Biochemical and Molecular Studies on Bone Marrow Derived Stromal Stem Cells on Liver Injuries in Rats

Abstract

Liver is a remarkable vital organ, it is responsible for metabolizing the different food elements, filtering and detoxifying poisons in the blood to remove numerous toxic compounds. Liver affections are essentially caused by harmful chemicals (certain anti-infection, paracetamol, carbon tetrachloride, aflatoxins, per oxidized oil and so on.), overabundance utilization of liquor, immune system issue and disease. Up till now, orthotropic liver transplantation is the most powerful treatment of liver infections. Because of the deficiency of donors, high costs, the number of patients who can profit by this methodology is extremely constrained. Recently, cell based treatments have been explored as other option to entire liver transplantation. Thirty rats were separated into three equivalent groups (n=10, each). Group 1: control; Group 2: was infused peritoneally with acetaminophen (300 mg/kg); Group 3: was injected intra peritoneal with acetaminophen (300 mg/kg), then injected intravenous with mesenchymal stromal stem cells (1 million BM-MSCs/kg). Blood samples for estimation of liver functions, and liver samples for detection of homing labeled MSCs with red fluorescent cell linker dye (PKH26), gene expression of matrix metalopeptidase (MMP2), tissue inhibitor metalopeptidase (TIMP) and histopathological examination were collected. The infusion of BM-MSCs enhanced liver capacities test, MMP2 and TIMP. Histopathological examination of liver tissue demonstrated critical antifibrotic impact, improvement in the hepatic structure as compared to the second group. It could be concluded that BM-MSC is effective in treatment of liver damage by upgrading hepatocyte recovery through enhancing the liver stress and inflammatory signaling by improving MMP2 and TIMP, besides it has a significant antifibrotic effect. The MSCs stem cell is an attractive cell source for regenerative medicine.

Stem Cell-Based Therapies for Liver Diseases: An Overview and Update

“…Dowidar and colleagues [43] demonstrated that the transplantation of bone marrow-derived MSCs (BM-MSCs) could treat acetaminophen liver injury by the enhancement of hepatocyte regeneration and inhibition of liver stress and inflammatory signaling in the rat model. Besides BM-MSCs, researchers have found that adipose tissue-derived MSCs (AT-MSCs) also have hepatic differentiation potential in vitro and in vivo [44,45].…”

Regenerative Potential of Mesenchymal Stem Cells’ (MSCs) Secretome for Liver Fibrosis Therapies

“…ASCs are multipotent stem cells and have a more limited cell differentiation potential than ESCs. ASCs considered for liver regeneration are liver stem cells (LSCs) [13,14] and mesenchymal stem cells (MSCs) [15,16] from different tissue sources, such as bone marrow (BM-MSCs) [17], adipose tissue (ADSCs) [18][19][20], umbilical cords (UC-SCs), peripheral blood, cartilage, etc. MSCs show low immunogenicity, have a self-renewal ability, are easily obtainable, and can be used without ethical issues, which make them the perfect candidate for liver regeneration [21].…”