Biochemical and rheological characterization of a protease from fruits of Withania coagulans with a milk-clotting activity

Beigomi, Maryam; Mohammadifar, Mohammad Amin; Hashemi, Maryam; Rohani, M Ghods; Senthil, Kalaiselvi; Valizadeh, Mohharam

doi:10.1007/s10068-014-0247-5

Cited by 20 publications

(19 citation statements)

References 25 publications

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“…; Beigomi et al . ). Rennet application for cheese production is well documented dating back to 6000 B. C. (Fox and McSweeney ).…”

Section: Introductionmentioning

confidence: 97%

“…Cheese manufacturing is one of the most significant methods of preserving the major milk constituents (Khan and Masud 2013). The most important step during cheesemaking is the milk coagulation, achieved through the addition of calf rennet and more recently with microbial rennet (usually Renault et al 2000;Beigomi et al 2014). Rennet application for cheese production is well documented dating back to 6000 B. C. (Fox and McSweeney 1999).…”

Section: Introductionmentioning

confidence: 99%

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Effect of different storage conditions on coagulating properties and cheese quality of Withania coagulans extract

Qazalbash

Masud

Sammi

et al. 2017

Int J of Dairy Tech

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The effects of different storage conditions on the coagulating properties of extracts of Withania coagulans berries were investigated in terms of coagulation time, pH and quality attributes of cheese prepared from buffalo milk. The extracts were stored under different conditions, viz. room temperature (27 ± 3 °C), refrigerated storage (4 °C), frozen storage (−20 °C) and lyophilisation. The milk‐coagulating activity of the plant extracts was measured on a fortnightly basis for a period of 5 months. There was a nonsignificant change in pH and the milk coagulating properties of the lyophilised extract. Physiochemical analysis revealed that cheese prepared with lyophilised extract retained the highest content of ash (2.3%), fat (23.8%), total solids (48.7%) and crude protein (22.7%), and resulted in the highest cheese yield (17.6%) compared to other tested treatments. Thus, it is concluded that lyophilisation has good potential for storage of vegetable extract coagulants.

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“…; Beigomi et al . ). Rennet application for cheese production is well documented dating back to 6000 B. C. (Fox and McSweeney ).…”

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Effect of different storage conditions on coagulating properties and cheese quality of Withania coagulans extract

Qazalbash

Masud

Sammi

et al. 2017

Int J of Dairy Tech

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“…[18,19] However, the MCA is mostly attributed to the fruit pulp and husk and is due to the presence of an enzyme in its fruits which called plant rennin. [20,21] This work was undertaken to elucidate the properties of W. coagulans fruit extracts in order to use it in cheese making as an alternative to rennet. Also, isolation, partial purification and characterization of a milk-clotting protease from this traditional cheese maker plant were the other aims which addressed in this study for the first time.…”

Section: Introductionmentioning

confidence: 99%

Clotting characteristics of milk by Withania coagulans: Proteomic and biochemical study

Kazemipour

Salehi

Valizadeh

et al. 2016

International Journal of Food Properties

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Extract of Withania coagulans fruit was evaluated for milk clotting activity. The highest enzyme activities were detected at pH 4 and 65°C. A 50 mM CaCl 2 was considered sufficient to decrease the milk clotting activity to an effective level. Rennet coagulation time was steadily increased with increasing NaCl and decreasing enzyme concentrations. The enzymatic activity of extract was completely inhibited by pepstatin-A, an aspartic protease inhibitor. Proteomic and zymographic analysis discovered an aspartic protease with Mw of about 35 KDa and pI of 5.91. Our data demonstrated that use of this plant in traditional cheese making has the scientific bases due to existence of an effective protease.

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“…Many natural products stemming from plants have been shown to restrain cell proliferation and induce apoptosis, as well as promoting the management of BPH (11, 12). Withania coagulans (WC) is a traditional medicine from the Solanaceae family that is used for the treatment of various health problems and diseases in South Asia and the southeast regions of Iran (13). WC is well known for its antioxidant, anti-inflammatory, antiproliferative, and anti-cancerous properties (14-16).…”

Section: Introductionmentioning

confidence: 99%

Withania coagulans Extract Induces Cell Apoptosis and Inhibits COX-2 Expression in a Rat Model of Benign Prostatic Hyperplasia

2016

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BackgroundPhytotherapy is a popular treatment option in cases of benign prostatic hyperplasia (BPH), with many different herbal products being used for the treatment of this condition. Withania coagulans (WC) is an herbal medicine that has shown anti-tumoral, anti-inflammatory, and antioxidant effects.ObjectivesThis study examined the effect of Withania coagulans extract (WCE) on prostatic cell apoptosis and cyclooxygenase-2 (COX-2) expression in cases of benign prostatic hyperplasia (BPH) in rats.MethodsForty Wistar rats were equally divided into five groups: control, sham, BPH, BPH + WCE, and BPH + CLX (celecoxib) as a positive control group. The induction of BPH was achieved via the subcutaneous injection of 3 mg/kg of testosterone propionate (TP) daily for 28 days. The animals received WCE, celecoxib, or distilled water by oral gavage accompanied by the TP injection. After four weeks, the prostate glands of the rats were weighed to measure the prostatic index (PI). The ventral lobes of the prostates were dissected and processed with paraffin blocks in order to study the number of mast cells. A TUNEL analysis was performed to evaluate the cell apoptosis, while the expression of COX-2 was examined using immunohistochemistry.ResultsBPH was obvious in the ventral lobe of the prostate, and the administration of WCE markedly decreased the PI and the number of mast cells (P < 0.001) in the BPH rats. Additionally, the WCE treatment induced prostatic cell apoptosis when compared to the BPH group. Furthermore, following the WCE treatment, the expression of COX-2 in the prostatic tissues was significantly decreased when compared to the BPH groups.ConclusionsAccording to the results of this study, WCE was effective in the treatment of BPH in rats. It may therefore have beneficial effects in the treatment of patients with BPH.

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Biochemical and rheological characterization of a protease from fruits of Withania coagulans with a milk-clotting activity

Cited by 20 publications

References 25 publications

Effect of different storage conditions on coagulating properties and cheese quality of Withania coagulans extract

Effect of different storage conditions on coagulating properties and cheese quality of Withania coagulans extract

Clotting characteristics of milk by Withania coagulans: Proteomic and biochemical study

Withania coagulans Extract Induces Cell Apoptosis and Inhibits COX-2 Expression in a Rat Model of Benign Prostatic Hyperplasia

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