Biochemical and serological identification of strains of Haemophilus pleuropneumoniae

Humoral antibody response and protective immunity in swine following immunization with the 104-kilodalton hemolysin of Actinobacillus pleuropneumoniae

“…The percentage of the total lung volume with pleuropneumonia was estimated. Samples of lungs and tonsils were cultured on chocolate blood agar supplemented with 0.01% NAD and incubated in CO2 at 37°C for 24 h. Typical colonies were subcultured and confirmed as A. pleuropneumoniae as described elsewhere (16). Serum samples were taken from each pig before the first subcutaneous injection (preimmune), 1 week after the last injection but before challenge (prechallenge), and after challenge (postchallenge).…”

A Comparative Study of the Preventive Use of Tilmicosin Phosphate (Pulmotil premix®) andMycoplasma hyopneumoniaeVaccination in a Pig Herd with Chronic Respiratory Disease

“…Fifteen blood samples taken at slaughter from each group were tested for the presence of antibodies against porcine reproductive and respiratory syndrome virus (PRRSV). A standard haemagglutination‐inhibition test was used to detect antibodies against the influenza viruses (Palmer et al., 1975), a complement fixation test was used to detect antibodies against the App serovars (Lombin et al., 1985) and the Herd Check ® PRRS ELISA (Idexx Laboratories, Westbrook, ME) was used to detect PRRSV antibodies. HI titres ≥ 4 and ≥ 20 were considered positive for H1N1 and H3N2 viruses, respectively.…”

Actinobacillus pleuropneumoniaesurface polysaccharides: their role in diagnosis and immunogenicity

“…Several techniques have been described for the serological characterization of A. pleuropneumoniae. Tests developed to determine the serotypes depend largely on surface polysaccharides and include tube agglutination (Gunnarsson et al, 1977), immunofluorescence (Nicolet, 1971;Rosendal et al, 1981b), immunodiffusion (Nicolet, 1971), slide agglutination (Mittal et al, 1982), the 2-mercaptoethanol tube agglutination test (Mittal et al, 1984(Mittal et al, , 1987aMittal and Bourdon, 1991), ring precipitation (Mittal et al, 1982(Mittal et al, , 1987a, indirect hemagglutination (Mittal et al, 1983a), coagglutination (Mittal et al, 1983b), counter-immunoelectrophoresis (Piffer et al, 1986;Mittal et al, 1993b), complement fixation Lombin et al, 1985), paper chromatography (Utrera et al 1988) slide precipitation (Hommez et al, 1990).…”