Biochemical and Scanning Electron Microscopic Properties of Mitochondria Isolated From Rat Cardiac Muscle

Abstract

Cardiac muscle mitochondrial fraction was prepared from rat heart homogenate, according to the procedure of differential centrifugation fol lowing nagarse digestion. The biochemical intactness of isolated mitochon dria was identified by determining the oxygen consumption of respiratory chain. The field emission scanning microscope examination of the isolated mitochondria indicated that mitochondria maintained their structural in tegrity throughout the isolation procedure. The mitochondrial fraction treated with nagarse in the process of pre paration was identified to be intact and useful for the study of cardiac muscle mitochondria.

Experimental study on the effect of CoQ-10 administration to isoproterenol-induced cardiomyopathy of rats.

“…In contrast with IPA experiment, administration of adriamycin caused a marked deficiency of CoQ-10 in rat heart mitochondria, and the exogenous CoQ-10 restored the CoQ-10 content in the heart mitochondria of adriamycin-treated rats to the normal level (Toyama, 1979;Ogura et al, 1979). The discrepant evidence of CoQ-10 in mitochondria is not able to explain, but the pathological mechanism of induced cardiomyopathy may be different.…”

“…Yamagami et al (1967) reported that the state 4 respiration increased in canine with infarcted heart. Sayanagi (1977), Yamauchi (1978) and Nagata (1979 in our laboratory have reported the relation of the mitochondrial respiratory disorders to the lipid peroxide formed in mitochondria exposed to ultraviolet light. The antioxidant compounds such as vitamin B2 and CoQ-10 was found to prevent the increase of lipid peroxide and restore the respiratory disorders of mitochondria.…”

“…The increasing molecular changes in membrane may lead to biochemical and morphological damages to the cells (Goldstein et al, 1967) and organs (Thomas et al, 1967). There are many reports concerning deleterious effects of lipid peroxide in ethanol-induced liver injury (Ci Luzio, 1967), in oxygen toxicity (Haugaard, 1968) and in adriamycin-induced cardiotoxicity (Toyama, 1979). The lipid peroxidation is known to involve free radical formation in the peroxidation process.…”

“…Co. Ltd, U.S.A.) was used to determine the oxygen utilization of mitochondria polarographically in reaction mixture (Ogura et al 1979). Three ml of reaction medium consisting of 250mM mannitol, 10mM KH2PO4, 10mM KCI, 2 mM MgCl2 and 0.2mM EDTA in 10 mM Tris-HCl buffer (pH 7.4).…”

Assay for flavins in rat heart mitochondria by high pressure liquid chromatography.

“…Typical values obtained from a polarographic assay of mitochondrial respiratory responses are shown in Table 2. The morphological properties of the mitochondria prepared by this procedure were examined by transmission electron microscopy with the method of Shimada et al (Ogura et al 1979). As shown in Fig.…”