Biochemical and morphological characterization of freshwater microalga Tetradesmus obliquus (Chlorophyta: Chlorophyceae)

Cesário, Cristiane do Carmo; Soares, Jimmy; Cossolin, Jamile Fernanda Silva; Almeida, Allan Victor Martins; Sierra, José Jovanny Bermudez; Leite, Maurício de Oliveira; Nunes, Maria Clara; Serrão, José Éduardo; Martins, Márcio Arêdes; Coimbra, Jane Sélia dos Reis

doi:10.1007/s00709-021-01712-3

Cited by 11 publications

(13 citation statements)

References 63 publications

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“…The cells of T. obliquus are rhombic, in coenobia of 2, 4, and 8 cells. The aggregated cells of T. obliquus are generally found as four-celled coenobia, with a length of 10-21 µm and a width of 3-9 µm [23,24]. The cellular morphology of strain ZYY1 was similar to that of T. obliquus.…”

Section: Microalgae Isolation Screening and Identificationmentioning

confidence: 90%

Sustainable Treatment of Swine Wastewater: Optimizing the Culture Conditions of Tetradesmus cf. obliquus to Improve Treatment Efficiency

Bai,

Qu,

Song

et al. 2024

Sustainability

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To enhance the sustainability of microalgae-based swine wastewater treatment, this study aims to address the challenges of low efficiency in treating raw swine wastewater, collection difficulties, and high energy consumption treatment processes. The microalgae with strong environmental tolerance were first screened from swine wastewater, and its cultivation conditions were optimized to examine the effect of microalgae treatment on swine wastewater under optimal cultivation conditions. Additionally, the flocculation efficiency and mechanism of microalgae were analyzed. The results showed that Tetradesmus cf. obliquus ZYY1 exhibited the most robust heterotrophic growth. In the BG11 medium supplemented with glucose, the growth rate of T. cf. obliquus ZYY1 under chemoheterotrophic conditions was superior to its growth under photoheterotrophic conditions, reaching its peak with an optimal glucose concentration of 15 g/L. The biomass concentration of T. cf. obliquus ZYY1 in raw wastewater was significantly higher than that in sterilized wastewater, which reached 1.65 ± 0.01 g/L on the 10th day of treatment, with removal efficiencies of -N, -P, and the chemical oxygen demand reached 71.36%, 96.09%, and 93.13%, respectively. After raw wastewater treatment, the flocculation efficiency of T. cf. obliquus ZYY1 reached 97.71 ± 5.81%. This was attributed to the bacteria present in the raw wastewater, which induced T. cf. obliquus ZYY1 to secrete aromatic proteins. This study emphasizes the potential of microalgae as a green technology for sustainable wastewater treatment, offering a practical pathway for environmental protection and resource conservation.

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Section: Microalgae Isolation Screening and Identificationmentioning

confidence: 90%

Sustainable Treatment of Swine Wastewater: Optimizing the Culture Conditions of Tetradesmus cf. obliquus to Improve Treatment Efficiency

Bai,

Qu,

Song

et al. 2024

Sustainability

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“…The microalgal biomass contained a high content of proteins (40.29 ± 0.24% w/w), which agrees with previous literature data for Tetradesmus spp. [13,20,21,37]. Thus, T. obliquus biomass is a strategic ingredient for the food industry since it is a potential alternative raw material for obtaining protein isolates and concentrates [20,21] that can be cultivated in large quantities for industrial feed applications in the food industry.…”

Section: Composition Of T Obliquus Biomassmentioning

confidence: 99%

“…Even the control sample showed the presence of cells with a reddish color, suggesting that some of the cells may have been damaged or ruptured. This behavior is probably due to cell disintegration and membrane permeabilization of microalgal species with rigid cell walls, such as T. obliquus [37], during freeze-drying [43]. The freeze-drying process causes some damage to the cell membrane, increasing its porosity; however, the cell wall is not ruptured [44][45][46].…”

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confidence: 99%

“…Concerning the wall structure, microalgae with cellulose carbohydrate-based cell walls typically show more resistance against ultrasound than cell walls mainly composed of hydroxyproline-rich glycoproteins [50]. According to Do Carmo Cesário et al [37], the T. obliquus cell wall is filled predominantly with fibrous material and has three well-defined layers, making breakdown even more challenging.…”

