Biochemical and morphological alterations caused by silver nanoparticles in Wistar rats

Sulaiman, Faoziyat Adenike; Adeyemi, Oluyomi Stephen; Akanji, Musbau Adewumi; Oloyede, Hussein Oyelola Bukoye; Sulaiman, Afolabi A.; Olatunde, Ahmed; Hoseni, Abiola A.; Olowolafe, Yemisi V.; Nlebedim, Regina N.; Muritala, H.F.; Nafiu, Mikhail Olugbemiro; Salawu, Musa Oyewole

doi:10.1016/j.jacme.2015.09.005

Cited by 41 publications

(23 citation statements)

References 21 publications

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“…Previous experiments have reported the potential of NPs to sequester in tissues and thus cause cellular injury. 2 Our findings indicated that administration of TiO 2 NPs led to histological changes in the liver, including irregular structure of hepatocyte cells, necrosis, apoptosis features, portal inflammation, degeneration, and congestion of the sinusoids. A significant reduction in hepatocytes count per unit area was detected in TiO 2 NP-treated rats.…”

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confidence: 51%

“…These properties lead to their extensive use in food and non-food industries, therapeutic processes, coating materials, consumer products, and fluorescent labels. [1][2][3] Titanium dioxide nanoparticles (TiO 2 NPs) are widely used in industries such as paper and also in plastics, paints, ceramics, cosmetics, water/air purifiers, agricultural chemicals, toothpastes, and sunscreens. 3,4 They are also used in the food industry as whiteners in sauces and creamers.…”

Section: Introductionmentioning

confidence: 99%

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<p>NLRP3 inflammasome, oxidative stress, and apoptosis induced in the intestine and liver of rats treated with titanium dioxide nanoparticles: in vivo and in vitro study</p>

Abbasi-Oshaghi¹,

Mirzaei²,

Pourjafar³

2019

IJN

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Purpose This study evaluated the effects of titanium dioxide nanoparticles (TiO 2 NPs) on liver and intestine of normal rats. Methods Male rats were divided into four groups as follows: 1) control rats, 2) control rats that orally received 10 mg/kg TiO 2 NPs, 3) control rats that orally received 50 mg/kg TiO 2 NPs, and 4) control rats that orally received 100 mg/kg TiO 2 NPs. After 30 days, the NLRP3 inflammasome pathway (NLRP3, caspase-1, and IL-1β), antioxidant pathway (superoxide dismutase [SOD], glutathione peroxidase [GPx], and catalase [CAT]), inflammatory pathway (inducible nitric oxide synthase [iNOS] and tumor necrosis factor-α [TNF-α]), and the apoptosis pathway (p53, Bax, Bcl-2, and caspase-3) were determined in the intestine and liver of the rats. H&E and Masson’s trichrome (MT) staining as well as TUNEL assay were used to examine the liver and the intestine. Biochemical factors, cytotoxicity, ROS generation, and apoptosis rate were also determined in HepG2 and Caco-2 cells. Results TiO 2 NPs in a dose-dependent manner increased cytotoxicity, oxidative stress, and apoptosis rate in Caco-2 and HepG2 cells. The administration of TiO 2 NPs significantly reduced antioxidant enzyme activity and gene expressions (SOD, CAT, and GPx) as well as glutathione (GSH) levels and total antioxidant capacity (TAC) in a dose-dependent manner. TiO 2 NPs also induced the apoptosis pathway and inflammatory pathway gene expressions and caspase-3 activity in the intestine and liver. TUNEL assay was in agreement with gene expressions. TiO 2 NPs also led to morphological changes in the liver and intestine. Conclusion TiO 2 NPs could have cytotoxic effects on the intestine and liver structure and function by inducing oxidative stress, inflammation, and apoptosis.

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mentioning

confidence: 51%

Section: Introductionmentioning

confidence: 99%

<p>NLRP3 inflammasome, oxidative stress, and apoptosis induced in the intestine and liver of rats treated with titanium dioxide nanoparticles: in vivo and in vitro study</p>

Abbasi-Oshaghi¹,

Mirzaei²,

Pourjafar³

2019

IJN

View full text Add to dashboard Cite

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“…As these changes did not occur in the present study, the proposed adverse changes resulting from altered blood parameters in previous studies might not have occurred. Determination of a lipid profile previously by Sulaiman et al () in an acute study conducted on Wistar rats after 24 hours of Ag‐NP administration led to an increase in atherogenic index showing depleted HDL levels with elevated levels of total cholesterol, triacylglycerides, free glycerol, low density lipoprotein‐cholesterol and bilirubin when compared with the control. The lipid profile determined in the present study revealed no significant changes with respect to control animals in both the acute and subacute studies, showing no threat and no possibility of a rise in the atherogenic index.…”

