Witter, 1992, 1994a,b) and B21-like haplotypes in Brown layers (Hepkema et al, 1991;Pinard and Hepkema, 1993;Parmentier et al, 2002) are generally associated with enhanced immune competence and enhanced disease resistance. The B15 of White Leghorns is often related to enhanced disease susceptibility Witter, 1992, 1993).…”

Section: Discussionmentioning

confidence: 99%

Serological Screening for MHC (B)-Polymorphism in Indigenous Chickens

Baelmans

Parmentier

Nieuwland

et al. 2005

Tropical Animal Health and Production

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As part of a series of studies to characterize innate and speci¢c immune responses of indigenous chicken lines, birds from Bolivia and India were screened serologically for MHC class IV (BG) polymorphism by direct haemagglutination using haplotype-speci¢c antisera (B2, B4, B12, B13, B14, B15, B19, B21). The sample consisted of 95 Bolivian indigenous chickens and 119 hens from the four most common North Indian`back-yard' chicken lines: Yellow Aseel (AP), Kadaknath (KN), frizzled typed (Ff^) and naked neck (NN). Of all chickens tested, the majority were haplotyped as B2, B15, B19 and B21. Of the Bolivian chickens, 89.5% could be haplotyped: 54.9% were homozygous (including 43.3% B15), and 34.6% were heterozygous (including 15.7% B15). B2-like haplotypes were not found among the Bolivian hens, and only 3.2% of these birds showed homozygous B21-like proteins. Of the Indian hens, MHC (BG)-like proteins could be detected in 60.0% of the AP birds, 6.7% of the KN birds; 40.0% of the Ff^birds; and 10.3% of the NN birds. In these lines, a total of 40.1% (AP), 6.7% (KN), 30.1% (Ff^) and 10.3% (NN) were homozygous for the B-haplotype. Only in the AP line (19.9%), and the Ff^line (9.9%) were heterozygous B-haplotypes types found. The B2 haplotype was found in all Indian chicken lines. Most Indian birds have completely unknown haplotypes, indicating a potentially interesting genetic pool. Subgrouping the Bolivian and Indian indigenous hens into monomorphic BG populations revealed individual di¡erences based on the B-types.

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Section: Discussionmentioning

confidence: 99%

Serological Screening for MHC (B)-Polymorphism in Indigenous Chickens

Baelmans

Parmentier

Nieuwland

et al. 2005

Tropical Animal Health and Production

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Diversity and evolution of the highly polymorphic tandem repeat LEI0258 in the chicken MHC-B region

et al. 2013

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The chicken major histocompatibility complex (MHC) is located on the microchromosome 16 and is described as the most variable region in the genome. The genes of the MHC play a central role in the immune system. Particularly, genes encoding proteins involved in the antigen presentation to T cells. Therefore, describing the genetic polymorphism of this region is crucial in understanding host-pathogen interactions. The tandem repeat LEI0258 is located within the core area of the B region of the chicken MHC (MHC-B region) and its genotypes correlate with serology. This marker was used to provide a picture of the worldwide diversity of the chicken MHC-B region and to categorize chicken MHC haplotypes. More than 1,600 animals from 80 different populations or lines of chickens from Africa, Asia, and Europe, including wild fowl species, were genotyped at the LEI0258 locus. Fifty novel alleles were described after sequencing. The resulting 79 alleles were classified into 12 clusters, based on the SNPs and indels found within the sequences flanking the repeats. Furthermore, hypotheses were formulated on the evolutionary dynamics of the region. This study constitutes the largest variability report for the chicken MHC and establishes a framework for future diversity or association studies.

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