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confidence: 99%

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Evaluation of Cell Rupture Techniques for the Extraction of Proteins from the Microalgae Tetradesmus obliquus

da Silva,

Sibaja,

de Ramos Cizilio

et al. 2024

Phycology

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The high protein content of several microalgal species makes them attractive and unconventional candidates for use in the food and pharmaceutical industries. Due to the robust cell walls of microalgae, cell rupture is necessary to improve the extraction of intracellular proteins. Thus, choosing a suitable cell-breaking treatment before protein extraction is a vital downstream processing step. Additionally, it is necessary to use an effective technique for monitoring and measuring the impact of rupture treatments on microalgal cell walls. In our study, Tetradesmus obliquus cells were disrupted using three different mechanical rupture methods: high-pressure homogenization (HPH), ultrasound (US), and ball milling (BM). The ruptured biomass cells were counted, and soluble proteins were extracted and quantified. The cell-counting technique did not detect any differences between intact and damaged cells after BM treatment because the dye (erythrosine B) did not permeate the microalgal biomass accurately. The US treatment promoted the highest yield of total protein extraction (19.95%), while the highest yields in the HPH and BM treatments were 15.68% and 14.11%, respectively. Since the cell breakage method affects protein extraction from microalgal biomass, protein release can be used as a central indicator of the degree of cell disruption.

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“…Although T. obliquus has been well-studied for its great biotechnological potential and high content of proteins, carbohydrates, lipids, and bioactive compounds [57,58], there is only a little data on the content of its inorganic compounds. A very recent study on T. obliquus strain RDLR01 biosorption of heavy metals from a tannery effluent after 15 days of treatment demonstrated their reduction in the following descending line of percentage: Cr-99.1%, Co-98.2%, Ni-97.4%, Cd-97.4%, Pb-94.3%, Zn-98.3% and Cu-96.3% [59].…”

Section: Heavy (Hazardous) Metalsmentioning

confidence: 99%

Reclamation Potential of Onsite Wastewater Post-Treatment with Microalgae: Chemical Elements Perspective

et al. 2023

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Algae-based wastewater treatment is a promising technology with various applications for excess biomass such as biofertilizer production or valuable elements extraction. The benefits of the technology have been discussed for larger wastewater treatment plants (WWTPs), but the use of microalgae in decentralized wastewater treatment has been barely reported. The current study screens the possible resource recovery potential of onsite technology, which adds algae-based post-treatment to the conventional biological treatment of domestic wastewater. The effluent from the onsite sequencing batch reactor (SBR) of a household was further processed in laboratory conditions using an SBR technology with two local monocultures of algae—Klebsormidium nitens (Kützing) Lokhorst and Tetradesmus obliquus (Turpin) M. J. Wynne. The decant and the generated algal biomass were analyzed in terms of their element content. The post-treated effluent has a slightly better quality for irrigation purposes than the effluent of the onsite treatment facility—up to 1.6 times increased concentration for macro-elements and up to 1.9 times for micro elements. However, the generated algal biomass shows promising potential for re-use as a fertilizing agent since it contains valuable macro- and micro-elements and the heavy (hazardous) metal content is considerably lower than the limiting values in the current European and national legislations. The K. nitens strain may attract interest since it accumulates valuable metals such as chromium (36 mg/kgDS), nickel (83 mg/kgDS), and silver (0.7 mg/kgDS) that can be derived from the biomass and turn the technology to a circular one.

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Biochemical and morphological characterization of freshwater microalga Tetradesmus obliquus (Chlorophyta: Chlorophyceae)

Cited by 11 publications

References 63 publications

Sustainable Treatment of Swine Wastewater: Optimizing the Culture Conditions of Tetradesmus cf. obliquus to Improve Treatment Efficiency

Sustainable Treatment of Swine Wastewater: Optimizing the Culture Conditions of Tetradesmus cf. obliquus to Improve Treatment Efficiency

Evaluation of Cell Rupture Techniques for the Extraction of Proteins from the Microalgae Tetradesmus obliquus

Reclamation Potential of Onsite Wastewater Post-Treatment with Microalgae: Chemical Elements Perspective

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