Section: Discussionmentioning

confidence: 91%

“…Determination of a lipid profile previously by Sulaiman et al (2015) in an acute study conducted on Wistar rats after 24 hours of Ag-NP administration led to an increase in atherogenic index showing depleted HDL levels with elevated levels of total cholesterol, triacylglycerides, free glycerol, low density lipoprotein-cholesterol and bilirubin when compared with the control. The lipid profile determined in the present study revealed no significant changes with FIGURE 7 Alterations in non-enzymatic antioxidant activity after 28 days of subacute oral treatment with CuO nanoparticles.…”

Section: Discussionmentioning

confidence: 99%

Acute and subacute oral toxicity of copper oxide nanoparticles in female albino Wistar rats

Bugata

Venkata

Gundu

et al. 2019

J of Applied Toxicology

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The extensive use of copper oxide nanoparticles (CuO‐NPs) in various industries and their wide range of applications have led to their accumulation in different ecological niches of the environment. This excess exposure raises the concern about its potential toxic effects on various organisms including humans. However, the hazardous potential of CuO‐NPs in the literature is elusive, and it is essential to study its toxicity in different biological models. Hence, we have conducted single acute dose (2000 mg/kg) and multiple dose subacute (30, 300 and 1000 mg/kg daily for 28 days) oral toxicity studies of CuO‐NPs in female albino Wistar rats following OECD guidelines 420 and 407 respectively. Blood analysis, tissue aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and acetylcholinesterase, superoxide dismutase, catalase, lipid malondialdehyde and reduced glutathione assays, and histopathology of the tissues were carried out. The higher dose treatments of the acute and subacute study caused significant alterations in biochemical and antioxidant parameters of the liver, kidney and brain tissues of the rat. In addition, histopathological evaluation of these three organs of treated rats showed significantly high abnormalities in their histoarchitecture when compared to control rats. We infer from the results that the toxicity observed in the liver, kidney and brain of treated rats could be due to the increased generation of reactive oxygen species by CuO‐NPs.

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“…However, Sulaiman et al (2015), reported that there was significant reduction in serum Cr and BUN levels after oral ingestion of silver NPs for 30 days in doses of (10, 50, and 100 mg/kg). The increase in Cr and BUN could be elucidated through possible inflammatory effects of sliver NPs as well as generation of reactive oxygen species as represented by elevated MDA level and reduction of SOD and GPx levels in the studied groups in relation to the control group (Yarmohammadi et al, 2014).…”

Section: A B C D a Bmentioning

confidence: 99%

Silver Nanotoxicity on Kidneys and Ovaries of Female Albino Rats

Nabeh

Taalab

Wahab

et al. 2020

Mansoura Journal of Forensic Medicine and Clinical Toxicology

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Silver nanoparticles (AgNPs) are widely involved in the medical products owing to their antibacterial activity. However, their extensive use causes human hazards via dermal, inhalational and parenteral routes. The aim of the current study was to investigate the toxic effect of different doses of AgNPs on the ovaries and the kidneys of the adult female Albino Rats. Forty female rats were randomly allocated to three treated groups and a control to assess the toxic effect of AgNPs injected intraperitoneally at 1, 2, 4 mg/kg respectively over a period of 28 days. Renal and ovarian tissues together with blood samples were collected and examined for biochemicals, hormonal and histopathological changes. The results revealed a decrease in Luteinizing hormone (LH) and Follicle-Stimulating hormone (FSH) in addition to increased levels of Blood Urea Nitrogen (BUN) and serum creatinine among the studied groups in comparison to the control. Furthermore, there were considerable generations of reactive oxygen species (ROS) expressed by elevated plasma malonaldehyde (MDA) level and decreased antioxidant glutathione peroxidase (GPx) and antioxidants-superoxide dismutase (SOD) levels together with demonstration of histopathological alterations in the renal and ovarian tissues in the three studied groups compared to the control group. Conclusively, renal and ovarian tissues displayed both functional and histopathological structural alteration following exposure to AgNPs in a characteristic dose-dependent manner. This can be partly explained by generation of ROS indicated by elevation of MDA level and reduction of GPx and SOD levels.

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Biochemical and morphological alterations caused by silver nanoparticles in Wistar rats

Cited by 41 publications

References 21 publications

<p>NLRP3 inflammasome, oxidative stress, and apoptosis induced in the intestine and liver of rats treated with titanium dioxide nanoparticles: in vivo and in vitro study</p>

<p>NLRP3 inflammasome, oxidative stress, and apoptosis induced in the intestine and liver of rats treated with titanium dioxide nanoparticles: in vivo and in vitro study</p>

Acute and subacute oral toxicity of copper oxide nanoparticles in female albino Wistar rats

Silver Nanotoxicity on Kidneys and Ovaries of Female Albino Rats

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