Biochemical and morphological biomarker responses in the gills of a Neotropical fish exposed to a new flavonoid metal-insecticide

Sachi, Ivelise Teresa de Castro; Bonomo, Marina Marques; Sakuragui, Marise Margareth; Modena, Pamela Zaganin; Paulino, Marcelo Gustavo; Carlos, Rose Maria; Fernandes, João B.

doi:10.1016/j.ecoenv.2020.111459

Cited by 22 publications

(8 citation statements)

References 60 publications

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“…It is usually used in the evaluation of this toxicity due to its central role in the detoxification of both endogenous and xenobiotic compounds (Turhan et al, 2020), and play key roles in the antioxidant defence system (Abdalrahman et al, 2018). Several studies show that GST could be used as a biomarker in ecological risk assessment of pesticide contaminated environment (Costa et al, 2020; Sachi et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Chemical screening, insecticidal and reprotoxic activities of Tecoma stans ethanolic leaf extract against the vector mosquito Culex pipiens

Hafsi

Hamaidia

Soltani

2022

Physiological Entomology

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To select potential plant‐based insecticides, Tecoma stans (Bignoniaceae) leaf extract was screened for its larvicidal and delayed effects against a medically important mosquito species Culex pipiens L. (Diptera: Culicidae). First, gas chromatography–mass spectrometry (GC–MS) was conducted on T. stans extract, collected in ethanol for its chemical characterization and detection of active constituents. Second, insecticidal bioassays were made with several concentrations on earlier fourth instar larvae (L4) of Cx. pipiens for 24 h as recommended by WHO, in order to determine the lethality parameters of the tested extract. For that, two concentrations (LC30 and LC50) were applied on L4 for 24 h, and emerged adults were observed for their reproductive performance success like fecundity, percentage of hatching (fertility), body and gonads' volume. The biochemical composition of whole bodies of adults was investigated. Also, the specific activities of acetylcholinesterase (AChE) and glutathion S‐transferase (GST), biomarkers of neurotoxicity and detoxification, respectively, were also determined in L4 at different time intervals. Qualitative and quantitative phytochemical analysis (GC–MS) of the extract unveiled nine phytoconstituents with 2‐[4‐cyclohexylbutanoylamino]‐3‐chloro‐1,4‐naphthoquinone (36.24%) and 1,2‐Benzenedicarboxylic acid, mono(2‐ethylhexyl) ester (22.65%) as major components. The early L4 of Cx. pipiens were exposed to LC30 and LC50, and after 24 h, surviving individuals have been further reared until adult emergence. All over, T. stans leaves ethanolic extract impairs adults reproductive traits; it adversely affected gonad's size, fecundity and fertility, which negatively affected the survival of their offspring. Lipids contents were found to increase in both male and female adults. T. stans ethanolic extract increased GST activity and slightly inhibited AChE activity in L4. In conclusion, the ethanolic extract of T. stans could be a potential candidate used as a sustainable botanical insecticide for controlling mosquito.

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Section: Introductionmentioning

confidence: 99%

Chemical screening, insecticidal and reprotoxic activities of Tecoma stans ethanolic leaf extract against the vector mosquito Culex pipiens

Hafsi

Hamaidia

Soltani

2022

Physiological Entomology

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“…Hence modulation of GPx noted in our study suggests oxidative stress as the major mechanism involved in all toxic manifestation seen when fishes were exposed to EIE. Corroborating evidence exists from studies with other toxicants such as new flavonoid metalinsecticide, bisphenol-A, cadmium, chromium and copper on various fishes to support the findings that exposure to EIE altered antioxidant enzymes [4,37,38]. Taken together, oxidative stress seems to be the major physiological basis governing toxic exposure to electrochemical industrial effluent in the fish Cirrhinusmrigala.…”

Section: Discussionmentioning

confidence: 76%

Detection on Electroplating Effluent-Induced Cytopathological Alterations and DNA Damage in the Blood Samples of Cirrhinus mrigala (Hamilton, 1822)

Sudhasaravanan¹,

Binukumari²,

Rajeshkumar³

et al. 2021

austinjenvirontoxicol

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In the present study, fresh waterfish Cirrhinus mrigala has been used as a model organism to understand the mechanistic link between electroplating industrial effluent (EIE) and oxidative stress in aquatic ecosystems. To this end, the fishes were exposed to industrial effluent, in the tank water for 10, 20 and 30 days under controlled laboratory conditions. Various hematological parameters (Total RBC and WBC, Hemoglobin, MCV, MCH, PCV), biochemical metabolites (Glucose, protein, Cholesterol) and markers of genotoxicity (comet assay and micronuclear assay) were assessed. Fishes exposed for 30 days showed marked increase (p <0.001) in RBC with concomitant decrease in total WBC. There is significant (p <0.001) decline in indices of blood cells such as Hb, MCV, MCH and PCV. Results from comet assay showed significantly (p < 0.05) increased frequency genotoxicity in erythrocytes of as evident from observed increase in tail length in fishes exposed to EIE for 30 days. Overall, the results of this study, clearly demonstrated concentration dependent response of electroplating industrial effluent on freshwater fish Cirrhinus mrigala on haematological, biochemical and genotoxic markers. The results obtained herein suggests EIE as potential xenobiotics contributing towards ecotoxic and genotoxic effect in fishes.

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“…Gill is the main tissue of gas exchange and ion exchange in aquatic animals and is the first tissue to respond to air exposure stress. 28 In this study, we compared the gill transcriptome of mud crab S. paramamosain with air exposure for 8h and without air exposure. 20,128 DEGs were identified, among which 13,626 were up-regulated, and 6,502 were down-regulated.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome profiling of the gills to air exposure in mud crab Scylla paramamosain

Fan,

Guo,

Cheng

et al. 2024

Israeli Journal of Aquaculture - Bamidgeh

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The mud crab Scylla paramamosain is a valuable commercial mariculture crab worldwide. During the ebb tide or when transported to market, crabs suffer underlying air exposure stress. Gills tissue is the first tissue to cope with this stress. In this study, the transcriptome of S. paramamosain gills from the control group (CG) and experimental group (EG) were sequenced, assembled, and compared. A total of 7,425,620,293 bp and 6,741,616,977 bp clean data were found in EG and CG, respectively. A total of 38,507 unigenes (42.78%) were annotated successfully. 13,626 differentially expressed genes (DEGs) were up-regulated, and 6,502 DEGs were down-regulated. The DEGs related to immunity, apoptosis, metabolism, and ion exchange were detected. DEGs were enriched significantly into the KEGG pathways related to metabolism and immunity. These results proved that more material and energy were required, and immune defense was enhanced when the crab was under air exposure stress. The present study provides the first-gill transcriptomic analysis challenged with air exposure stress in S. paramamosain under air exposure stress, which will be useful to clarify the molecular mechanisms of air exposure adaptation.

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Biochemical and morphological biomarker responses in the gills of a Neotropical fish exposed to a new flavonoid metal-insecticide

Cited by 22 publications

References 60 publications

Chemical screening, insecticidal and reprotoxic activities of Tecoma stans ethanolic leaf extract against the vector mosquito Culex pipiens

Chemical screening, insecticidal and reprotoxic activities of Tecoma stans ethanolic leaf extract against the vector mosquito Culex pipiens

Detection on Electroplating Effluent-Induced Cytopathological Alterations and DNA Damage in the Blood Samples of Cirrhinus mrigala (Hamilton, 1822)

Transcriptome profiling of the gills to air exposure in mud crab Scylla paramamosain

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